Effects of Cultivation of Long-leaf Age Tobacco Seedlings on Dry Matter and Nutrient Accumulation and Fertilizer Use Efficiency of Flue-cured Tobacco in Field

doi:10.7668/hbnxb.20194090

Abstract

Abstract:

Define the cultivation measures of long-leaf tobacco seedlings in tobacco-rice rotation area to promote the early growth and rapid development of paddy-tobacco.Yunyan 87 was used as the material.The effects of three factors on dry matter and nutrient accumulation and distribution,and fertilizer use efficiency of flue-cured tobacco were studied,namely,the aperture of nursery site(136,200 pores),microbial agent(substrate with Miaoqiangzhuang microbial agent,substrate without microbial agent),and transplanting leaf age(8-leaf transplanting,10-leaf transplanting).The results showed that increasing the aperture of nursery site and adding microbial agent in the substrate to cultivate long-leaf tobacco seedlings could increase dry matter accumulation,improve dry matter distribution,increase nitrogen,phosphorus and potassium accumulation,promote fertilizer absorption efficiency,and increase fertilizer utilization rate and production efficiency;the contribution rates of aperture of nursery site,microbial agent,transplanting leaf age and the interaction of three factors to dry matter accumulation were 22.87%,27.73%,37.41% and 11.99%,respectively.The contribution rates to nitrogen accumulation were 20.34%,23.45%,33.62% and 22.59%,respectively.The contribution rates of phosphorus accumulation were 24.41%,27.81%,32.85% and 14.93%,respectively.The contribution rates of potassium accumulation were 15.48%,26.05%,34.61% and 23.86%,respectively.The contribution rates to nitrogen efficiency were 20.81%,44.67%,23.11% and 11.41%,respectively.The contribution rates of phosphorus fertilizer efficiency were 32.15%,31.66%,14.91% and 21.28%,respectively.The contribution rates of potassium fertilizer efficiency were 25.31%,38.71%,31.67% and 4.31%,respectively.In Hunan paddy-tobacco growing area,increasing the aperture of nursery site and adding microbial agents to the substrate can make up for the defects of two-stage seedling raising,and jointly improve nutrient uptake and fertilizer utilization efficiency,which has a certain popularization value in flue-cured tobacco production.

Key words: Flue-cured tobacco, Aperture of nursery site, Microbial agent, Transplanting leaf age, Accumulation and distribution of dry matter, Fertilizer utilization efficiency

ZHANG Yang, HUANG Qionghui, XIE Huiya, ZHANG Hongbing, CHEN Shunyao, CAI Qi, WANG Xinyue, QUAN Keying, XIA Bing, DENG Xiaohua. Effects of Cultivation of Long-leaf Age Tobacco Seedlings on Dry Matter and Nutrient Accumulation and Fertilizer Use Efficiency of Flue-cured Tobacco in Field[J]. Acta Agriculturae Boreali-Sinica, 2023, 38(S1): 280-291. doi: 10.7668/hbnxb.20194090.

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http://www.hbnxb.net/EN/Y2023/V38/IS1/280

Figures/Tables 7

Tab.1 Effects of the diameter of seedling tray,microbial agent and transplanting leaf age on accumulation and distribution of tobacco dry matter

取样时期 Sampling period		处理 Treatments		干物质总量 /(kg/hm²) Total dry matter			干物质量积累量/(kg/hm²) Dry matter accumulation														干物质分配比例/% Distribution of dry matter
取样时期 Sampling period		处理 Treatments		干物质总量 /(kg/hm²) Total dry matter			根Root					茎Stem					叶Leaf				根Root					茎Stem						叶Leaf
团棵期		A1		319.63±		9.60a			26.38±		1.90a		37.00±		1.50a			256.25±		6.93a		7.93±			1.07a			11.99±		2.34a			80.08±		2.21a
Resettling		A2		247.52±		8.41b			22.67±		1.38b		29.49±		1.03b			195.36±		6.38b		8.85±			1.98a			11.95±		1.30a			79.21±		1.59a
stage		B1		311.11±		9.54a			25.08±		1.22a		36.44±		2.19a			249.58±		7.87a		7.81±			1.67a			12.06±		2.07a			80.13±		1.31a
		B2		256.04±		7.08b			23.97±		1.43a		30.05±		1.53b			202.02±		7.51b		8.97±			2.27a			11.88±		1.69a			79.16±		2.37a
		C1		215.41±		5.27b			14.31±		2.45b		28.63±		1.36b			172.47±		5.73b		6.76±			1.10b			13.11±		1.41a			80.13±		1.52a
		C2		351.74±		7.13a			34.73±		3.61a		37.87±		1.67a			279.14±		5.77a		10.01±			1.20a			10.83±		1.49a			79.16±		2.24a
		A1B1C1		270.93±		4.50c			15.51±		3.03c		38.36±		1.48ab			217.06±		1.98bc		5.70±			0.60b			14.13±		0.74a			80.18±		1.35a
		A1B1C2		420.42±		3.57a			37.54±		3.85a		39.02±		1.52ab			343.86±		1.20a		8.92±			0.42ab			9.29±		0.16b			81.80±		0.25a
		A1B2C1		231.50±		1.17d			15.59±		2.45c		31.02±		1.43b			184.88±		1.05c		6.74±			1.09b			13.41±		1.99ab			79.86±		3.08a
		A1B2C2		355.66±		1.98b			36.88±		1.78a		39.60±		1.93ab			279.18±		1.73b		10.37±			1.29a			11.13±		2.17ab			78.51±		3.46a
	A2B1C1		196.68±		2.10de			14.27±		1.52c				25.41±		1.77c			157.00±	1.85 cd			7.36±	1.63ab			12.81±		1.94ab		79.84±			0.30a
	A2B1C2		356.40±		2.93b			33.00±		1.40b				42.98±		1.42a			280.42±	2.12b			9.28±	0.36ab			12.03±		0.83ab		78.70±			0.47a
