Identification of Leaf Rust Resistance Genes in 40 CIMMYT Wheat Lines at Seedling Stage and Adult Plant

doi:10.7668/hbnxb.20192637

Abstract

Abstract:

To identify leaf rust resistance genes in 40 wheat materials from the International Maize and Wheat Improvement Center (CIMMYT), combined with pedigree analysis, gene derivation and molecular marker detection, 36 known disease resistance gene vector varieties and 40 wheat materials were vaccinated with 17 physiological species of leaf rust bacteria with different toxicity.By comparing the infected types of the tested wheat lines and the known rust resistance genes,the possible known resistance genes in the tested wheat materials could be postulated.At the same time,12 markers closely linked with known resistance genes were used to detect the 40 CIMMYT lines,and the results could be verified with the gene postulation.Furthermore,40 tested lines were planted in Baoding,Hebei Province and Zhoukou,Henan Province,respectively,and inoculated with mixed Pt races for leaf rust evaluation at adult plant stage in the field.The results showed that 7 wheat lines contained Lr1,Lr10 was found in 9 lines,and each of Lr11 and Lr34 was present in 10 lines.Each of Lr14a,Lr15 and Lr26 was postulated to present in 2,4 and 3 lines,respectively.Each of Lr37 and Lr46 was identified in 22,39 wheat lines,respectively,by using molecular marker detection.In the field test 22 wheat lines showed adult plant resistance to leaf rust.

Key words: Wheat leaf rust, Leaf rust resistance gene, Gene postulation, Adult plant leaf rust resistance, Molecular marker detection

WANG Jiarong, DONG Rui, ZHANG Mengyu, GAO Pu, ZHANG Peipei, LI Zaifeng, LIU Daqun. Identification of Leaf Rust Resistance Genes in 40 CIMMYT Wheat Lines at Seedling Stage and Adult Plant[J]. Acta Agriculturae Boreali-Sinica, 2022, 37(2): 201-210. doi: 10.7668/hbnxb.20192637.

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URL: http://www.hbnxb.net/EN/10.7668/hbnxb.20192637

http://www.hbnxb.net/EN/Y2022/V37/I2/201

Figures/Tables 7

Tab.1 The pedigrees and origins of the 40 wheat lines

序号 No.	品系 Lines	系谱 Pedigree	来源 Origin
1	XYZ 461	WAXWING/7/TNMU/6/CEP80111/CEP81165/5/IAC5/4/YKT406/3/AG/ASN//ATR/8/ATTILA/3*BCN//BAV92/3/TILHI/4/SHA7/VEE#5//ARIV92	CIMMYT
2	XYZ 462	ATTILA2/HUITES//FINSI/3/ATTILA 2/PBW65/4/TRCH/SRTU//KACHU	CIMMYT
3	XYZ 463	WBLL1* 2/4/YACO/PBW65/3/KAUZ* 2/TRAP//KAUZ/5/KACHU #1/6/TRCH/SRTU//KACHU	CIMMYT
4	XYZ 464	TIMBA/KACHU/4/MARCHOUCH* 4/SAADA/3/2* FRET2/KUKUNA//FRET2	CIMMYT
5	XYZ 465	SAUAL/3/KAUZ/PASTOR//PBW343/4/TRCH/SRTU//KACHU	CIMMYT
