Effects of Two Endophytic Fungi on Physiological and Biochemical Indexes and NAC Gene Expression in Tobacco under Drought Stress

doi:10.7668/hbnxb.20194526

Abstract

Abstract:

To investigate the effects of Piriformospora indica combined with Arbuscular mycorrhizal fungi (AMF) on drought resistance of tobacco,Yunyan 87 was inoculated with sterile water (CC),P.indica (CP),AMF (PC),P.indica and AMF (PP),and the contents of proline (Pro),superoxide dismutase (SOD),peroxidase (POD),malondialdehyde (MDA) and the expression of drought-related genes NTNAC1 and NAC4 in tobacco leaves were determined under stress in the form of natural drought.The results showed that P.indica and AMF could promote the growth of above-ground and underground parts of tobacco,and chlorosis of leaves and symptoms of their drought were slight.After 7 days of drought stress,the contents of Pro in tobacco leaves of CP,PC and PP groups were 1.39,1.59 and 1.78 times higher than those of CC in group,respectively.The activities of SOD and POD in tobacco leaves increased first and then decreased.The activities of SOD in CP,PC and PP groups were 1.15,1.22 and 1.33 times higher than those in CC group,POD activity was 1.33,1.46 and 1.85 times higher than those of CC group,respectively.The content of MDA in tobacco leaves was decreased by 21.98%,23.98% and 24.84% in CP,PC and PP groups respectively.The expression levels of NTNAC1 and NAC4 in tobacco leaves were up-regulated.The expression levels of NTNAC1 in CP,PC and PP groups were 3.37,3.88 and 5.07 times higher than those in CC group,the expression levels of NAC4 gene were 3.04,3.59 and 5.56 times higher than those of CC group,respectively.This study indicates that P.indica and AMF showed significant synergistic effects,which could significantly improve the drought resistance of tobacco.

Key words: Tobacco, Piriformospora indica, Arbuscular mycorrhizal fungi, Drought resistance

SUN Wenxiu, SHAO Chenyang, CHEN Yanyan, NIE Minghao, LI Zhen, CAO Yi, LIU Yingbao. Effects of Two Endophytic Fungi on Physiological and Biochemical Indexes and NAC Gene Expression in Tobacco under Drought Stress[J]. Acta Agriculturae Boreali-Sinica, 2024, 39(1): 113-119. doi: 10.7668/hbnxb.20194526.

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References 30

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基因登录号 Gene accession number	引物名称 Primers name	引物序列(5'—3') Sequence of primers (5'—3')
NM_001325686.1	Actin For	TGGTTAAGGCTGGATTTGCT
	Actin Rev	TGCATCCTTTGACCCATAC
XM_016622840.1	NtNAC1 For	GTTTATGGGCAGAAGGAGCAG
	NtNAC1 Rev	CCTGGGTGATTTGCCTTGTTT
XM_016656095.1	NAC4 For	GGCAGGCGTTGTAGTAGGTGA
	NAC4 Rev	GATGTGGCTTGTGAATGTCTCG

基因登录号 Gene accession number	引物名称 Primers name	引物序列(5'—3') Sequence of primers (5'—3')
NM_001325686.1	Actin For	TGGTTAAGGCTGGATTTGCT
	Actin Rev	TGCATCCTTTGACCCATAC
XM_016622840.1	NtNAC1 For	GTTTATGGGCAGAAGGAGCAG
	NtNAC1 Rev	CCTGGGTGATTTGCCTTGTTT
XM_016656095.1	NAC4 For	GGCAGGCGTTGTAGTAGGTGA
	NAC4 Rev	GATGTGGCTTGTGAATGTCTCG

处理 Treatment	主根长/cm Main root length	地下部分鲜质量/g Underground part fresh weight	地下部分干质量/g Underground part dry weight	地上部分鲜质量/g Aboveground part fresh weight	地上部分干质量/g Aboveground part dry weight
CC	5.18±0.52a	2.77±0.09a	0.13±0.29a	18.11±0.24b	1.24±0.16a
CP	6.73±0.09b	3.69±0.22a	0.26±0.39a	25.94±0.32b	2.51±0.27b
PC	6.51±0.17b	3.78±0.47b	0.27±0.51b	25.89±0.19c	2.53±0.44c
PP	7.69±0.03c	4.02±0.62b	0.31±0.19c	26.83±0.37c	2.80±0.26c

处理 Treatment	主根长/cm Main root length	地下部分鲜质量/g Underground part fresh weight	地下部分干质量/g Underground part dry weight	地上部分鲜质量/g Aboveground part fresh weight	地上部分干质量/g Aboveground part dry weight
CC	5.18±0.52a	2.77±0.09a	0.13±0.29a	18.11±0.24b	1.24±0.16a
CP	6.73±0.09b	3.69±0.22a	0.26±0.39a	25.94±0.32b	2.51±0.27b
PC	6.51±0.17b	3.78±0.47b	0.27±0.51b	25.89±0.19c	2.53±0.44c
PP	7.69±0.03c	4.02±0.62b	0.31±0.19c	26.83±0.37c	2.80±0.26c

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