	A2B2C1		162.53±		1.17e			11.88±		1.57c				19.72±		1.62c			130.93±	1.98d			7.27±	0.90ab			12.08±		1.10ab		80.66±			2.00a
	A2B2C2		274.48±		2.45c			31.52±		0.23b				29.87±		1.97b			213.10±	1.65bc			11.49±	0.19a			10.89±		1.54ab		77.63±			1.73a
	$η A 2$		0.883					0.463						0.494					0.900				0.323				0.000				0.091
	$η B 2$		0.814					0.072						0.415					0.845				0.432				0.008				0.109
	$η C 2$		0.964					0.963						0.597					0.965				0.857				0.545				0.110
	$η A × B × C 2$		0.044					0.011						0.203					0.011				0.005				0.114				0.084
打顶期	A1		3 373.82±		53.52a			654.20±		17.25a				1 000.56±		19.92a			1 719.05±	22.57a			19.31±	1.53a			29.43±		3.22a		51.27±			4.56a
Topping	A2		3 251.16±		42.09b			653.07±		97.50a				904.70±		28.16a			1 693.40±	19.01a			20.07±	2.11a			27.80±		2.22a		52.12±			2.52a
stage	B1		3 390.67±		34.25a			666.58±		88.99a				930.62±		19.47a			1 793.47±	44.72a			19.69±	2.32a			27.29±		2.25a		53.02±			2.99a
	B2		3 234.31±		31.31b			640.70±		12.72a				974.63±		18.36a			1 618.98±	13.08b			19.69±	1.32a			29.94±		2.78a		50.38±			3.83a
	C1		3 009.11±		41.90b			575.60±		69.42b				806.83±		33.47b			1 626.67±	19.40b			19.12±	1.96a			26.86±		1.63b		54.02±			1.46a
	C2		3 615.87±		41.71a			731.67±		47.65a				1 098.43±		19.25a			1 785.77±	57.14a			20.27±	1.59a			30.37±		2.67a		49.37±			3.61b
	A1B1C1		3 158.10±		5.70b			545.08±		5.48d				841.09±		21.58b			1 771.94±	19.60ab			17.26±	0.37a			26.63±		0.38b		56.11±			0.01a
	A1B1C2		3 799.54±		2.12a			753.31±		37.69a				1 165.40±		9.80a			1 880.84±	6.77a			19.83±	0.88a			30.68±		0.43ab		49.50±			0.45b
	A1B2C1		2 963.65±		8.35bc			565.54±		8.75d				789.53±		3.50c			1 608.59±	3.10b			19.08±	0.11a			26.65±		0.37b		54.28±			0.26ab
	A1B2C2		3 573.98±		12.48ab			752.90±		17.97a				1 206.23±		17.62a			1 614.86±	12.13b			21.07±	0.43a			33.75±		0.61a		45.18±			0.18b
	A2B1C1		3 093.34±		3.08b			681.62±		18.43c				773.52±		20.53c			1 638.20±	9.98b			22.04±	0.82a			25.01±		0.40b		52.96±			0.42ab
	A2B1C2		3 511.70±		9.74ab			686.32±		88.55c				942.48±		33.60b			1 882.90±	4.70a			19.65±	3.64a			26.86±		0.57b		53.50±			4.21ab
	A2B2C1		2 821.34±		7.90c			510.18±		13.53b				823.19±		35.24b			1 487.97±	9.13c			18.08±	0.04a			29.17±		0.55ab		52.75±			0.59ab
	A2B2C2		3 578.27±		7.55ab			734.17±		18.55ab				1 079.6±		21.23ab			1 764.51±	10.34ab			20.53±	0.91a			30.18±		1.16ab		49.30±			2.07b
	$η A 2$		0.533					0.000						0.899					0.032				0.129				0.779				0.114
	$η B 2$		0.649					0.202						0.652					0.607				0.000				0.904				0.550
	$η C 2$		0.965					0.902						0.988					0.562				0.250				0.943				0.792
	$η A × B × C 2$		0.393					0.577						0.001					0.054				0.317				0.561				0.024
圆顶期	A1		5 669.98±		73.72a			1 096.84±		21.97a				1 573.17±		65.82a			2 999.97±	65.77a			19.38±	1.30a			27.71±		0.81a		52.91±			1.64a
Round	A2		5 423.12±		63.82b			1 008.36±		48.20b				1 510.84±		88.93a			2 903.92±	12.21b			18.54±	2.00a			27.88±		1.41a		53.59±			1.22a
top stage	B1		5 844.65±		32.25a			1 116.58±		36.14a				1 636.70±		12.84a			3 091.38±	71.24a			19.09±	1.95a			28.01±		1.45a		52.91±			1.34a
	B2		5 248.44±		47.86b			988.62±		83.70b				1 447.32±		50.28b			2 812.51±	12.20b			18.84±	1.50a			27.58±		0.66a		53.59±			1.55a
	C1		5 411.24±		32.46b			990.80±		97.85b				1 521.69±		17.93a			2 898.74±	91.99b			18.33±	1.74a			28.11±		1.05a		53.57±			1.44a
	C2		5 681.86±		47.78a			1 114.39±		12.82a				1 562.32±		15.91a			3 005.15±	27.41a			19.59±	1.47a			27.48±		1.15a		52.93±			1.47a
	A1B1C1		5 812.37±		6.09ab			1 059.55±		8.62b				1 617.50±		6.47b			3 135.33±	9.00a			18.23±	0.52b			27.83±		0.76a		53.95±			1.27a
	A1B1C2		6 307.62±		3.38a			1 231.56±		6.24a				1 800.56±		4.65a			3 275.50±	7.96a			19.56±	1.73a			28.55±		0.41a		51.89±			2.15a
	A1B2C1		5 171.18±		7.52c			1 081.25±		5.40b				1 415.45±		3.68c			2 674.49±	9.24c			20.92±	0.53a			27.37±		0.16a		51.72±			0.37a
	A1B2C2		5 388.74±		4.90b			1 015.00±		2.75b				1 459.18±		6.42c			2 914.56±	9.07ab			18.84±	0.44b			27.08±		1.18a		54.09±			1.62a
	A2B1C1		5 544.83±		3.54ab			945.29±		7.10c				1 637.05±		2.18b			2 962.49±	11.62ab			17.04±	0.83b			29.54±		1.19a		53.42±			0.35a
	A2B1C2		5 713.79±		3.33ab			1 229.91±		4.54a				1 491.68±		9.48c			2 992.19±	7.69ab			21.53±	0.64a			26.11±		0.23a		52.37±			0.88a
	A2B2C1		5 116.57±		2.03c			877.14±		5.57c				1 416.77±		6.32c			2 822.66±	13.14b			17.14±	0.52b			27.70±		0.06a		55.18±			0.45a
	A2B2C2		5 317.29±		4.07b			981.09±		2.70c				1 497.87±		7.73c			2 838.33±	8.50b			18.45±	0.55b			28.17±		0.55a		53.38±			1.10a
	$η A 2$		0.636					0.636						0.524					0.254				0.347				0.029				0.139
	$η B 2$		0.911					0.785						0.910					0.742				0.044				0.157				0.141
	$η C 2$		0.677					0.773						0.319					0.295				0.542				0.289				0.125
	$η A × B × C 2$		0.146					0.044						0.703					0.029				0.002				0.607				0.373