6	XYZ 466	TRCH/SRTU//KACHU/3/TRCH/SRTU//KACHU	CIMMYT
7	XYZ 467	TILHI/SOKOLL* 2//KINGBIRD #1	CIMMYT
8	XYZ 468	TRCH/SRTU//KACHU* 2/3/PVN	CIMMYT
9	XYZ 469	TRCH/SRTU//KACHU* 2/3/PVN	CIMMYT
10	XYZ 470	TRCH/SRTU//KACHU* 2/3/PVN	CIMMYT
11	XYZ 471	TRCH/SRTU//KACHU* 2/3/KINGBIRD #1	CIMMYT
12	XYZ 472	TRCH/SRTU//KACHU* 2/3/KINGBIRD #1	CIMMYT
13	XYZ 473	WBLL1/KUKUNA//TACUPETO F2001/3/BAJ #1* 2/4/KINGBIRD #1	CIMMYT
14	XYZ 474	KIRITATI/2* WBLL1/5/FRET2/KUKUNA//FRET2/3/YANAC/4/FRET2/KIRITATI/6/BECARD #1	CIMMYT
15	XYZ 475	ROLF07/YANAC//TACUPETO F2001/BRAMBLING/4/WBLL1/KUKUNA//TACUPETO F2001/3/BAJ #1/5/WBLL1/KUKUNA//TACUPETO F2001/3/BAJ #1	CIMMYT
16	XYZ 476	FRET2/KUKUNA//FRET2/3/YANAC/4/FRET2/KIRITATI* 2/5/WHEAR//2* PRL/2* PASTOR	CIMMYT
17	XYZ 477	TRCH/5/BAV92//IRENA/KAUZ/3/HUITES/4/DOLL/6/TRCH/SRTU//KACHU	CIMMYT
18	XYZ 478	WHEAR/KUKUNA/3/C80.1/3* BATAVIA//2* WBLL1* 2/6/WBLL1* 2/4/YACO/PBW65/3/KAUZ* 2/TRAP//KAUZ/5/KACHU #1	CIMMYT
19	XYZ 479	PBW343* 2/KUKUNA//PBW343* 2/KUKUNA* 2/5/PFAU/SERI.1B//AMAD/3/INQALAB 91* 2/KURUKU/4/INQALAB 91* 2/KUKUNA	CIMMYT
20	XYZ 480	PBW343* 2/KUKUNA//PBW343* 2/KUKUNA* 2/6/WBLL1* 2/4/SNI/TRAP#1/3/KAUZ* 2/TRAP//KAUZ/5/KACHU	CIMMYT
21	XYZ 481	PBW343* 2/KUKUNA//PBW343* 2/KUKUNA* 2/6/C80.1/3* BATAVIA//2* WBLL1/5/REH/HARE//2* BCN/3/CROC_1/AE.SQUARROSA (213)//PGO/4/HUITES	CIMMYT
22	XYZ 482	MELON//FILIN/MILAN/3/FILIN/4/TRCH/SRTU//KACHU/5/TRCH/SRTU//KACHU	CIMMYT
23	XYZ 483	PAURAQ/2* NELOKI	CIMMYT
24	XYZ 484	BECARD/KINGBIRD #1//BECARD	CIMMYT
25	XYZ 485	SOKOLL/3/PASTOR//HXL7573/2* BAU/4/2* PASTOR//HXL7573/2* BAU/3/SOKOLL/WBLL1	CIMMYT
26	XYZ 486	SOKOLL/3/PASTOR//HXL7573/2* BAU* 2/4/PAURAQ	CIMMYT
27	XYZ 487	SOKOLL/3/PASTOR//HXL7573/2* BAU* 2/4/EGA BONNIE ROCK	CIMMYT
28	XYZ 488	SOKOLL/3/PASTOR//HXL7573/2* BAU* 2/4/EGA BONNIE ROCK	CIMMYT
29	XYZ 489	SOKOLL/3/PASTOR//HXL7573/2* BAU* 2/4/EGA BONNIE ROCK	CIMMYT
30	XYZ 490	SOKOLL/3/PASTOR//HXL7573/2* BAU* 2/4/GLADIUS	CIMMYT
31	XYZ 491	SOKOLL/3/PASTOR//HXL7573/2* BAU* 2/4/GLADIUS	CIMMYT
32	XYZ 492	WBLL1* 2/BRAMBLING//ND643/2* WBLL1	CIMMYT
33	XYZ 493	ND643/2* WBLL1/4/HUW234+LR34/PRINIA//PBW343* 2/KUKUNA/3/ROLF07	CIMMYT
34	XYZ 494	MERCATO/4/FRAME//MILAN/KAUZ/3/PASTOR/5/WHEAR/SOKOLL	CIMMYT
35	XYZ 495	BABAX/LR42//BABAX/3/ER2000/11/CROC_1/AE.SQUARROSA(213)//PGO/10/ATTILA* 2/9/KT/BAGE//FN/U/3/BZA/4/TRM/5/ALDAN/6/SERI/7/VEE#10/8/OPATA/12/BAVIS	CIMMYT
36	XYZ 496	ATTILA* 2/PBW65//BERKUT/3/WHEAR/SOKOLL	CIMMYT
37	XYZ 497	ND643/2* WBLL1* 2//KINGBIRD #1	CIMMYT
38	XYZ 498	PFAU/MILAN/3/BABAX/LR42//BABAX* 2/4/NIINI #1	CIMMYT
39	XYZ 499	PFAU/MILAN/3/BABAX/LR42//BABAX* 2/4/NIINI #1	CIMMYT
40	XYZ 500	PBW343* 2/KUKUNA//JUCHI/3/ATTILA* 2/PBW65//TNMU	CIMMYT

