Abstract: A high-frequency transformation system has been established by Agrobacterium tumefaciens transfor- mation technology and using meristems of axillary buds as transformation receptor. External factors that affect the ef- ficiency of genetic transformation on the base of gentamicin were studied and optimized, such as the bacterial con- centration and the times of transformation and co-cultivation. The major results were as follows: seedling was grown for 20 days, then axillary buds excised and stabbed were infected with Agrobacterium tumefaciens which OD600 was 0. 7 for 10 min. Then after co-cultivation for 2 days, transformed plants were selected by 20 mg/L and 40 mg/L gen- tamicin on the leaves and rooting respectively. It was originally proved by the PCR that expression cassettes had been transferred into the genome. The positive transformation rate was 76% by the analysis of PCR. And the living rate of the transplanted transgenic plants was 76. 3% .

Key words: Melon, Plant apical meristem, Genetic transformation, Agrobacterium tumefaciens

CLC Number: 

• S652