Tab.1 Effects of the diameter of seedling tray,microbial agent and transplanting leaf age on accumulation and distribution of tobacco dry matter

取样时期 Sampling period		处理 Treatments		干物质总量 /(kg/hm²) Total dry matter			干物质量积累量/(kg/hm²) Dry matter accumulation														干物质分配比例/% Distribution of dry matter
取样时期 Sampling period		处理 Treatments		干物质总量 /(kg/hm²) Total dry matter			根Root					茎Stem					叶Leaf				根Root					茎Stem						叶Leaf
团棵期		A1		319.63±		9.60a			26.38±		1.90a		37.00±		1.50a			256.25±		6.93a		7.93±			1.07a			11.99±		2.34a			80.08±		2.21a
Resettling		A2		247.52±		8.41b			22.67±		1.38b		29.49±		1.03b			195.36±		6.38b		8.85±			1.98a			11.95±		1.30a			79.21±		1.59a
stage		B1		311.11±		9.54a			25.08±		1.22a		36.44±		2.19a			249.58±		7.87a		7.81±			1.67a			12.06±		2.07a			80.13±		1.31a
		B2		256.04±		7.08b			23.97±		1.43a		30.05±		1.53b			202.02±		7.51b		8.97±			2.27a			11.88±		1.69a			79.16±		2.37a
		C1		215.41±		5.27b			14.31±		2.45b		28.63±		1.36b			172.47±		5.73b		6.76±			1.10b			13.11±		1.41a			80.13±		1.52a
		C2		351.74±		7.13a			34.73±		3.61a		37.87±		1.67a			279.14±		5.77a		10.01±			1.20a			10.83±		1.49a			79.16±		2.24a
		A1B1C1		270.93±		4.50c			15.51±		3.03c		38.36±		1.48ab			217.06±		1.98bc		5.70±			0.60b			14.13±		0.74a			80.18±		1.35a
		A1B1C2		420.42±		3.57a			37.54±		3.85a		39.02±		1.52ab			343.86±		1.20a		8.92±			0.42ab			9.29±		0.16b			81.80±		0.25a
		A1B2C1		231.50±		1.17d			15.59±		2.45c		31.02±		1.43b			184.88±		1.05c		6.74±			1.09b			13.41±		1.99ab			79.86±		3.08a
		A1B2C2		355.66±		1.98b			36.88±		1.78a		39.60±		1.93ab			279.18±		1.73b		10.37±			1.29a			11.13±		2.17ab			78.51±		3.46a
	A2B1C1		196.68±		2.10de			14.27±		1.52c				25.41±		1.77c			157.00±	1.85 cd			7.36±	1.63ab			12.81±		1.94ab		79.84±			0.30a
	A2B1C2		356.40±		2.93b			33.00±		1.40b				42.98±		1.42a			280.42±	2.12b			9.28±	0.36ab			12.03±		0.83ab		78.70±			0.47a
	A2B2C1		162.53±		1.17e			11.88±		1.57c				19.72±		1.62c			130.93±	1.98d			7.27±	0.90ab			12.08±		1.10ab		80.66±			2.00a
	A2B2C2		274.48±		2.45c			31.52±		0.23b				29.87±		1.97b			213.10±	1.65bc			11.49±	0.19a			10.89±		1.54ab		77.63±			1.73a
	$η A 2$		0.883					0.463						0.494					0.900				0.323				0.000				0.091
	$η B 2$		0.814					0.072						0.415					0.845				0.432				0.008				0.109
	$η C 2$		0.964					0.963						0.597					0.965				0.857				0.545				0.110
	$η A × B × C 2$		0.044					0.011						0.203					0.011				0.005				0.114				0.084
打顶期	A1		3 373.82±		53.52a			654.20±		17.25a				1 000.56±		19.92a			1 719.05±	22.57a			19.31±	1.53a			29.43±		3.22a		51.27±			4.56a
Topping	A2		3 251.16±		42.09b			653.07±		97.50a				904.70±		28.16a			1 693.40±	19.01a			20.07±	2.11a			27.80±		2.22a		52.12±			2.52a
stage	B1		3 390.67±		34.25a			666.58±		88.99a				930.62±		19.47a			1 793.47±	44.72a			19.69±	2.32a			27.29±		2.25a		53.02±			2.99a
	B2		3 234.31±		31.31b			640.70±		12.72a				974.63±		18.36a			1 618.98±	13.08b			19.69±	1.32a			29.94±		2.78a		50.38±			3.83a
	C1		3 009.11±		41.90b			575.60±		69.42b				806.83±		33.47b			1 626.67±	19.40b			19.12±	1.96a			26.86±		1.63b		54.02±			1.46a
	C2		3 615.87±		41.71a			731.67±		47.65a				1 098.43±		19.25a			1 785.77±	57.14a			20.27±	1.59a			30.37±		2.67a		49.37±			3.61b
	A1B1C1		3 158.10±		5.70b			545.08±		5.48d				841.09±		21.58b			1 771.94±	19.60ab			17.26±	0.37a			26.63±		0.38b		56.11±			0.01a
	A1B1C2		3 799.54±		2.12a			753.31±		37.69a				1 165.40±		9.80a			1 880.84±	6.77a			19.83±	0.88a			30.68±		0.43ab		49.50±			0.45b
	A1B2C1		2 963.65±		8.35bc			565.54±		8.75d				789.53±		3.50c			1 608.59±	3.10b			19.08±	0.11a			26.65±		0.37b		54.28±			0.26ab
	A1B2C2		3 573.98±		12.48ab			752.90±		17.97a				1 206.23±		17.62a			1 614.86±	12.13b			21.07±	0.43a			33.75±		0.61a		45.18±			0.18b
	A2B1C1		3 093.34±		3.08b			681.62±		18.43c				773.52±		20.53c			1 638.20±	9.98b			22.04±	0.82a			25.01±		0.40b		52.96±			0.42ab
	A2B1C2		3 511.70±		9.74ab			686.32±		88.55c				942.48±		33.60b			1 882.90±	4.70a			19.65±	3.64a			26.86±		0.57b		53.50±			4.21ab
	A2B2C1		2 821.34±		7.90c			510.18±		13.53b				823.19±		35.24b			1 487.97±	9.13c			18.08±	0.04a			29.17±		0.55ab		52.75±			0.59ab
	A2B2C2		3 578.27±		7.55ab			734.17±		18.55ab				1 079.6±		21.23ab			1 764.51±	10.34ab			20.53±	0.91a			30.18±		1.16ab		49.30±			2.07b
	$η A 2$		0.533					0.000						0.899					0.032				0.129				0.779				0.114
	$η B 2$		0.649					0.202						0.652					0.607				0.000				0.904				0.550
	$η C 2$		0.965					0.902						0.988					0.562				0.250				0.943				0.792
	$η A × B × C 2$		0.393					0.577						0.001					0.054				0.317				0.561				0.024
圆顶期	A1		5 669.98±		73.72a			1 096.84±		21.97a				1 573.17±		65.82a			2 999.97±	65.77a			19.38±	1.30a			27.71±		0.81a		52.91±			1.64a
Round	A2		5 423.12±		63.82b			1 008.36±		48.20b				1 510.84±		88.93a			2 903.92±	12.21b			18.54±	2.00a			27.88±		1.41a		53.59±			1.22a
top stage	B1		5 844.65±		32.25a			1 116.58±		36.14a				1 636.70±		12.84a			3 091.38±	71.24a			19.09±	1.95a			28.01±		1.45a		52.91±			1.34a
	B2		5 248.44±		47.86b			988.62±		83.70b				1 447.32±		50.28b			2 812.51±	12.20b			18.84±	1.50a			27.58±		0.66a		53.59±			1.55a
	C1		5 411.24±		32.46b			990.80±		97.85b				1 521.69±		17.93a			2 898.74±	91.99b			18.33±	1.74a			28.11±		1.05a		53.57±			1.44a
	C2		5 681.86±		47.78a			1 114.39±		12.82a				1 562.32±		15.91a			3 005.15±	27.41a			19.59±	1.47a			27.48±		1.15a		52.93±			1.47a
	A1B1C1		5 812.37±		6.09ab			1 059.55±		8.62b				1 617.50±		6.47b			3 135.33±	9.00a			18.23±	0.52b			27.83±		0.76a		53.95±			1.27a
	A1B1C2		6 307.62±		3.38a			1 231.56±		6.24a				1 800.56±		4.65a			3 275.50±	7.96a			19.56±	1.73a			28.55±		0.41a		51.89±			2.15a
	A1B2C1		5 171.18±		7.52c			1 081.25±		5.40b				1 415.45±		3.68c			2 674.49±	9.24c			20.92±	0.53a			27.37±		0.16a		51.72±			0.37a
	A1B2C2		5 388.74±		4.90b			1 015.00±		2.75b				1 459.18±		6.42c			2 914.56±	9.07ab			18.84±	0.44b			27.08±		1.18a		54.09±			1.62a
	A2B1C1		5 544.83±		3.54ab			945.29±		7.10c				1 637.05±		2.18b			2 962.49±	11.62ab			17.04±	0.83b			29.54±		1.19a		53.42±			0.35a
	A2B1C2		5 713.79±		3.33ab			1 229.91±		4.54a				1 491.68±		9.48c			2 992.19±	7.69ab			21.53±	0.64a			26.11±		0.23a		52.37±			0.88a
	A2B2C1		5 116.57±		2.03c			877.14±		5.57c				1 416.77±		6.32c			2 822.66±	13.14b			17.14±	0.52b			27.70±		0.06a		55.18±			0.45a
	A2B2C2		5 317.29±		4.07b			981.09±		2.70c				1 497.87±		7.73c			2 838.33±	8.50b			18.45±	0.55b			28.17±		0.55a		53.38±			1.10a
	$η A 2$		0.636					0.636						0.524					0.254				0.347				0.029				0.139
	$η B 2$		0.911					0.785						0.910					0.742				0.044				0.157				0.141
	$η C 2$		0.677					0.773						0.319					0.295				0.542				0.289				0.125
	$η A × B × C 2$		0.146					0.044						0.703					0.029				0.002				0.607				0.373

Tab.2 Effects of the diameter of seedling tray,microbial agent and transplanting leaf age on N accumulation and distribution of flue-cured tobacco