Tab.1 The pedigrees and origins of the 40 wheat lines

序号 No.	品系 Lines	系谱 Pedigree	来源 Origin
1	XYZ 461	WAXWING/7/TNMU/6/CEP80111/CEP81165/5/IAC5/4/YKT406/3/AG/ASN//ATR/8/ATTILA/3*BCN//BAV92/3/TILHI/4/SHA7/VEE#5//ARIV92	CIMMYT
2	XYZ 462	ATTILA2/HUITES//FINSI/3/ATTILA 2/PBW65/4/TRCH/SRTU//KACHU	CIMMYT
3	XYZ 463	WBLL1* 2/4/YACO/PBW65/3/KAUZ* 2/TRAP//KAUZ/5/KACHU #1/6/TRCH/SRTU//KACHU	CIMMYT
4	XYZ 464	TIMBA/KACHU/4/MARCHOUCH* 4/SAADA/3/2* FRET2/KUKUNA//FRET2	CIMMYT
5	XYZ 465	SAUAL/3/KAUZ/PASTOR//PBW343/4/TRCH/SRTU//KACHU	CIMMYT
6	XYZ 466	TRCH/SRTU//KACHU/3/TRCH/SRTU//KACHU	CIMMYT
7	XYZ 467	TILHI/SOKOLL* 2//KINGBIRD #1	CIMMYT
8	XYZ 468	TRCH/SRTU//KACHU* 2/3/PVN	CIMMYT
9	XYZ 469	TRCH/SRTU//KACHU* 2/3/PVN	CIMMYT
10	XYZ 470	TRCH/SRTU//KACHU* 2/3/PVN	CIMMYT
11	XYZ 471	TRCH/SRTU//KACHU* 2/3/KINGBIRD #1	CIMMYT
12	XYZ 472	TRCH/SRTU//KACHU* 2/3/KINGBIRD #1	CIMMYT
13	XYZ 473	WBLL1/KUKUNA//TACUPETO F2001/3/BAJ #1* 2/4/KINGBIRD #1	CIMMYT
14	XYZ 474	KIRITATI/2* WBLL1/5/FRET2/KUKUNA//FRET2/3/YANAC/4/FRET2/KIRITATI/6/BECARD #1	CIMMYT
15	XYZ 475	ROLF07/YANAC//TACUPETO F2001/BRAMBLING/4/WBLL1/KUKUNA//TACUPETO F2001/3/BAJ #1/5/WBLL1/KUKUNA//TACUPETO F2001/3/BAJ #1	CIMMYT
16	XYZ 476	FRET2/KUKUNA//FRET2/3/YANAC/4/FRET2/KIRITATI* 2/5/WHEAR//2* PRL/2* PASTOR	CIMMYT
17	XYZ 477	TRCH/5/BAV92//IRENA/KAUZ/3/HUITES/4/DOLL/6/TRCH/SRTU//KACHU	CIMMYT
18	XYZ 478	WHEAR/KUKUNA/3/C80.1/3* BATAVIA//2* WBLL1* 2/6/WBLL1* 2/4/YACO/PBW65/3/KAUZ* 2/TRAP//KAUZ/5/KACHU #1	CIMMYT
19	XYZ 479	PBW343* 2/KUKUNA//PBW343* 2/KUKUNA* 2/5/PFAU/SERI.1B//AMAD/3/INQALAB 91* 2/KURUKU/4/INQALAB 91* 2/KUKUNA	CIMMYT
20	XYZ 480	PBW343* 2/KUKUNA//PBW343* 2/KUKUNA* 2/6/WBLL1* 2/4/SNI/TRAP#1/3/KAUZ* 2/TRAP//KAUZ/5/KACHU	CIMMYT
21	XYZ 481	PBW343* 2/KUKUNA//PBW343* 2/KUKUNA* 2/6/C80.1/3* BATAVIA//2* WBLL1/5/REH/HARE//2* BCN/3/CROC_1/AE.SQUARROSA (213)//PGO/4/HUITES	CIMMYT
22	XYZ 482	MELON//FILIN/MILAN/3/FILIN/4/TRCH/SRTU//KACHU/5/TRCH/SRTU//KACHU	CIMMYT
23	XYZ 483	PAURAQ/2* NELOKI	CIMMYT
24	XYZ 484	BECARD/KINGBIRD #1//BECARD	CIMMYT
25	XYZ 485	SOKOLL/3/PASTOR//HXL7573/2* BAU/4/2* PASTOR//HXL7573/2* BAU/3/SOKOLL/WBLL1	CIMMYT
26	XYZ 486	SOKOLL/3/PASTOR//HXL7573/2* BAU* 2/4/PAURAQ	CIMMYT
27	XYZ 487	SOKOLL/3/PASTOR//HXL7573/2* BAU* 2/4/EGA BONNIE ROCK	CIMMYT
28	XYZ 488	SOKOLL/3/PASTOR//HXL7573/2* BAU* 2/4/EGA BONNIE ROCK	CIMMYT
29	XYZ 489	SOKOLL/3/PASTOR//HXL7573/2* BAU* 2/4/EGA BONNIE ROCK	CIMMYT
30	XYZ 490	SOKOLL/3/PASTOR//HXL7573/2* BAU* 2/4/GLADIUS	CIMMYT
31	XYZ 491	SOKOLL/3/PASTOR//HXL7573/2* BAU* 2/4/GLADIUS	CIMMYT
32	XYZ 492	WBLL1* 2/BRAMBLING//ND643/2* WBLL1	CIMMYT
33	XYZ 493	ND643/2* WBLL1/4/HUW234+LR34/PRINIA//PBW343* 2/KUKUNA/3/ROLF07	CIMMYT
34	XYZ 494	MERCATO/4/FRAME//MILAN/KAUZ/3/PASTOR/5/WHEAR/SOKOLL	CIMMYT
35	XYZ 495	BABAX/LR42//BABAX/3/ER2000/11/CROC_1/AE.SQUARROSA(213)//PGO/10/ATTILA* 2/9/KT/BAGE//FN/U/3/BZA/4/TRM/5/ALDAN/6/SERI/7/VEE#10/8/OPATA/12/BAVIS	CIMMYT
36	XYZ 496	ATTILA* 2/PBW65//BERKUT/3/WHEAR/SOKOLL	CIMMYT
37	XYZ 497	ND643/2* WBLL1* 2//KINGBIRD #1	CIMMYT
38	XYZ 498	PFAU/MILAN/3/BABAX/LR42//BABAX* 2/4/NIINI #1	CIMMYT
39	XYZ 499	PFAU/MILAN/3/BABAX/LR42//BABAX* 2/4/NIINI #1	CIMMYT
40	XYZ 500	PBW343* 2/KUKUNA//JUCHI/3/ATTILA* 2/PBW65//TNMU	CIMMYT

Tab.2 Seedling infection types of 36 lines with known leaf rust resistance genes inoculated with 17 Puccinia triticina (Pt)races

