Analysis of Disease Resistance and Physiological Characteristics of Transgenic Tobacco with Solanum tuberosum StPR1 Gene

doi:10.7668/hbnxb.20190650

Abstract

Abstract: A plant expression vector pBI121 -StPR1 was constructed to clarify the role of potato StPR1 gene in disease resistance, and introduce StPR1 gene into tobacco by Agrobacterium transformation method. The disease resistance and physiological characteristics of transgenic tobacco were investigated using different pathogen treatment, including bacterial disease soft rot (E. carotovora subsp. Carotovora Borgey, Ecc; E. chrysanthemi Burkholder. Atroseptica Dye, Ech; E. carotovora subsp. Mc Fadden et Dimock, Eca), bacterial wilt (Ralstonia solanacearum, RS) and fungal disease dry rot (F. sambucinum, F. avenaceum). The results showed that the diameter of lesions leaves increased with the prolongation of stress time in transgenic tobacco and wild type (WT) under the pathogen stress, and the diameter of transgenic tobacco lesions were significantly smaller than WT. In the physiological characteristics analysis, the physiological indexes of leaves increased with the prolongation of stress time in WT and transgenic tobacco, but the activities of SOD, POD and CAT in transgenic tobacco were all increased to different degrees compared WT. The results of disease resistance and physiological characteristics showed that transgenic tobacco had stronger resistance to pathogenic bacteria and fungi, indicating that the StPR1 gene played an important role in the disease resistance of potato, which could provide a theoretical basis for PR1 gene resistance in plants.

Key words: Solanum tuberosum, StPR1, Tobacco, Genetic transformation, Disease resistance analysis

CLC Number:

Q78
S532

HE Fumeng, LI Xiuyu, ZHAO Xiaocan, WU Jiawen, ZHU Yuanfang, ZHOU Lei, SHI Qihai, LIU Di, LI Fenglan. Analysis of Disease Resistance and Physiological Characteristics of Transgenic Tobacco with Solanum tuberosum StPR1 Gene[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(3): 184-192. doi: 10.7668/hbnxb.20190650.