取样时期 Sampling period		处理 Treatments		总氮量/(kg/hm²) Total N			氮积累量/(kg/hm²) N accumulation															氮分配比例/% N distribution
取样时期 Sampling period		处理 Treatments		总氮量/(kg/hm²) Total N			根Root					茎Stem					叶Leaf					根Root			茎Stem			叶Leaf
团棵期		A1		15.23±		3.03a			0.64±		0.31a			1.43±		0.20a			13.16±		2.80a		4.10±	1.65a		9.66±	1.88a		86.24±	2.72a
Resettling		A2		12.54±		1.61b			0.62±		0.27a			1.21±		0.25a			10.71±		1.38b		4.92±	1.94a		9.71±	1.78a		85.38±	2.40a
stage		B1		14.30±		3.43a			0.58±		0.20a			1.33±		0.27a			12.39±		3.14a		4.01±	0.82a		9.59±	2.21a		86.40±	2.26a
		B2		13.47±		1.94a			0.68±		0.35a			1.31±		0.23a			11.48±		1.69a		5.01±	2.37a		9.78±	1.33a		85.21±	2.76a
		C1		12.26±		1.69b			0.40±		0.06b			1.22±		0.21a			10.64±		1.63b		3.26±	0.39a		10.12±	2.10a		86.62±	2.04a
		C2		15.51±		2.65a			0.86±		0.20a			1.42±		0.24a			13.23±		2.59a		5.76±	1.77a		9.25±	1.35a		84.99±	2.81a
		A1B1C1		11.79±		2.58c			0.38±		0.09b			1.40±		0.20ab			10.01±		2.30c		3.24±	0.03b		11.97±	0.92a		84.80±	0.90a
		A1B1C2		19.03±		0.59a			0.74±		0.17ab			1.47±		0.04ab			16.82±		0.38a		3.88±	0.77b		7.72±	0.04c		88.41±	0.73a
		A1B2C1		14.08±		0.93b			0.43±		0.05ab			1.28±		0.01b			12.38±		0.99b		3.06±	0.59b		9.08±	0.66b		87.86±	1.25a
		A1B2C2		16.02±		0.22b			1.00±		0.38a			1.58±		0.36a			13.43±		0.52b		6.24±	2.27ab		9.87±	2.10b		83.89±	4.37a
		A2B1C1		11.64±		1.56c			0.44±		0.07b			0.96±		0.27c			10.24±		1.76c		3.75±	0.07b		8.49±	3.45b		87.76±	3.38a
		A2B1C2		14.74±		0.86b			0.77±		0.07ab			1.50±		0.09ab			12.48±		0.71b		5.19±	0.14b		10.17±	0.01ab		84.64±	0.13a
		A2B2C1		11.54±		1.06c			0.34±		0.01b			1.26±		0.05b			9.94±		1.09c		2.98±	0.14b		10.95±	1.44ab		86.07±	1.58a
		A2B2C2		12.25±		0.56c			0.95±		0.08a			1.13±		0.18c			10.17±		0.30c		7.74±	0.29a		9.22±	1.08b		83.04±	1.37a
		$η A 2$		0.697					0.004					0.402					0.675				0.297			0.000			0.073
		$η B 2$		0.179					0.167					0.005					0.221				0.387			0.007			0.130
		$η C 2$		0.771					0.815					0.356					0.698				0.801			0.128			0.219
		$η A × B × C 2$		0.145					0.005					0.416					0.233				0.024			0.462			0.280
打顶期		A1		73.50±		5.79a			13.34±		2.17a			13.87±		1.68a			46.29±		1.97a		18.08±	1.81a		18.91±	2.25a		63.01±	3.18a
Topping		A2		73.43±		3.90a			15.46±		0.73a			13.85±		1.46a			44.12±		2.56a		21.08±	1.99a		18.84±	1.44a		60.09±	1.29a
stage		B1		75.88±		1.13a			14.77±		1.83a			14.38±		1.03a			46.73±		3.83a		19.44±	1.71a		19.00±	1.53a		61.56±	2.36a
		B2		71.05±		3.03b			14.03±		2.03a			13.34±		1.80a			43.69±		2.26b		19.72±	2.54a		18.74±	2.18a		61.54±	3.33a
		C1		70.25±		2.06b			13.24±		1.83b			13.04±		1.51a			43.97±		2.03b		18.85±	2.59a		18.53±	1.64a		62.62±	3.16a
		C2		76.69±		4.57a			15.56±		1.16a			14.68±		1.07a			46.45±		4.17a		20.31±	1.23a		19.21±	2.04a		60.48±	2.01a
		A1B1C1		70.57±		0.12b			12.00±		0.05b			13.48±		0.27a			45.09±		0.35b		17.00±	0.04b		19.11±	0.42ab		63.89±	0.38ab
		A1B1C2		82.61±		2.49a			16.26±		0.03a			14.32±		2.10a			52.03±		0.36a		19.69±	0.56ab		17.30±	2.02b		63.00±	1.46ab
		A1B2C1		69.44±		1.71b			11.15±		0.07b			12.03±		0.91a			46.26±		0.73b		16.06±	0.29b		17.31±	0.88b		66.63±	0.59a
		A1B2C2		71.39±		0.89b			13.96±		1.33ab			15.64±		0.75a			41.79±		0.31c		19.55±	1.62ab		21.92±	1.33a		58.53±	0.29b
		A2B1C1		72.92±		1.00b			14.88±		0.25ab			15.06±		0.40a			42.99±		1.15c		20.40±	0.07a		20.66±	0.83a		58.94±	0.76b
		A2B1C2		77.43±		2.73ab			15.96±		0.86ab			14.67±		0.24a			46.80±		3.35b		20.65±	1.84a		18.95±	0.36ab		60.40±	2.20ab
		A2B2C1		68.06±		0.70b			14.93±		0.65ab			11.60±		0.39a			41.53±		0.34c		21.93±	0.73a		17.05±	0.40b		61.02±	1.13ab
		A2B2C2		75.31±		0.70ab			16.07±		0.19a			14.07±		0.33a			45.17±		0.84b		21.34±	0.05a		18.68±	0.62ab		59.98±	0.57b
		$η A 2$		0.001					0.855					0.000					0.568				0.839			0.003			0.779
		$η B 2$		0.828					0.423					0.409					0.721				0.044			0.033			0.000
		$η C 2$		0.895					0.877					0.627					0.633				0.551			0.187			0.654
		$η A × B × C 2$		0.679					0.157					0.000					0.689				0.088			0.226			0.363
圆顶期	A1		88.82±		7.00a			17.85±		1.10a			20.03±		3.95a			50.94±		2.92a			20.15±	1.33a		22.39±	2.73a		57.46±	2.33a
Round	A2		86.67±		4.05a			17.48±		1.48a			19.05±		2.43a			50.13±		3.65a			20.21±	1.97a		21.99±	2.66a		57.80±	2.21a
top stage	B1		91.78±		4.78a			18.08±		0.83a			20.71±		1.55a			52.99±		1.97a			19.72±	0.94a		22.47±	2.90a		57.81±	2.36a
	B2		83.71±		2.81b			17.25±		1.55a			18.38±		1.51b			48.08±		2.16b			20.64±	2.08a		21.91±	2.46a		57.45±	2.16a
	C1		87.98±		3.80a			17.34±		1.44a			20.13±		2.07a			50.51±		2.55a			19.73±	1.74a		22.87±	2.01a		57.41±	1.08a
	C2		87.51±		7.31a			18.00±		1.08a			18.96±		4.12a			50.56±		3.97a			20.64±	1.48a		21.51±	3.09a		57.85±	3.01a
	A1B1C1		90.43±		1.91b			17.63±		0.67ab			21.23±		1.09ab			51.57±		0.15ab			19.50±	0.32a		23.46±	0.71ab		57.04±	1.04ab
	A1B1C2		98.44±		4.25a			18.77±		0.33a			25.48±		0.31a			54.19±		4.23a			19.08±	0.48ab		25.91±	1.44a		55.00±	1.92b
	A1B2C1		84.06±		0.94cd			18.66±		0.17a			17.35±		0.11b			48.05±		1.00b			22.20±	0.45a		20.64±	0.10ab		57.15±	0.56ab
	A1B2C2		82.37±		1.01d			16.34±		0.48ab			16.08±		0.01b			49.95±		0.53b			19.83±	0.34ab		19.52±	0.24b		60.64±	0.10a
	A2B1C1		90.95±		2.12b			17.60±		1.44ab			19.82±		0.04ab			53.54±		0.64ab			19.34±	1.13ab		21.79±	0.47ab		58.87±	0.67ab
	A2B1C2		87.30±		0.40c			18.32±		0.46a			16.33±		0.99b			52.65±		1.05ab			20.98±	0.43ab		18.70±	1.05b		60.32±	1.48a
	A2B2C1		86.48±		5.20c			15.46±		1.00b			22.12±		1.68ab			48.90±		2.52b			17.87±	0.09b		25.57±	0.40a		56.56±	0.49ab
	A2B2C2		81.93±		0.79d			18.55±		0.27a			17.95±		0.57b			45.44±		1.63b			22.64±	0.55a		21.91±	0.9ab		55.45±	1.45b
	$η A 2$		0.250					0.116					0.415					0.080					0.005			0.112			0.044
	$η B 2$		0.823					0.398					0.801					0.764					0.577			0.201			0.048
	$η C 2$		0.016					0.293					0.503					0.000					0.576			0.598			0.074
	$η A × B × C 2$		0.258					0.671					0.519					0.029					0.726			0.314			0.619

Tab.2 Effects of the diameter of seedling tray,microbial agent and transplanting leaf age on N accumulation and distribution of flue-cured tobacco