序号 No.	Lr 基因 Lr genes	叶锈菌生理小种侵染型Infection types to Pt pathotypes
序号 No.	Lr 基因 Lr genes	FGJQ	FHJQ①	FHJT	FHJR	TGPS	FHJQ②	THDB	PHTS	FHJQ③	FHSQ	FHGS①	FHDS	FHGS②	FHDQ	FHJS	FGJQ	FGDQ
1	RL6003(Lr1)	;1	;1	;1	2	3+	;	3+	4	;1	;1	0	0	;	;1	1	1	;1
2	RL6016(Lr2a)	;1	;	2	1	3	1	3+	;1	;1	1	;	0	;	;1	2	1	;1
3	RL6047(Lr2C)	3+	3+	3+	3+	3	3	3	3+	3	3+	3	3	3	3	3	4	3
4	RL6002(Lr3)	3+	3+	3+	3+	3	3+	3	3+	3	3	3	3	3	3	3	3	3
5	RL6010(Lr9)	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
6	RL6005(Lr16)	3+	3+	3+	3+	3+	3+	3+	3	3+	3	3	4	3+	4	4	3+	4
7	RL6064(Lr24)	;1	;	;1	;	;	;	;1	;1	;1	;	;	;1	;	0	1	;	;1
8	RL6078(Lr26)	1	3+	3	3	2+	3+	3	3	3	3	3	3	3	3	3	1	1
9	RL6007(Lr3ka)	2	2	2	2	3+	1	2	3	1	3	;	1	2	1	1	2	1
10	RL6053(Lr11)	3+	3	3+	3+	2+	3	2	3+	3+	3+	3	2	3	2	3	3	2
11	RL6008(Lr17)	3	3	3+	3+	3+	3+	3+	3	3	3+	2	3	2+	3	3	3	3
12	RL6049(Lr30)	1+	1+	1	2	3	1	2	3+	1	2	1	2	2	2	2	1	2
13	RL6051(LrB)	3C	3+	3+	3+	3C	3	3	3+	3+	3	3	3	3+	4	4	4	3+
14	RL6004(Lr10)	3C	3	3+	4	3+	3+	1	3	3+	3	3	3	3+	4	3	3+	4
15	RL6013(Lr14a)	1	2	3	X	3+	2	1+	3	2	X	3	3+	3	2+	3	2	1+
16	RL6009(Lr18)	1	2	3	3	2	2	2	2	2	1	0	0	0	0	2	2	2+
17	RL6019(Lr2b)	2	2+	2	2	3+	2	3+	2	3	3	;1	3	3	2	2	2	3
18	RL6042(Lr3bg)	3	3	3+	3+	4	3+	3+	3+	3+	3	3	3	3+	3+	3	4	4
19	RL4031(Lr13)	3	3	3+	3	3+	3	3	3	3	3	3C	3	3+	4	4	4	3+
20	RL6006(Lr14b)	3+	3+	3+	4	3	3+	3+	4	4	3+	4	3+	3+	4	3	4	4
21	RL605(Lr15)	;	0	0	3+	3+	3	3+	3	0	0	0	1	1	0	0	0;	0
22	RL604(Lr19)	;	;	0	0	;	0	0	0	0	0	0	;1	0	;	0	0;	0
23	RL609(Lr20)	;	;	3+	3	3	1	3+	3+	;	3	;	1	3+	4	4	;	2
24	RL604(Lr21)	1+	2	2	3	2	2	1+	1	1	2	2	3	2	2	2	2+	2
25	RL601(Lr23)	3	3+	3+	3+	3+	3+	3+	3	3+	3	3	3+	3+	4	3	3+	4
26	RL607(Lr28)	0	;	0	0	0	0	0	0	0	0	;	;1	0	;	1	;	0
27	RL608(Lr29)	;	2	2	1	2	1	1+	1	1	1	2	;	1	0	2	1	2
28	RL605(Lr33)	3C	3	3+	3	3	3+	3+	3+	3+	3	3	3+	3+	3	3	3	3
29	E8401(Lr36)	1+	1+	2	1	1	1	1	3	2	1	1	1	2	2	2	2	2
30	KS86NGRC02(Lr39)	3	3+	3	3	3	3+	3+	3+	3+	3	3	3	3+	4	3	3+	3
31	KS91WGRC11(Lr42)	;	1+	1	2	1	1	1	12	1	2	1	1	2	;	1	;	1
32	RL614(Lr44)	3	3	3+	2	3	2	3	4	3+	3C	3	2	3	3	3	3	3
33	RL614(Lr45)	3	3+	3	3+	4	4	3C	2	3+	3	3+	3	3+	2	4	4	3+
34	C98.00(Lr47)	0	0	0	0	0	0	;	0	0	;	;	;1	0	0	0	0	;
35	C78.5(Lr51)	;	;	;1	;	;	;	;	;	;	;	;	;1	;	1	;1	0;	;
36	98M71(Lr53)	1	;	;	;1	1	1	;	;	;	;	;	;1	;	;	;	;	;

Tab.2 Seedling infection types of 36 lines with known leaf rust resistance genes inoculated with 17 Puccinia triticina (Pt)races