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References

[1] 陈彦云, 曹君迈, 陈晓军, 任晓月, 刘喜平, 丁红瑾. 我国马铃薯贮藏加工产业现状及其发展建议[J]. 保鲜与加工, 2011, 11(6):47-49. doi:10.3969/j.issn.1009-6221.2011.06.013.
Chen Y Y, Cao J M, Chen X J, Ren X Y, Liu X P, Ding H J. Present situation analysis and development suggestions on potato storage and processing industry of China[J]. Storage and Process, 2011, 11(6):47-49.
[2] Zhang H, Xu F, Wu Y, Hu H H, Dai X F. Progress of potato staple food research and industry development in China[J]. Journal of Integrative Agriculture, 2017, 16(12):2924-2932.
[3] 孙海军.东北地区马铃薯常见病害的发生及防治[J]. 吉林农业, 2016(15):92. doi:10.14025/j.cnki.jlny.2016.15.038.
Sun H J. Occurrence and control of common diseases of potato in Northeast China[J]. Jilin Agriculture, 2016(15):92.
[4] van Loon L C, Van Strien E A. The families of pathogenesis-related proteins, their activities, and comparative analysis of PR-1 type proteins[J]. Physiological and Molecular Plant Pathology, 1999, 55(2):85-97. doi:10.1006/pmpp.1999.0213.
[5] 刘纪元, 佟少明, 侯和胜. 植物病程相关蛋白研究进展[J]. 黑龙江信息科技, 2012(5):65. doi:10.3969/j.issn.1673-1328.2012.05.077.
Liu J Y, Tong S M, Hou H S. Advances in research on plant pathogenesis related proteins[J]. Heilongjiang Science and Technology Information, 2012(5):65.
[6] Nasser W, de Tapia M, Burkard G. Maize pathogenesis-related proteins:characterization and cellular distribution of 1,3-β-glucanases and chitinases induced by Brome mosaic virus infection or mercuric chloride treatment[J]. Physiological and Molecular Plant Pathology, 1990, 36(1):1-14. doi:10.1016/0885-5765(90)90087-E.
[7] Alexander D, Goodman R M, Gut-Rella M, Glascock C, Weymann K, Friedrich L, Maddox D, Ahl-Goy P, Luntz T, Ward E. Increased tolerance to two oomycete pathogens in transgenic tobacco expressing pathogenesis-related protein 1a[J]. Proceedings of the National Academy of Sciences of the United States of America, 1993, 90(15):7327-7331. doi:10.1073/pnas.90.15.7327.
[8] Chun S C, Chandrasekaran M. Chitosan and chitosan nanoparticles induced expression of pathogenesis-related proteins genes enhances biotic stress tolerance in tomato[J]. International Journal of Biological Macromolecules, 2019, 125:948-954. doi:10.1016/j.ijbiomac.2018.12.167.
[9] Srivastava N, Gonugunta V K, Puli M R, Raghavendra A S. Nitric oxide production occurs downstream of reactive oxygen species in guard cells during stomatal closure induced by chitosan in abaxial epidermis of Pisum sativum[J]. Planta, 2009, 229(4):757-765. doi:10.1007/s00425-008-0855-5.
[10] Wang L, Guo Z H, Zhang Y B, Wang Y J, Yang G, Yang L, Wang R Y, Xie Z K. Isolation and characterization of two distinct Class Ⅱ PR4 genes from the oriental lily hybrid Sorbonne[J]. Russian Journal of Plant Physiology, 2017, 64(5):707-717. doi:10.1134/S1021443717050132.
[11] 李秀钰, 贺付蒙, 韩瑛琪, 赵潇璨, 武佳文, 朱元芳, 周磊, 石奇海, 冯哲, 李凤兰. 马铃薯StPR1 基因克隆及表达特异性分析[J]. 华北农学报, 2019, 34(2):66-71. doi:10.7668/hbnxb.201751433.
Li X Y, He F M, Han Y Q, Zhao X C, Wu J W, Zhu Y F, Zhou L, Shi Q H, Feng Z, Li F L. Cloning and expression characteristics of Solanum tuberosum StPR1 gene[J]. Acta Agriculturae Boreali-Sinica, 2019, 34(2):66-71.
[12] 胡重怡, 郑少清, 陈兴江, 陈丽莉, 程艳.烟草无菌苗培养前的种子消毒技术研究[J]. 中国烟草科学, 2007, 28(2):45-46,51. doi:10.3969/j.issn.1007-5119.2007.02.012.
Hu Z Y, Zheng S Q, Chen X J, Chen L L, Cheng Y. Methods for seed sterilization before aseptic tobacco seedling culture[J]. Chinese Tobacco Science, 2007, 28(2):45-46,51.
[13] 律凤霞. 根癌农杆菌介导外源基因转化烟草体系的优化[J]. 安徽农业科学, 2010, 38(19):10065-10066. doi:10.13989/j.cnki.0517-6611.2010.19.097.
Lü F X. Optimization for agrobacterium tumefaciens-mediated transformation system for exogenous genes of tobacco[J]. Journal of Anhui Agricultural Sciences, 2010, 38(19):10065-10066.
[14] Kitajima S, Sato F. Plant pathogenesis-related proteins:molecular mechanisms of gene expression and protein function[J]. The Journal of Biochemistry, 1999, 125(1):1-8. doi:10.1093/oxfordjournals.jbchem.a022244.
[15] Cameron R K,Paiva N L,Lamb C J,Dixon R A.Accumulation of salicylic acid and PR-1 gene transcripts in relation to the systemic acquired resistance (SAR) response induced by Pseudomonas syringae pv. tomato in Arabidopsis[J]. Physiological and Molecular Plant Pathology, 1999, 55(2):121-130. doi:10.1006/pmpp.1999.0214.
[16] Zhang G, Li Y M, Zhang Y, Dong Y L, Wang X J, Wei G R, Huang L L, Kang Z S. Cloning and characterization of a pathogenesis-related protein gene TaPR10 from wheat induced by stripe rust pathogen[J]. Agricultural Sciences in China, 2010, 9(4):549-556. doi:10.1016/S1671-2927(09)60128-0.
[17] 张计育, 渠慎春, 薛华柏, 高志红, 郭忠仁, 章镇. 湖北海棠病程相关蛋白MhPR8 基因的克隆与表达[J]. 中国农业科学, 2012, 45(8):1568-1575. doi:10.3864/j.issn.0578-1752.2012.08.012.
Zhang J Y, Qu S C, Xue H B, Gao Z H, Guo Z R, Zhang Z. Isolation and expression of pathogenesis-related protein gene MhPR8 from Malus hupehensis[J]. Scientia Agricultura Sinica, 2012, 45(8):1568-1575.
[18] Sarowar S, Kim Y J,Kim E N, Kim K D, Hwang B K,Islam R,Shin J S. Overexpression of a pepper basic pathogenesis-related protein 1 gene in tobacco plants enhances resistance to heavy metal and pathogen stresses[J]. Plant Cell Reports, 2005, 24(4):216-224. doi:10.1007/s00299-005-0928-x.
[19] Shi W N, Hao L L, Li J, Liu D D, Gou X Q, Li H. The Gossypium hirsutum WRKY gene GhWRKY39-1 promotes pathogen infection defense responses and mediates salt stress tolerance in transgenic Nicotiana benthamiana[J]. Plant Cell Reports, 2014, 33(3):483-498. doi:10.1007/s00299-013-1548-5.
[20] 乔子辰. 棉花黄萎病菌和Harpin_Xoo蛋白对转hpal_Xoo基因棉部分抗病生理指标的影响[D]. 乌鲁木齐:新疆农业大学, 2009. doi:10.7666/d.y1506399.
Qiao Z C.Part physiological resistance indexs of transgenic hpal_Xoo cotton interacting with Verticillium dahliae and Harpin_Xoo[D]. Urumqi:Xinjiang Agricultural University, 2009.

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