取样时期 Sampling period		处理 Treatments		总氮量/(kg/hm²) Total N			氮积累量/(kg/hm²) N accumulation															氮分配比例/% N distribution
取样时期 Sampling period		处理 Treatments		总氮量/(kg/hm²) Total N			根Root					茎Stem					叶Leaf					根Root			茎Stem			叶Leaf
团棵期		A1		15.23±		3.03a			0.64±		0.31a			1.43±		0.20a			13.16±		2.80a		4.10±	1.65a		9.66±	1.88a		86.24±	2.72a
Resettling		A2		12.54±		1.61b			0.62±		0.27a			1.21±		0.25a			10.71±		1.38b		4.92±	1.94a		9.71±	1.78a		85.38±	2.40a
stage		B1		14.30±		3.43a			0.58±		0.20a			1.33±		0.27a			12.39±		3.14a		4.01±	0.82a		9.59±	2.21a		86.40±	2.26a
		B2		13.47±		1.94a			0.68±		0.35a			1.31±		0.23a			11.48±		1.69a		5.01±	2.37a		9.78±	1.33a		85.21±	2.76a
		C1		12.26±		1.69b			0.40±		0.06b			1.22±		0.21a			10.64±		1.63b		3.26±	0.39a		10.12±	2.10a		86.62±	2.04a
		C2		15.51±		2.65a			0.86±		0.20a			1.42±		0.24a			13.23±		2.59a		5.76±	1.77a		9.25±	1.35a		84.99±	2.81a
		A1B1C1		11.79±		2.58c			0.38±		0.09b			1.40±		0.20ab			10.01±		2.30c		3.24±	0.03b		11.97±	0.92a		84.80±	0.90a
		A1B1C2		19.03±		0.59a			0.74±		0.17ab			1.47±		0.04ab			16.82±		0.38a		3.88±	0.77b		7.72±	0.04c		88.41±	0.73a
		A1B2C1		14.08±		0.93b			0.43±		0.05ab			1.28±		0.01b			12.38±		0.99b		3.06±	0.59b		9.08±	0.66b		87.86±	1.25a
		A1B2C2		16.02±		0.22b			1.00±		0.38a			1.58±		0.36a			13.43±		0.52b		6.24±	2.27ab		9.87±	2.10b		83.89±	4.37a
		A2B1C1		11.64±		1.56c			0.44±		0.07b			0.96±		0.27c			10.24±		1.76c		3.75±	0.07b		8.49±	3.45b		87.76±	3.38a
		A2B1C2		14.74±		0.86b			0.77±		0.07ab			1.50±		0.09ab			12.48±		0.71b		5.19±	0.14b		10.17±	0.01ab		84.64±	0.13a
		A2B2C1		11.54±		1.06c			0.34±		0.01b			1.26±		0.05b			9.94±		1.09c		2.98±	0.14b		10.95±	1.44ab		86.07±	1.58a
		A2B2C2		12.25±		0.56c			0.95±		0.08a			1.13±		0.18c			10.17±		0.30c		7.74±	0.29a		9.22±	1.08b		83.04±	1.37a
		$η A 2$		0.697					0.004					0.402					0.675				0.297			0.000			0.073
		$η B 2$		0.179					0.167					0.005					0.221				0.387			0.007			0.130
		$η C 2$		0.771					0.815					0.356					0.698				0.801			0.128			0.219
		$η A × B × C 2$		0.145					0.005					0.416					0.233				0.024			0.462			0.280
打顶期		A1		73.50±		5.79a			13.34±		2.17a			13.87±		1.68a			46.29±		1.97a		18.08±	1.81a		18.91±	2.25a		63.01±	3.18a
Topping		A2		73.43±		3.90a			15.46±		0.73a			13.85±		1.46a			44.12±		2.56a		21.08±	1.99a		18.84±	1.44a		60.09±	1.29a
stage		B1		75.88±		1.13a			14.77±		1.83a			14.38±		1.03a			46.73±		3.83a		19.44±	1.71a		19.00±	1.53a		61.56±	2.36a
		B2		71.05±		3.03b			14.03±		2.03a			13.34±		1.80a			43.69±		2.26b		19.72±	2.54a		18.74±	2.18a		61.54±	3.33a
		C1		70.25±		2.06b			13.24±		1.83b			13.04±		1.51a			43.97±		2.03b		18.85±	2.59a		18.53±	1.64a		62.62±	3.16a
		C2		76.69±		4.57a			15.56±		1.16a			14.68±		1.07a			46.45±		4.17a		20.31±	1.23a		19.21±	2.04a		60.48±	2.01a
		A1B1C1		70.57±		0.12b			12.00±		0.05b			13.48±		0.27a			45.09±		0.35b		17.00±	0.04b		19.11±	0.42ab		63.89±	0.38ab
		A1B1C2		82.61±		2.49a			16.26±		0.03a			14.32±		2.10a			52.03±		0.36a		19.69±	0.56ab		17.30±	2.02b		63.00±	1.46ab
		A1B2C1		69.44±		1.71b			11.15±		0.07b			12.03±		0.91a			46.26±		0.73b		16.06±	0.29b		17.31±	0.88b		66.63±	0.59a
		A1B2C2		71.39±		0.89b			13.96±		1.33ab			15.64±		0.75a			41.79±		0.31c		19.55±	1.62ab		21.92±	1.33a		58.53±	0.29b
		A2B1C1		72.92±		1.00b			14.88±		0.25ab			15.06±		0.40a			42.99±		1.15c		20.40±	0.07a		20.66±	0.83a		58.94±	0.76b
		A2B1C2		77.43±		2.73ab			15.96±		0.86ab			14.67±		0.24a			46.80±		3.35b		20.65±	1.84a		18.95±	0.36ab		60.40±	2.20ab
		A2B2C1		68.06±		0.70b			14.93±		0.65ab			11.60±		0.39a			41.53±		0.34c		21.93±	0.73a		17.05±	0.40b		61.02±	1.13ab
		A2B2C2		75.31±		0.70ab			16.07±		0.19a			14.07±		0.33a			45.17±		0.84b		21.34±	0.05a		18.68±	0.62ab		59.98±	0.57b
		$η A 2$		0.001					0.855					0.000					0.568				0.839			0.003			0.779
		$η B 2$		0.828					0.423					0.409					0.721				0.044			0.033			0.000
		$η C 2$		0.895					0.877					0.627					0.633				0.551			0.187			0.654
		$η A × B × C 2$		0.679					0.157					0.000					0.689				0.088			0.226			0.363
圆顶期	A1		88.82±		7.00a			17.85±		1.10a			20.03±		3.95a			50.94±		2.92a			20.15±	1.33a		22.39±	2.73a		57.46±	2.33a
Round	A2		86.67±		4.05a			17.48±		1.48a			19.05±		2.43a			50.13±		3.65a			20.21±	1.97a		21.99±	2.66a		57.80±	2.21a
top stage	B1		91.78±		4.78a			18.08±		0.83a			20.71±		1.55a			52.99±		1.97a			19.72±	0.94a		22.47±	2.90a		57.81±	2.36a
	B2		83.71±		2.81b			17.25±		1.55a			18.38±		1.51b			48.08±		2.16b			20.64±	2.08a		21.91±	2.46a		57.45±	2.16a
	C1		87.98±		3.80a			17.34±		1.44a			20.13±		2.07a			50.51±		2.55a			19.73±	1.74a		22.87±	2.01a		57.41±	1.08a
	C2		87.51±		7.31a			18.00±		1.08a			18.96±		4.12a			50.56±		3.97a			20.64±	1.48a		21.51±	3.09a		57.85±	3.01a
	A1B1C1		90.43±		1.91b			17.63±		0.67ab			21.23±		1.09ab			51.57±		0.15ab			19.50±	0.32a		23.46±	0.71ab		57.04±	1.04ab
	A1B1C2		98.44±		4.25a			18.77±		0.33a			25.48±		0.31a			54.19±		4.23a			19.08±	0.48ab		25.91±	1.44a		55.00±	1.92b
	A1B2C1		84.06±		0.94cd			18.66±		0.17a			17.35±		0.11b			48.05±		1.00b			22.20±	0.45a		20.64±	0.10ab		57.15±	0.56ab
	A1B2C2		82.37±		1.01d			16.34±		0.48ab			16.08±		0.01b			49.95±		0.53b			19.83±	0.34ab		19.52±	0.24b		60.64±	0.10a
	A2B1C1		90.95±		2.12b			17.60±		1.44ab			19.82±		0.04ab			53.54±		0.64ab			19.34±	1.13ab		21.79±	0.47ab		58.87±	0.67ab
	A2B1C2		87.30±		0.40c			18.32±		0.46a			16.33±		0.99b			52.65±		1.05ab			20.98±	0.43ab		18.70±	1.05b		60.32±	1.48a
	A2B2C1		86.48±		5.20c			15.46±		1.00b			22.12±		1.68ab			48.90±		2.52b			17.87±	0.09b		25.57±	0.40a		56.56±	0.49ab
	A2B2C2		81.93±		0.79d			18.55±		0.27a			17.95±		0.57b			45.44±		1.63b			22.64±	0.55a		21.91±	0.9ab		55.45±	1.45b
	$η A 2$		0.250					0.116					0.415					0.080					0.005			0.112			0.044
	$η B 2$		0.823					0.398					0.801					0.764					0.577			0.201			0.048
	$η C 2$		0.016					0.293					0.503					0.000					0.576			0.598			0.074
	$η A × B × C 2$		0.258					0.671					0.519					0.029					0.726			0.314			0.619