序号 No.	Lr 基因 Lr genes	叶锈菌生理小种侵染型Infection types to Pt pathotypes
序号 No.	Lr 基因 Lr genes	FGJQ	FHJQ①	FHJT	FHJR	TGPS	FHJQ②	THDB	PHTS	FHJQ③	FHSQ	FHGS①	FHDS	FHGS②	FHDQ	FHJS	FGJQ	FGDQ
1	RL6003(Lr1)	;1	;1	;1	2	3+	;	3+	4	;1	;1	0	0	;	;1	1	1	;1
2	RL6016(Lr2a)	;1	;	2	1	3	1	3+	;1	;1	1	;	0	;	;1	2	1	;1
3	RL6047(Lr2C)	3+	3+	3+	3+	3	3	3	3+	3	3+	3	3	3	3	3	4	3
4	RL6002(Lr3)	3+	3+	3+	3+	3	3+	3	3+	3	3	3	3	3	3	3	3	3
5	RL6010(Lr9)	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
6	RL6005(Lr16)	3+	3+	3+	3+	3+	3+	3+	3	3+	3	3	4	3+	4	4	3+	4
7	RL6064(Lr24)	;1	;	;1	;	;	;	;1	;1	;1	;	;	;1	;	0	1	;	;1
8	RL6078(Lr26)	1	3+	3	3	2+	3+	3	3	3	3	3	3	3	3	3	1	1
9	RL6007(Lr3ka)	2	2	2	2	3+	1	2	3	1	3	;	1	2	1	1	2	1
10	RL6053(Lr11)	3+	3	3+	3+	2+	3	2	3+	3+	3+	3	2	3	2	3	3	2
11	RL6008(Lr17)	3	3	3+	3+	3+	3+	3+	3	3	3+	2	3	2+	3	3	3	3
12	RL6049(Lr30)	1+	1+	1	2	3	1	2	3+	1	2	1	2	2	2	2	1	2
13	RL6051(LrB)	3C	3+	3+	3+	3C	3	3	3+	3+	3	3	3	3+	4	4	4	3+
14	RL6004(Lr10)	3C	3	3+	4	3+	3+	1	3	3+	3	3	3	3+	4	3	3+	4
15	RL6013(Lr14a)	1	2	3	X	3+	2	1+	3	2	X	3	3+	3	2+	3	2	1+
16	RL6009(Lr18)	1	2	3	3	2	2	2	2	2	1	0	0	0	0	2	2	2+
17	RL6019(Lr2b)	2	2+	2	2	3+	2	3+	2	3	3	;1	3	3	2	2	2	3
18	RL6042(Lr3bg)	3	3	3+	3+	4	3+	3+	3+	3+	3	3	3	3+	3+	3	4	4
19	RL4031(Lr13)	3	3	3+	3	3+	3	3	3	3	3	3C	3	3+	4	4	4	3+
20	RL6006(Lr14b)	3+	3+	3+	4	3	3+	3+	4	4	3+	4	3+	3+	4	3	4	4
21	RL605(Lr15)	;	0	0	3+	3+	3	3+	3	0	0	0	1	1	0	0	0;	0
22	RL604(Lr19)	;	;	0	0	;	0	0	0	0	0	0	;1	0	;	0	0;	0
23	RL609(Lr20)	;	;	3+	3	3	1	3+	3+	;	3	;	1	3+	4	4	;	2
24	RL604(Lr21)	1+	2	2	3	2	2	1+	1	1	2	2	3	2	2	2	2+	2
25	RL601(Lr23)	3	3+	3+	3+	3+	3+	3+	3	3+	3	3	3+	3+	4	3	3+	4
26	RL607(Lr28)	0	;	0	0	0	0	0	0	0	0	;	;1	0	;	1	;	0
27	RL608(Lr29)	;	2	2	1	2	1	1+	1	1	1	2	;	1	0	2	1	2
28	RL605(Lr33)	3C	3	3+	3	3	3+	3+	3+	3+	3	3	3+	3+	3	3	3	3
29	E8401(Lr36)	1+	1+	2	1	1	1	1	3	2	1	1	1	2	2	2	2	2
30	KS86NGRC02(Lr39)	3	3+	3	3	3	3+	3+	3+	3+	3	3	3	3+	4	3	3+	3
31	KS91WGRC11(Lr42)	;	1+	1	2	1	1	1	12	1	2	1	1	2	;	1	;	1
32	RL614(Lr44)	3	3	3+	2	3	2	3	4	3+	3C	3	2	3	3	3	3	3
33	RL614(Lr45)	3	3+	3	3+	4	4	3C	2	3+	3	3+	3	3+	2	4	4	3+
34	C98.00(Lr47)	0	0	0	0	0	0	;	0	0	;	;	;1	0	0	0	0	;
35	C78.5(Lr51)	;	;	;1	;	;	;	;	;	;	;	;	;1	;	1	;1	0;	;
36	98M71(Lr53)	1	;	;	;1	1	1	;	;	;	;	;	;1	;	;	;	;	;

Tab.3 Seedling infection types of the 40 wheat lines with 17 Puccinia triticina (Pt) races

