Enhancement and Molecular Identification of Wheat Germplasm Resources with Resistance Gene Pm30 to Powdery Mildew

doi:10.7668/hbnxb.2017.S1.002

Abstract

Abstract: Wheat powdery mildew is one of the main diseases threaten wheat production in China even all over the world. Screening and development disease-resistant varieties is a safe and effective way for prevention and control of wheat powdery mildew. The purpose of this study is to introduce powdery mildew resistance gene Pm30 into the wheat varieties Jizi 356 with advanced comprehensive agronomic characters and high yield developed by authors. One crosses,Jizi 356/Synthetics He-48 were made. Twelve lines with field resistance to powdery mildew and excellent agronomic characters were developed after six years of offspring screening. Then,seven lines were used for identifying resistance gene Pm30 using Xgwm159 SSR marker. Chinese spring and Pm30 were used as a contrast. The results showed that two new lines 14YF650 and 14YF675 could amplify the 430,460,500 bp polymorphic fragments. It indicated the two lines carrying Pm30 powdery mildew resistance gene derived from wild emmer. Those lines with big spikes,resistant to lodging and excellent agronomic characters could be used directly as parents for wheat powdery mildew resistance breeding.

Key words: Common wheat, Wild emmer, Resistance gene, Powdery mildew, Pm30 gene, Molecular marker

CLC Number:

Q78
S512.03

LAN Suque, LI Guangwei, MENG Yaning, LI Fang, ZHANG Yelun, LI Xingpu. Enhancement and Molecular Identification of Wheat Germplasm Resources with Resistance Gene Pm30 to Powdery Mildew[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(S1): 7-11. doi: 10.7668/hbnxb.2017.S1.002.

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References

[1] Bennett F A. Resistance to powdery mildew in wheat:A review of its use in agriculture and plant breeding programs[J].Plant Pathology,1984,33(3):279-300.
[2] Griffey C A,Das M K,Stromberg E L. Effectiveness of adult-plant resistance in reducing grain yield loss to powdery mildew in winter wheat[J]. Plant Disease,1993,77(6):618-622.
[3] 刘万才,邵振润.我国小麦白粉病大区流行的气候因素分析[J].植保技术与推广,1998,18(1):3-5.
[4] 宋伟.小麦品种良星99和汶农14的抗白粉病基因分子定位[D].秦皇岛:河北科技师范学院,2014.
[5] 全国农业技术推广中心病害预报处.2011年全国小麦重大病虫害发生趋势预报[J]. 种业导刊, 2011(2):13-14.
[6] 全国农业技术推广服务中心.2012年全国小麦重大病虫害发生趋势预报[J]. 种业导刊, 2012(2):15-16.
[7] 全国农业技术推广服务中心.2013年全国小麦重大病虫害发生趋势预报[J]. 种业导刊, 2013(3):7-8.
[8] 全国农业技术推广服务中心.2014年全国小麦重大病虫害发生趋势预报[J]. 种业导刊, 2014(2):14-15.
[9] 全国农业技术推广服务中心.2015年全国小麦重大病虫害发生趋势预报[J]. 种业导刊, 2015(2):13-14.
[10] 陈秀梅,曹远银,宋晶晶,等.我国部分麦区2011-2012年小麦白粉病菌小种及毒力分析[J].麦类作物学报,2013,33(3):584-588.
[11] 何方.小麦-长穗偃麦草杂种后代的分子细胞遗传学分析及种质材料的筛选鉴定[D].泰安:山东农业大学,2014.
[12] Mohler V,Bauer C,Schweizer G,et al. Pm50:A new powdery mildew resistance gene in common wheat derived from cultivated emmer[J]. Journal of Applied Genetics,2013,54(3):259-263.
[13] Petersen S,Lyerly J H,Worthington M L,et al.Mapping of powdery mildew resistance gene Pm53 introgressed from Aegilops speltoides into soft red winter wheat[J].Theoretical and Applied Genetics,2015,128(2):303-312.
[14] Hao Y F,Parks R,Cowger C,et al. Molecular characterization of a new powdery mildew resistance gene Pm54 in soft red winter wheat[J]. Theoretical and Applied Genetics,2015,128(3):465-476.
[15] 庄巧生.中国小麦品种改良及系谱分析[M].北京:中国农业出版社,2003:473-474.
[16] Liu Zhiyong,Sun Qixin,Ni Zhongfu,et al. Molecular characterization of a novel powdery mildew resistance gene Pm30 in wheat originating from wild emmer[J]. Euphytica 2002,123(1):21-29.
[17] 盛宝钦,段霞瑜.小麦白粉病成株抗性鉴定"0-9级"法修改意见[J].北京农业科学,1991(1):38-39.
[18] Ma Z Q,Wei J B,Cheng S H. PCR-based markers for the powdery mildew resistance gene Pm4a in wheat[J]. Theor Appl Genet,2004,109(1):140-145.
[19] Evans S, Lagudah,Simon G,Krattinger,et al. Gene-speci markers for the wheat gene Lr34/Yr18/Pm38 which confers resistance to multiple fungal pathogens[J]. Theor Appl Genet,2009,119(5):889-898.
[20] 阳霞,刘路平,孙道杰,等.小麦抗病基因Lr46/Yr29/Pm39,Sr2/Yr30和Lr68 的遗传特性及其与主要农艺性状的关联分析[J].西北植物学报,2014,34(3):454-462.
[21] He R,Chang Z,Yang Z,et al. Inheritance and mapping of powdery mildew resistance gene Pm43 introgressed from Thinopyrum intermedium into wheat[J]. Theoretical and Applied Genetics. 2009,118(6):1173-1180.
[22] Xiao M,Song F,Jiao J,et al. Identification of the gene Pm47 on chromosome 7BS conferring resistance to powdery mildew in the Chinese wheat landrace Hongyanglazi[J]. Theoretical and Applied Genetics,2013,126(5):1397-1403.
[23] 曹世勤,骆惠生,武翠平,等.甘肃省主要小麦生产品种(系)及抗源材料抗白粉病基因推导分析[J].作物学报,2010,36(12):2107-2115.
[24] 江峥,王琪琳,吴建辉,等.基于基因特异性标记分析Pm21在中国冬小麦品种(系)中的分布[J].中国农业科学,2014,47(11):2078-2087.
[25] 张海泉.小麦抗白粉病分子育种研究进展[J].中国生态农业学报,2008,16(4):1060-1066.
[26] Wis'niewska H,Kowalczyk K.Resistance of cultivars and breeding lines of spring wheat to Fusarium culmorum and powdery mildew[J]. Journal of Applied Genetics,2005,46(1):35-40.
[27] 高安礼,何华纲,陈全战,等.分子标记辅助选择小麦抗白粉病基因Pm2,Pm4a,Pm21 的聚合体[J].作物学报,2005,31(11):1400-1405.
[28] 张增艳,陈孝,张超,等.分子标记选择小麦抗白粉病基因Pm4b、Pm13和Pm21聚合体[J].中国农业科学,2002,35(7):789-793.
[29] 董建力,王敬东,惠红霞,等.小麦3个抗白粉病基因聚合于推广品种后代抗性的遗传分析[J].华北农学报,2007,22(2):161-163.

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