Tab.3 Effects of the diameter of seedling tray,microbial agent and transplanting leaf age on P accumulation and distribution of flue-cured tobacco

取样时期 Sampling period		处理 Treatments	总磷量/(kg/hm²) Total P				磷积累量/(kg/hm²) P accumulation														磷分配比例/% P distribution
取样时期 Sampling period		处理 Treatments	总磷量/(kg/hm²) Total P				根Root					茎Stem				叶Leaf					根Root				茎Stem				叶Leaf
团棵期		A1	1.14±		0.10a			0.07±		0.02a		0.13±		0.02a		0.94±		0.09a			6.24±	1.42a			11.51±		1.43a		82.25±		1.87a
Resettling		A2	1.08±		0.10a			0.07±		0.01a		0.12±		0.02a		0.89±		0.09a			6.60±	1.22a			10.82±		1.80a		82.58±		2.87a
stage		B1	1.15±		0.11a			0.07±		0.01a		0.13±		0.02a		0.95±		0.11a			6.21±	1.16a			11.31±		1.75a		82.48±		2.55a
		B2	1.06±		0.06a			0.07±		0.02a		0.12±		0.02a		0.87±		0.05a			6.64±	1.46a			11.02±		1.56a		82.35±		2.30a
		C1	1.06±		0.07a			0.06±		0.01a		0.12±		0.02a		0.88±		0.08a			5.79±	1.20a			10.91±		2.12a		83.29±		2.76a
		C2	1.16±		0.10a			0.08±		0.01a		0.13±		0.01a		0.95±		0.09a			7.05±	1.11a			11.41±		0.94a		81.54±		1.56a
	A1B1C1			1.09±		0.06a			0.06±		0.01a		0.14±		0.01a		0.89±		0.06a	5.83±			1.47a	12.86±		0.36a		81.31±		1.11a
	A1B1C2			1.28±		0.10a			0.08±		0.01a		0.14±		0.01a		1.06±		0.09a	6.48±			0.15a	10.90±		0.42a		82.62±		0.58a
	A1B2C1			1.06±		0.05a			0.06±		0.02a		0.11±		0.02a		0.89±		0.06a	5.45±			1.23a	10.35±		2.54a		84.20±		1.31a
	A1B2C2			1.12±		0.02a			0.08±		0.03a		0.13±		0.01a		0.90±		0.05a	7.22±			2.60a	11.93±		0.16a		80.85±		2.76a
	A2B1C1			1.06±		0.11a			0.06±		0.02a		0.10±		0.03a		0.89±		0.15a	5.72±			2.33a	10.10±		3.54a		84.18±		5.88a
	A2B1C2			1.19±		0.09a			0.08±		0.01a		0.14±		0.01a		0.97±		0.07a	6.80±			0.36a	11.38±		0.63a		81.82±		0.27a
	A2B2C1			1.01±		0.12a			0.06±		0.01a		0.11±		0.03a		0.84±		0.08a	6.18±			0.64a	10.34±		1.50a		83.48±		2.14a
	A2B2C2			1.06±		0.01a			0.08±		0.01a		0.12±		0.02a		0.85±		0.02a	7.71±			0.04a	11.44±		2.12a		80.85±		2.16a
	$η A 2$			0.213					0.000				0.225				0.130			0.030				0.068				0.008
	$η B 2$			0.404					0.001				0.194				0.319			0.044				0.013				0.001
	$η C 2$			0.472					0.482				0.308				0.258			0.278				0.037				0.184
	$η A × B × C 2$			0.007					0.005				0.137				0.033			0.007				0.116				0.081
打顶期	A1			8.17±		0.80a			1.55±		0.25a		1.87±		0.25a		4.75±		0.35a	18.90±			1.52a	22.84±		1.29a		58.26±		1.94a
Topping	A2			8.43±		0.43a			1.65±		0.20a		2.00±		0.08a		4.79±		0.26a	19.48±			1.78a	23.72±		0.76a		56.80±		1.78a
stage	B1			8.58±		0.56a			1.67±		0.17a		1.97±		0.16a		4.93±		0.28a	19.48±			1.20a	23.00±		0.59a		57.52±		1.49a
	B2			8.02±		0.61a			1.52±		0.25a		1.89±		0.22a		4.61±		0.23a	18.90±			2.01a	23.56±		1.47a		57.54±		2.43a
	C1			7.85±		0.50b			1.43±		0.17b		1.81±		0.17b		4.61±		0.22b	18.20±			1.26a	23.01±		1.27a		58.80±		1.50a
	C2			8.75±		0.39a			1.76±		0.12a		2.06±		0.11a		4.92±		0.30a	20.19±			1.36a	23.55±		0.94a		56.26±		1.49a
	A1B1C1			7.86±		0.03b			1.44±		0.01b		1.79±		0.04ab		4.64±		0.01a	18.30±			0.05a	22.72±		0.47a		58.98±		0.43a
	A1B1C2			9.14±		0.61a			1.81±		0.11a		2.10±		0.24a		5.23±		0.27a	19.82±			0.19a	22.92±		1.06a		57.25±		0.88a
	A1B2C1			7.21±		0.16b			1.25±		0.02b		1.59±		0.19b		4.37±		0.01a	17.32±			0.69a	22.04±		2.12a		60.64±		1.42a
	A1B2C2			8.46±		0.01ab			1.71±		0.19ab		2.00±		0.15a		4.75±		0.05a	20.16±			2.25a	23.67±		1.75a		56.17±		0.51a
	A2B1C1			8.49±		0.21ab			1.69±		0.02ab		1.93±		0.04a		4.87±		0.19a	19.85±			0.68a	22.76±		0.13a		57.38±		0.81a
	A2B1C2			8.82±		0.09ab			1.76±		0.20ab		2.08±		0.01a		4.98±		0.29a	19.96±			2.42a	23.60±		0.25a		56.45±		2.67a
	A2B2C1			7.84±		0.19b			1.36±		0.06b		1.92±		0.06a		4.56±		0.20a	17.32±			1.25a	24.50±		0.13a		58.19±		1.11a
	A2B2C2			8.58±		0.39ab			1.78±		0.01ab		2.06±		0.02a		4.74±		0.36a	20.81±			0.89a	24.01±		0.86a		55.18±		1.74a
	$η A 2$			0.305					0.287				0.354				0.018			0.088				0.240				0.359
	$η B 2$			0.661					0.501				0.181				0.538			0.087				0.113				0.000
	$η C 2$			0.836					0.831				0.679				0.522			0.526				0.109				0.627
	$η A × B × C 2$			0.069					0.165				0.025				0.050			0.070				0.162				0.007
圆顶期	A1			14.71±		1.49a			2.51±		0.42a		3.75±		0.35a		8.45±		1.03a	17.03±			2.06a	25.55±		2.00a		57.41±		2.47a
Round	A2			12.99±		0.59b			2.12±		0.40b		3.37±		0.18a		7.50±		0.39a	16.28±			2.59a	25.94±		1.09a		57.78±		2.86a
top stage	B1			14.17±		1.36a			2.47±		0.38a		3.75±		0.32a		7.96±		0.91a	17.42±			2.24a	26.49±		1.40a		56.09±		1.92a
	B2			13.53±		1.48a			2.17±		0.47b		3.37±		0.22a		7.99±		0.96a	15.90±			2.22a	25.01±		1.43a		59.10±		2.35a
	C1			13.75±		1.47a			2.23±		0.42a		3.58±		0.28a		7.94±		1.03a	16.14±			1.94a	26.17±		2.06a		57.69±		2.60a
	C2			13.95±		1.45a			2.40±		0.48a		3.53±		0.39a		8.01±		0.83a	17.17±			2.63a	25.33±		0.77a		57.50±		2.75a
	A1B1C1			13.66±		0.71b			2.06±		0.05ab		3.83±		0.28a		7.76±		0.38b	15.10±			0.43b	28.05±		0.61a		56.85±		0.18ab
	A1B1C2			16.22±		0.65a			2.89±		0.33a		4.15±		0.14a		9.17±		0.84a	17.90±			2.76ab	25.58±		0.16a		56.52±		2.92ab
	A1B2C1			15.60±		1.62ab			2.85±		0.11a		3.70±		0.04a		9.05±		1.77a	18.39±			2.59ab	23.87±		2.75a		57.74±		5.34ab
	A1B2C2			13.37±		0.03b			2.24±		0.19ab		3.30±		0.09a		7.83±		0.13b	16.74±			1.49ab	24.71±		0.63a		58.55±		0.86ab
	A2B1C1			13.41±		0.85b			2.23±		0.04ab		3.60±		0.01a		7.58±		0.81b	16.65±			0.74ab	26.88±		1.74a		56.47±		2.48ab
	A2B1C2			13.40±		0.13b			2.68±		0.11ab		3.41±		0.12a		7.31±		0.35b	20.02±			0.98a	25.45±		1.12a		54.53±		2.10b
	A2B2C1			12.35±		0.36b			1.78±		0.10b		3.19±		0.11a		7.37±		0.15b	14.42±			0.40b	25.87±		0.14a		59.71±		0.54a
	A2B2C2			12.79±		0.10b			1.80±		0.07b		3.27±		0.13a		7.73±		0.16b	14.04±			0.43b	25.57±		1.22a		60.39±		0.79a
	$η A 2$			0.728					0.761				0.789				0.431			0.108				0.041				0.011
	$η B 2$			0.272					0.657				0.789				0.001			0.333				0.384				0.423
	$η C 2$			0.032					0.387				0.059				0.004			0.187				0.167				0.003
	$η A × B × C 2$			0.609					0.573				0.609				0.356			0.006				0.078				0.011