序号 No.	品系 Lines	品种所含基因 Genes	叶锈菌生理小种侵染型Infection types to Pt pathotypes
序号 No.	品系 Lines	品种所含基因 Genes	FGJQ	FHJQ①	FHJT	FHJR	TGPS	FHJQ②	THDB	PHTS	FHJQ③	FHSQ	FHGS①	FHDS	FHGS②	FHDQ	FHJS	FGJQ	FGDQ
1	XYZ 461	$Lr 11 d 、 Lr 26 c 、 Lr 46 e$	;	1	;1	2	1	3	12	3	;1	;1	3	1	1	1	1	1	1
2	XYZ 462	$Lr 11 d 、 Lr 37 e 、 Lr 46 e$	2+	12	3	12	1	3	12	2	12	2+	2	1	1	1	1	1	1
3	XYZ 463	$Lr 37 e 、 Lr 46 e$	3	12	3	2+	3	3	12	3	2	2+	2	1	1	1	2	1	1
4	XYZ 464	$Lr 10 e 、 Lr 14 ac 、 Lr 34 e 、 Lr 37 e 、 Lr 46 e$	1	12	3	2	3	;1	1	3	1	2	1	1	1	1	1	1	1
5	XYZ 465	$Lr 26 c 、 Lr 37 e 、 Lr 46 e$	;	12	3	2+	2	3	1	3C	2	2+	2	1	1	1	1	;1	1
6	XYZ 466	$Lr 37 e 、 Lr 46 e$	3	2	3C	2+	3	2	1	3C	2	3C	2+	1	12	1	2	1	2
7	XYZ 467	$Lr 10 e 、 Lr 34 e 、 Lr 46 e$	3	;1	;1	;	1	;1	;	3	1	1	12	1	;	;	1	;	1
8	XYZ 468	$Lr 37 e 、 Lr 46 e$	3	1	3	3	3	3	3	3	3C	3	3	3	1	2	3	1	3
9	XYZ 469	$Lr 11 d 、 Lr 37 e 、 Lr 46 e$	;	0	;1	3	2	1	1	2+	;1	;1	3+	1	;	1	1	1	1
10	XYZ 470	$Lr 37 e 、 Lr 46 e$	;	3	3	3	3	2	1+	3C	;	1	3	1	;	1	1	;	1
11	XYZ 471	$Lr 10 e 、 Lr 37 e 、 Lr 46 e$	3	3C	3	2	3	3	1	3C	2+	3	2	2	1	1	1	2	1
12	XYZ 472	$Lr 10 e 、 Lr 37 e 、 Lr 46 e$	3C	2	3	1	3	2	;1	2+	2+	2	1	1	1	1	1	1	1
13	XYZ 473	$Lr 1 e 、 Lr 10 e 、 Lr 34 e 、 Lr 46 e$	;	;	;1	1	12	;1	1	2	;1	;1	1	1	;1	1	1	1	1
14	XYZ 474	$Lr 1 d 、 Lr 10 e 、 Lr 11 d 、 Lr 46 e$	1	2	1	2	1	2	12	3	2	2+	2	1	1	1	2	1	1
15	XYZ 475	$Lr 11 d 、 Lr 34 e 、 Lr 46 e$	3	1	1	1	;1	1	12	3-	;1	2	2+	2	1	2	1	1	1
16	XYZ 476	$Lr 34 e$	;	3	3+	2	;1	3	12	3C	3	;1	3	3	1	2	3	1	3
17	XYZ 477	$Lr 10 e 、 Lr 37 e 、 Lr 46 e$	2	1	2	2	;1	2	1+	2	;1	12	3	1	1	2	3	1	3
18	XYZ 478	$Lr 1 e 、 Lr 10 e 、 Lr 34 e 、 Lr 37 e 、 Lr 46 e$	;	;	;	1	;1	;1	1	;1	;1	;1	;	1	;1	1	1	1	1
19	XYZ 479	$Lr 1 e 、 Lr 34 e 、 Lr 46 e$	;1	;1	1	1	;	;1	;1	12	;1	;1	12	1	;1	1	1	1	1
20	XYZ 480	$Lr 1 e 、 Lr 34 e 、 Lr 37 e 、 Lr 46 e$	1	1	1	1	;	;1	12	2	12	;1	0	2	;	1	1	0	1
21	XYZ 481	$Lr 1 e 、 Lr 34 e 、 Lr 46 e$	1	1+	1	12	;	;1	12	;1	;1	;1	0	1	0	1	1	;	1
22	XYZ 482	$Lr 1 e 、 Lr 11 d 、 Lr 37 e 、 Lr 46 e$	1	1	2	2	;	1	1	2	1	1	1	2	1 2	1	1	;	1
23	XYZ 483	$Lr 11 d 、 Lr 46 e$	;	;1	1	1	;1	;1	1+	3	;1	1	1	1 2	1	1	1	1	1
24	XYZ 484	$Lr 11 d 、 Lr 37 e 、 Lr 46 e$	3	3	3	1	;1	;1	;	2	1	12	12	1	1	1	1	;	1
25	XYZ 485	$Lr 46 e$	3	3+	3+	2+	3+	3+	3	2+	3	3+	1	1	1	1	1	;	1
26	XYZ 486	$Lr 46 e$	3	3	3+	2	3+	3	1	2+	1+	12	1	1	1	1	1	1	1
27	XYZ 487	$Lr 46 e$	3+	3+	3	3	3	3	3+	3	3	1	1	2	3	0;	4	3+	1
28	XYZ 488	$Lr 15 d 、 Lr 46 e$	;	;	;	1	3	;1	3	;1	;1	1	1	1	;	1	0	;	1
29	XYZ 489	$Lr 15 d 、 Lr 46 e$	;	;	;	;1	3	3	3	;1	;1	1	1	1	;	1	0;	;	1
30	XYZ 490	$Lr 15 d 、 Lr 46 e$	;	;	;	2	4	;1	3+	;1	;	1	1	1	0	1	0	;	1
31	XYZ 491	$Lr 37 e 、 Lr 46 e$	4	3+	3+	3+	4	3+	3+	3+	4	1	3	3	4	4	4	4	1
32	XYZ 492	$Lr 46 e$	;1	3	3	1+	;1	1	3	;1	2	1	1	1	2	1	1	1	1
33	XYZ 493	$Lr 11 d 、 Lr 46 e$	1	1+	3	;	;1	3+	1	;1	;1	1	1	;	2	1	1	;	1
34	XYZ 494	$Lr 37 e 、 Lr 46 e$	3	2	3	3C	3+	3+	12	3	2+	2+	2+	2	3C	1	2	;	1
35	XYZ 495	$Lr 11 d 、 Lr 14 ac 、 Lr 26 c 、 Lr 37 e 、 Lr 46 e$	;1	;	;	;1	;1	;1	1	;1	1	12	1	;	1	1	3	;	1
36	XYZ 496	$Lr 37 e 、 Lr 46 e$	;	;	;	;1	;1	;1	;	;1	;	;	1	;	1	1	;	1	1
37	XYZ 497	$Lr 15 d 、 Lr 34 e 、 Lr 37 e 、 Lr 46 e$	1	;	;	3	3+	;1	1	3-	;1	1	1	;	1	1	;	1	1
38	XYZ 498	$Lr 37 e 、 Lr 46 e$	;	;	;	;	;	;	;1	;	;1	;	1	;	1	1	1	;	1
39	XYZ 499	$Lr 37 e 、 Lr 46 e$	;	;	;	1	;	;	;1	;	1	;	;1	;	1	1	1	;	1
40	XYZ 500	$Lr 10 e 、 Lr 15 d 、 Lr 46 e$	;	;	;	;	;	;	;1	;1	;	;1	;1	;	1	1	1	;	1

Tab.3 Seedling infection types of the 40 wheat lines with 17 Puccinia triticina (Pt) races

