Establishment of MITE Amplification System in the Wheat Study

doi:10.7668/hbnxb.2014.02.021

Abstract

Abstract: Thatcher and 23 of wheat leaf rust resistance near isogenic lines in Thatcher background were chosen as materials in this experiment. Research on the establishment of MITE amplification system was carried out. 27 of the 56 primer pairs were amplified stably, reaction system and different annealing temperature of primers were con- firmed for amplification of MITE (Miniature inverted repeat transposable elements) .

Key words: Wheat leaf rust, Resistance gene, MITE, Molecular markers

CLC Number:

S512.03

XIE Huan, YANG Wen-xiang, PENG Qiao-hui, ZHAO Li-juan, LIU Li-wei, ZHANG Na, LIU Da-qun. Establishment of MITE Amplification System in the Wheat Study[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(2): 110-115. doi: 10.7668/hbnxb.2014.02.021.

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References

[1] Komer J A. Genetics of resistance to wheat leaf rust[J].Annual Review of Phytopathology, 1996, 34 (9): 435 -455.
[2] Singh D, Mohler V, Park R F. Discovery, characterisationand mapping of wheat leaf rust resistance gene Lr71[J].Euphytica. 2013,190(1):131-136.
[3] 胡亚亚, 张娜, 李林懋, 等. 14 个小麦品种(系) 抗叶锈性分析[J]. 作物学报,2011,37(12):2158-2166.
[4] Bureau T E, Wessler S R. Tourist: A large family of smallinverted repeat elements frequently associated with maizegenes[J]. The Plant Cell,1992,4(10):1283-1294.
[5] Bureau T E, Wessler S R. Stowaway: A new family of in-verted repeat elements associated with the genes of mono-cotyledonous and dicotyledonous plants [J ]. The PlantCell,1994,6(6):907-916.
[6] Jiang N, Feschotte C D, Zhang X, et al. Using rice to un-derstand the origin and amplification of miniature invertedrepeat transposable elements(MITEs) [J]. Current Opin-ion in Plant Biology,2004,7(4):115-119.
[7] Komori T, Nitta N. High frequency of sequence polymor-phism in rice MITEs an application to efficient develop-ment of PCR-based markers[J]. Breeding Science,2003,53(2):85-92.
[8] Chang R Y, O' Donoughue L S, Bureau T E. Inter-MITEpolymorphisms (IMP ): a high throughput transposon-based genome mapping and fingerprinting approach[J].Theoretical and Applied Genetics, 2001, 102(5 ): 773 -781.
[9] Lu C, Chen J, Zhang Y, et al. Miniature inverted-repeattransposable elements (MITEs) have been accumulatedthrough amplification bursts and play important roles ingene expression and species diversity in Oryza sativa[J].Molecular Biology and Evolution, 2012, 29 (3 ): 1005 -1017.
[10] Amundsen K, Rotter D, Li H M, et al. Miniature invert-ed-repeat transposable element identification and geneticmarker development in Agrostis[J]. Crop Science,2011,51(2),854-861.
[11] 马忠友, 苏京平, 孙林静, 等. 微型反向重复转座元件(MITE) 靶区域扩增多态性: 一种基于 MITE 的分子标记方法在水稻及其他植物上的应用[J]. 中国水稻科学,2007,21(5):459-463.
[12] 高玉龙, 桂毅杰, 肖炳光, 等. 烟草 MITE 位点间多态性(IMP) 标记开发及其遗传作图应用[J]. 浙江大学学报: 农业与生命科学版,2012,38(6):655-661.
[13] 贾继增. 分子标记种质资源鉴定和分子标记育种[J]. 中国农业科学,1996,29(4):1-10.
[14] Madan Mohan. Genome mapping, molecular marker andmarker assisted selection in crop plants [J]. MolecularBreeding,1997,3(2):87-103.
[15] Sakai H, Tanaka T, Itoh T. Birth and death of genes pro-moted by transposable elements in Oryza sativa [J].Gene,2007,392(1-2):59-63.
[16] Guillet-Claude C, Birolleau-Touchard C, Manicacci D, etal. Nucleotide diversity of the Zmpox3 maize peroxidasegene: relationships between a MITE insertion in exon 2and variation in forage maize digestibility [J]. BiomedCentral Genetics,2004,5(19):1-11.

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