Tab.3 Effects of the diameter of seedling tray,microbial agent and transplanting leaf age on P accumulation and distribution of flue-cured tobacco

取样时期 Sampling period		处理 Treatments	总磷量/(kg/hm²) Total P				磷积累量/(kg/hm²) P accumulation														磷分配比例/% P distribution
取样时期 Sampling period		处理 Treatments	总磷量/(kg/hm²) Total P				根Root					茎Stem				叶Leaf					根Root				茎Stem				叶Leaf
团棵期		A1	1.14±		0.10a			0.07±		0.02a		0.13±		0.02a		0.94±		0.09a			6.24±	1.42a			11.51±		1.43a		82.25±		1.87a
Resettling		A2	1.08±		0.10a			0.07±		0.01a		0.12±		0.02a		0.89±		0.09a			6.60±	1.22a			10.82±		1.80a		82.58±		2.87a
stage		B1	1.15±		0.11a			0.07±		0.01a		0.13±		0.02a		0.95±		0.11a			6.21±	1.16a			11.31±		1.75a		82.48±		2.55a
		B2	1.06±		0.06a			0.07±		0.02a		0.12±		0.02a		0.87±		0.05a			6.64±	1.46a			11.02±		1.56a		82.35±		2.30a
		C1	1.06±		0.07a			0.06±		0.01a		0.12±		0.02a		0.88±		0.08a			5.79±	1.20a			10.91±		2.12a		83.29±		2.76a
		C2	1.16±		0.10a			0.08±		0.01a		0.13±		0.01a		0.95±		0.09a			7.05±	1.11a			11.41±		0.94a		81.54±		1.56a
	A1B1C1			1.09±		0.06a			0.06±		0.01a		0.14±		0.01a		0.89±		0.06a	5.83±			1.47a	12.86±		0.36a		81.31±		1.11a
	A1B1C2			1.28±		0.10a			0.08±		0.01a		0.14±		0.01a		1.06±		0.09a	6.48±			0.15a	10.90±		0.42a		82.62±		0.58a
	A1B2C1			1.06±		0.05a			0.06±		0.02a		0.11±		0.02a		0.89±		0.06a	5.45±			1.23a	10.35±		2.54a		84.20±		1.31a
	A1B2C2			1.12±		0.02a			0.08±		0.03a		0.13±		0.01a		0.90±		0.05a	7.22±			2.60a	11.93±		0.16a		80.85±		2.76a
	A2B1C1			1.06±		0.11a			0.06±		0.02a		0.10±		0.03a		0.89±		0.15a	5.72±			2.33a	10.10±		3.54a		84.18±		5.88a
	A2B1C2			1.19±		0.09a			0.08±		0.01a		0.14±		0.01a		0.97±		0.07a	6.80±			0.36a	11.38±		0.63a		81.82±		0.27a
	A2B2C1			1.01±		0.12a			0.06±		0.01a		0.11±		0.03a		0.84±		0.08a	6.18±			0.64a	10.34±		1.50a		83.48±		2.14a
	A2B2C2			1.06±		0.01a			0.08±		0.01a		0.12±		0.02a		0.85±		0.02a	7.71±			0.04a	11.44±		2.12a		80.85±		2.16a
	$η A 2$			0.213					0.000				0.225				0.130			0.030				0.068				0.008
	$η B 2$			0.404					0.001				0.194				0.319			0.044				0.013				0.001
	$η C 2$			0.472					0.482				0.308				0.258			0.278				0.037				0.184
	$η A × B × C 2$			0.007					0.005				0.137				0.033			0.007				0.116				0.081
打顶期	A1			8.17±		0.80a			1.55±		0.25a		1.87±		0.25a		4.75±		0.35a	18.90±			1.52a	22.84±		1.29a		58.26±		1.94a
Topping	A2			8.43±		0.43a			1.65±		0.20a		2.00±		0.08a		4.79±		0.26a	19.48±			1.78a	23.72±		0.76a		56.80±		1.78a
stage	B1			8.58±		0.56a			1.67±		0.17a		1.97±		0.16a		4.93±		0.28a	19.48±			1.20a	23.00±		0.59a		57.52±		1.49a
	B2			8.02±		0.61a			1.52±		0.25a		1.89±		0.22a		4.61±		0.23a	18.90±			2.01a	23.56±		1.47a		57.54±		2.43a
	C1			7.85±		0.50b			1.43±		0.17b		1.81±		0.17b		4.61±		0.22b	18.20±			1.26a	23.01±		1.27a		58.80±		1.50a
	C2			8.75±		0.39a			1.76±		0.12a		2.06±		0.11a		4.92±		0.30a	20.19±			1.36a	23.55±		0.94a		56.26±		1.49a
	A1B1C1			7.86±		0.03b			1.44±		0.01b		1.79±		0.04ab		4.64±		0.01a	18.30±			0.05a	22.72±		0.47a		58.98±		0.43a
	A1B1C2			9.14±		0.61a			1.81±		0.11a		2.10±		0.24a		5.23±		0.27a	19.82±			0.19a	22.92±		1.06a		57.25±		0.88a
	A1B2C1			7.21±		0.16b			1.25±		0.02b		1.59±		0.19b		4.37±		0.01a	17.32±			0.69a	22.04±		2.12a		60.64±		1.42a
	A1B2C2			8.46±		0.01ab			1.71±		0.19ab		2.00±		0.15a		4.75±		0.05a	20.16±			2.25a	23.67±		1.75a		56.17±		0.51a
	A2B1C1			8.49±		0.21ab			1.69±		0.02ab		1.93±		0.04a		4.87±		0.19a	19.85±			0.68a	22.76±		0.13a		57.38±		0.81a
	A2B1C2			8.82±		0.09ab			1.76±		0.20ab		2.08±		0.01a		4.98±		0.29a	19.96±			2.42a	23.60±		0.25a		56.45±		2.67a
	A2B2C1			7.84±		0.19b			1.36±		0.06b		1.92±		0.06a		4.56±		0.20a	17.32±			1.25a	24.50±		0.13a		58.19±		1.11a
	A2B2C2			8.58±		0.39ab			1.78±		0.01ab		2.06±		0.02a		4.74±		0.36a	20.81±			0.89a	24.01±		0.86a		55.18±		1.74a
	$η A 2$			0.305					0.287				0.354				0.018			0.088				0.240				0.359
	$η B 2$			0.661					0.501				0.181				0.538			0.087				0.113				0.000
	$η C 2$			0.836					0.831				0.679				0.522			0.526				0.109				0.627
	$η A × B × C 2$			0.069					0.165				0.025				0.050			0.070				0.162				0.007
圆顶期	A1			14.71±		1.49a			2.51±		0.42a		3.75±		0.35a		8.45±		1.03a	17.03±			2.06a	25.55±		2.00a		57.41±		2.47a
Round	A2			12.99±		0.59b			2.12±		0.40b		3.37±		0.18a		7.50±		0.39a	16.28±			2.59a	25.94±		1.09a		57.78±		2.86a
top stage	B1			14.17±		1.36a			2.47±		0.38a		3.75±		0.32a		7.96±		0.91a	17.42±			2.24a	26.49±		1.40a		56.09±		1.92a
	B2			13.53±		1.48a			2.17±		0.47b		3.37±		0.22a		7.99±		0.96a	15.90±			2.22a	25.01±		1.43a		59.10±		2.35a
	C1			13.75±		1.47a			2.23±		0.42a		3.58±		0.28a		7.94±		1.03a	16.14±			1.94a	26.17±		2.06a		57.69±		2.60a
	C2			13.95±		1.45a			2.40±		0.48a		3.53±		0.39a		8.01±		0.83a	17.17±			2.63a	25.33±		0.77a		57.50±		2.75a
	A1B1C1			13.66±		0.71b			2.06±		0.05ab		3.83±		0.28a		7.76±		0.38b	15.10±			0.43b	28.05±		0.61a		56.85±		0.18ab
	A1B1C2			16.22±		0.65a			2.89±		0.33a		4.15±		0.14a		9.17±		0.84a	17.90±			2.76ab	25.58±		0.16a		56.52±		2.92ab
	A1B2C1			15.60±		1.62ab			2.85±		0.11a		3.70±		0.04a		9.05±		1.77a	18.39±			2.59ab	23.87±		2.75a		57.74±		5.34ab
	A1B2C2			13.37±		0.03b			2.24±		0.19ab		3.30±		0.09a		7.83±		0.13b	16.74±			1.49ab	24.71±		0.63a		58.55±		0.86ab
	A2B1C1			13.41±		0.85b			2.23±		0.04ab		3.60±		0.01a		7.58±		0.81b	16.65±			0.74ab	26.88±		1.74a		56.47±		2.48ab
	A2B1C2			13.40±		0.13b			2.68±		0.11ab		3.41±		0.12a		7.31±		0.35b	20.02±			0.98a	25.45±		1.12a		54.53±		2.10b
	A2B2C1			12.35±		0.36b			1.78±		0.10b		3.19±		0.11a		7.37±		0.15b	14.42±			0.40b	25.87±		0.14a		59.71±		0.54a
	A2B2C2			12.79±		0.10b			1.80±		0.07b		3.27±		0.13a		7.73±		0.16b	14.04±			0.43b	25.57±		1.22a		60.39±		0.79a
	$η A 2$			0.728					0.761				0.789				0.431			0.108				0.041				0.011
	$η B 2$			0.272					0.657				0.789				0.001			0.333				0.384				0.423
	$η C 2$			0.032					0.387				0.059				0.004			0.187				0.167				0.003
	$η A × B × C 2$			0.609					0.573				0.609				0.356			0.006				0.078				0.011