序号 No.	品系 Lines	品种所含基因 Genes	叶锈菌生理小种侵染型Infection types to Pt pathotypes
序号 No.	品系 Lines	品种所含基因 Genes	FGJQ	FHJQ①	FHJT	FHJR	TGPS	FHJQ②	THDB	PHTS	FHJQ③	FHSQ	FHGS①	FHDS	FHGS②	FHDQ	FHJS	FGJQ	FGDQ
1	XYZ 461	$Lr 11 d 、 Lr 26 c 、 Lr 46 e$	;	1	;1	2	1	3	12	3	;1	;1	3	1	1	1	1	1	1
2	XYZ 462	$Lr 11 d 、 Lr 37 e 、 Lr 46 e$	2+	12	3	12	1	3	12	2	12	2+	2	1	1	1	1	1	1
3	XYZ 463	$Lr 37 e 、 Lr 46 e$	3	12	3	2+	3	3	12	3	2	2+	2	1	1	1	2	1	1
4	XYZ 464	$Lr 10 e 、 Lr 14 ac 、 Lr 34 e 、 Lr 37 e 、 Lr 46 e$	1	12	3	2	3	;1	1	3	1	2	1	1	1	1	1	1	1
5	XYZ 465	$Lr 26 c 、 Lr 37 e 、 Lr 46 e$	;	12	3	2+	2	3	1	3C	2	2+	2	1	1	1	1	;1	1
6	XYZ 466	$Lr 37 e 、 Lr 46 e$	3	2	3C	2+	3	2	1	3C	2	3C	2+	1	12	1	2	1	2
7	XYZ 467	$Lr 10 e 、 Lr 34 e 、 Lr 46 e$	3	;1	;1	;	1	;1	;	3	1	1	12	1	;	;	1	;	1
8	XYZ 468	$Lr 37 e 、 Lr 46 e$	3	1	3	3	3	3	3	3	3C	3	3	3	1	2	3	1	3
9	XYZ 469	$Lr 11 d 、 Lr 37 e 、 Lr 46 e$	;	0	;1	3	2	1	1	2+	;1	;1	3+	1	;	1	1	1	1
10	XYZ 470	$Lr 37 e 、 Lr 46 e$	;	3	3	3	3	2	1+	3C	;	1	3	1	;	1	1	;	1
11	XYZ 471	$Lr 10 e 、 Lr 37 e 、 Lr 46 e$	3	3C	3	2	3	3	1	3C	2+	3	2	2	1	1	1	2	1
12	XYZ 472	$Lr 10 e 、 Lr 37 e 、 Lr 46 e$	3C	2	3	1	3	2	;1	2+	2+	2	1	1	1	1	1	1	1
13	XYZ 473	$Lr 1 e 、 Lr 10 e 、 Lr 34 e 、 Lr 46 e$	;	;	;1	1	12	;1	1	2	;1	;1	1	1	;1	1	1	1	1
14	XYZ 474	$Lr 1 d 、 Lr 10 e 、 Lr 11 d 、 Lr 46 e$	1	2	1	2	1	2	12	3	2	2+	2	1	1	1	2	1	1
15	XYZ 475	$Lr 11 d 、 Lr 34 e 、 Lr 46 e$	3	1	1	1	;1	1	12	3-	;1	2	2+	2	1	2	1	1	1
16	XYZ 476	$Lr 34 e$	;	3	3+	2	;1	3	12	3C	3	;1	3	3	1	2	3	1	3
17	XYZ 477	$Lr 10 e 、 Lr 37 e 、 Lr 46 e$	2	1	2	2	;1	2	1+	2	;1	12	3	1	1	2	3	1	3
18	XYZ 478	$Lr 1 e 、 Lr 10 e 、 Lr 34 e 、 Lr 37 e 、 Lr 46 e$	;	;	;	1	;1	;1	1	;1	;1	;1	;	1	;1	1	1	1	1
19	XYZ 479	$Lr 1 e 、 Lr 34 e 、 Lr 46 e$	;1	;1	1	1	;	;1	;1	12	;1	;1	12	1	;1	1	1	1	1
20	XYZ 480	$Lr 1 e 、 Lr 34 e 、 Lr 37 e 、 Lr 46 e$	1	1	1	1	;	;1	12	2	12	;1	0	2	;	1	1	0	1
21	XYZ 481	$Lr 1 e 、 Lr 34 e 、 Lr 46 e$	1	1+	1	12	;	;1	12	;1	;1	;1	0	1	0	1	1	;	1
22	XYZ 482	$Lr 1 e 、 Lr 11 d 、 Lr 37 e 、 Lr 46 e$	1	1	2	2	;	1	1	2	1	1	1	2	1 2	1	1	;	1
23	XYZ 483	$Lr 11 d 、 Lr 46 e$	;	;1	1	1	;1	;1	1+	3	;1	1	1	1 2	1	1	1	1	1
24	XYZ 484	$Lr 11 d 、 Lr 37 e 、 Lr 46 e$	3	3	3	1	;1	;1	;	2	1	12	12	1	1	1	1	;	1
25	XYZ 485	$Lr 46 e$	3	3+	3+	2+	3+	3+	3	2+	3	3+	1	1	1	1	1	;	1
26	XYZ 486	$Lr 46 e$	3	3	3+	2	3+	3	1	2+	1+	12	1	1	1	1	1	1	1
27	XYZ 487	$Lr 46 e$	3+	3+	3	3	3	3	3+	3	3	1	1	2	3	0;	4	3+	1
28	XYZ 488	$Lr 15 d 、 Lr 46 e$	;	;	;	1	3	;1	3	;1	;1	1	1	1	;	1	0	;	1
29	XYZ 489	$Lr 15 d 、 Lr 46 e$	;	;	;	;1	3	3	3	;1	;1	1	1	1	;	1	0;	;	1
30	XYZ 490	$Lr 15 d 、 Lr 46 e$	;	;	;	2	4	;1	3+	;1	;	1	1	1	0	1	0	;	1
31	XYZ 491	$Lr 37 e 、 Lr 46 e$	4	3+	3+	3+	4	3+	3+	3+	4	1	3	3	4	4	4	4	1
32	XYZ 492	$Lr 46 e$	;1	3	3	1+	;1	1	3	;1	2	1	1	1	2	1	1	1	1
33	XYZ 493	$Lr 11 d 、 Lr 46 e$	1	1+	3	;	;1	3+	1	;1	;1	1	1	;	2	1	1	;	1
34	XYZ 494	$Lr 37 e 、 Lr 46 e$	3	2	3	3C	3+	3+	12	3	2+	2+	2+	2	3C	1	2	;	1
35	XYZ 495	$Lr 11 d 、 Lr 14 ac 、 Lr 26 c 、 Lr 37 e 、 Lr 46 e$	;1	;	;	;1	;1	;1	1	;1	1	12	1	;	1	1	3	;	1
36	XYZ 496	$Lr 37 e 、 Lr 46 e$	;	;	;	;1	;1	;1	;	;1	;	;	1	;	1	1	;	1	1
37	XYZ 497	$Lr 15 d 、 Lr 34 e 、 Lr 37 e 、 Lr 46 e$	1	;	;	3	3+	;1	1	3-	;1	1	1	;	1	1	;	1	1
38	XYZ 498	$Lr 37 e 、 Lr 46 e$	;	;	;	;	;	;	;1	;	;1	;	1	;	1	1	1	;	1
39	XYZ 499	$Lr 37 e 、 Lr 46 e$	;	;	;	1	;	;	;1	;	1	;	;1	;	1	1	1	;	1
40	XYZ 500	$Lr 10 e 、 Lr 15 d 、 Lr 46 e$	;	;	;	;	;	;	;1	;1	;	;1	;1	;	1	1	1	;	1

Fig.1 Detection of partial PCR results amplified by 13 molecular markers in 40 foreign wheat varieties (lines)
M.DL2000;Z.Zhengzhou 5389;1.XYZ461;2.XYZ462;3.XYZ463;4.XYZ464;5.XYZ465;6.XYZ466;7.XYZ467;8.XYZ468;9.XYZ469;10.XYZ470;11.XYZ471.The+,-in the upper right corner of the Lr19 and Lr26 indicate that the marks used for the detection are different.

Fig.2 Molecular marker detection for Lr46 of wheat
M.DNA Marker;1.TcLr46;2.Zhengzhou 5389;3.XYZ461;4.XYZ462;5.XYZ463;6.XYZ464;7.XYZ465;8.XYZ466;9.XYZ467;10.XYZ468;11.XYZ469;12.XYZ470;13.XYZ471;14.XYZ472;15.XYZ473;16.XYZ474;17.XYZ475;18.XYZ476;19.XYZ477;20.XYZ478;21.XYZ479;22. XYZ480;23.XYZ481;24.XYZ482.

Tab.4 Seedling infection types and final disease severity(FDS)in field with mixed Puccinia triticina(Pt)races of 22 wheat lines with slow-rusting resistance

序号 No.	品系 Lines	成株期抗病基因 APR genes	苗期混合生理小种侵染型 Seedling ITs to mixed Pt isolates	2018—2019年周口FDS/% 2018—2019 Zhoukou FDS	2019—2020年保定FDS/% 2019—2020 Baoding FDS
1	XYZ461	Lr46	3	5	10
3	XYZ463	Lr37、Lr46	3	5	10
4	XYZ464	Lr37、Lr46	3	1	5
5	XYZ465	Lr34、Lr37、Lr46	3	10	10
6	XYZ466	Lr37、Lr46	3	5	5
7	XYZ467	Lr46	3	5	10
8	XYZ468	Lr34、Lr37、Lr46	3	5	5
9	XYZ469	Lr37、Lr46	3+	1	5
10	XYZ470	Lr37、Lr46	3	10	10
11	XYZ471	Lr37、Lr46	3	5	10
12	XYZ472	Lr37、Lr46	3	5	5
16	XYZ476	Lr34、Lr46	3+	10	10
17	XYZ477	Lr34、Lr37、Lr46	3	1	1
22	XYZ482	Lr34、Lr37、Lr46	3	10	10
23	XYZ483	Lr46	3	1	10
24	XYZ484	Lr46	3	10	10
26	XYZ486	Lr46	3+	1	10
27	XYZ487	Lr46	4	5	5
29	XYZ489	Lr46	3	10	10
30	XYZ490	Lr46	4	10	5
35	XYZ495	Lr37、Lr46	3	1	1
37	XYZ497	Lr37、Lr46	3+	5	1
SAAR			3+	1	5
郑州5389	Zhengzhou 5389		3+	80	90
LSD				11.4

Tab.5 Analysis of variance for the final disease severity of the 40 wheat lines,slow rusting check SAAR and susceptible check Zhengzhou 5389 in 2018—2019 and 2019—2020

差异源 Source of variation	自由度 df	均方差 MS	F值 F value	P
品系 Lines	59	3 842.052 5	59.18^*	0.000 1
环境 Environment	1	1 118.016 7	17.22^*	0.000 1
重复 Repetition	1	220.416 7	3.39	0.770 3
品系×环境 Lines×Environment	59	113.465 8	1.17^*	0.006 5
品系×重复 Lines×Repetition	59	49.272 6	0.76	0.854 7
误差 Error	60	64.925 0

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