Tab.4 Effects of the diameter of seedling tray,microbial agent and transplanting leaf age on K accumulation and distribution of flue-cured tobacco

取样时期 Sampling period	处理 Treatments	总钾量/(kg/hm²) Total dry matter				钾积累量/(kg/hm²) K accumulation													钾分配比例/% K distribution
取样时期 Sampling period	处理 Treatments	总钾量/(kg/hm²) Total dry matter				根Root					茎Stem				叶Leaf				根Root				茎Stem					叶Leaf
团棵期	A1	6.49±		0.60a			0.29±		0.09a		0.81±		0.16a		5.38±		0.54a		4.45±		1.03a		12.59±		2.59a			82.95±	2.89a
Resettling	A2	6.08±		0.43a			0.26±		0.06a		0.76±		0.10a		5.06±		0.38a		4.34±		0.79a		12.45±		1.56a			83.20±	1.11a
stage	B1	6.42±		0.65a			0.28±		0.08a		0.80±		0.07a		5.33±		0.54a		4.35±		0.87a		12.57±		1.26a			83.08±	0.91a
	B2	6.15±		0.41a			0.27±		0.06a		0.77±		0.17a		5.11±		0.41a		4.44±		0.96a		12.48±		2.75a			83.08±	2.96a
	C1	6.03±		0.39a			0.22±		0.04b		0.75±		0.09b		5.05±		0.36a		3.72±		0.57a		12.47±		1.42a			83.81±	1.24a
	C2	6.54±		0.57a			0.33±		0.05a		0.82±		0.16a		5.39±		0.55a		5.07±		0.56a		12.58±		2.67a			82.35±	2.61a
	A1B1C1	6.15±		0.32a			0.23±		0.08b		0.76±		0.09a		5.17±		0.32ab		3.63±		1.18b		12.37±		2.03b			84.00±	0.85ab
	A1B1C2	7.34±		0.29a			0.40±		0.04a		0.84±		0.02a		6.10±		0.24a		5.46±		0.29a		11.47±		0.18b			83.08±	0.11ab
	A1B2C1	6.26±		0.60a			0.23±		0.03b		0.69±		0.12a		5.34±		0.51ab		3.72±		0.86b		10.95±		0.94c			85.33±	0.08a
	A1B2C2	6.21±		0.02a			0.31±		0.02ab		0.97±		0.24a		4.93±		0.29b		5.00±		0.37ab		15.59±		3.97a			79.40±	4.34b
	A2B1C1	5.87±		0.57a			0.23±		0.02b		0.79±		0.13a		4.85±		0.42b		3.95±		0.02b		13.41±		0.94b			82.63±	0.96ab
	A2B1C2	6.31±		0.07a			0.28±		0.01ab		0.82±		0.07a		5.21±		0.14ab		4.36±		0.01ab		13.04±		1.27b			82.59±	1.27ab
	A2B2C1	5.83±		0.11a			0.21±		0.01b		0.77±		0.07a		4.85±		0.03b		3.59±		0.11b		13.14±		0.92b			83.28±	1.03ab
	A2B2C2	6.31±		0.72a			0.34±		0.01ab		0.64±		0.05a		5.32±		0.68ab		5.46±		0.61a		10.23±		0.44c			84.32±	1.05ab
	$η A 2$	0.319					0.216				0.107				0.266				0.018				0.003					0.010
	$η B 2$	0.165					0.038				0.045				0.142				0.012				0.002					0.000
	$η C 2$	0.429					0.815				0.145				0.287				0.726				0.002					0.261
	$η A × B × C 2$	0.221					0.446				0.217				0.310				0.269				0.401					0.276
打顶期	A1	39.09±		3.59a			5.11±		0.59a		10.00±		1.22a		23.98±		2.53a		13.10±		1.35a		25.57±		1.75a			61.33±	2.76a
Topping	A2	39.87±		2.04a			5.39±		0.34a		10.24±		0.86a		24.24±		1.80a		13.53±		0.73a		25.70±		2.12a			60.77±	2.51a
stage	B1	41.40±		1.68a			5.35±		0.46a		10.55±		1.00a		25.49±		1.68a		12.93±		0.98a		25.50±		2.40a			61.57±	3.07a
	B2	37.56±		2.49b			5.15±		0.52a		9.68±		0.90a		22.73±		1.60b		13.70±		1.08a		25.76±		1.33a			60.54±	2.03a
	C1	38.48±		2.77b			4.93±		0.48b		9.52±		0.61a		24.03±		2.12a		12.82±		1.10a		24.77±		1.05a			62.41±	1.71a
	C2	40.48±		2.72a			5.57±		0.19a		10.72±		1.03a		24.20±		2.28a		13.81±		0.84a		26.49±		2.18a			59.70±	2.65a
	A1B1C1	40.60±		0.61ab			4.65±		0.10a		9.74±		0.55b		26.21±		1.06a		11.45±		0.08a		24.00±		1.71a			64.55±	1.63a
	A1B1C2	43.60±		1.25a			5.70±		0.33a		11.48±		1.20a		26.42±		0.29a		13.07±		0.39a		26.30±		2.00a			60.63±	2.39a
	A1B2C1	35.07±		0.27c			4.52±		0.13a		8.88±		0.36b		21.67±		0.50b		12.89±		0.27a		25.34±		1.23a			61.78±	0.95a
	A1B2C2	37.10±		1.67b			5.56±		0.28a		9.90±		1.24b		21.64±		0.16b		14.98±		0.07a		26.64±		2.13a			58.39±	2.20a
	A2B1C1	41.29±		0.10ab			5.57±		0.01a		10.19±		0.43ab		25.53±		0.55ab		13.49±		0.06a		24.68±		1.11a			61.83±	1.18a
	A2B1C2	40.10±		1.84ab			5.49±		0.02a		10.80±		1.30ab		23.81±		3.13ab		13.70±		0.59a		27.03±		4.49a			59.27±	5.08a
	A2B2C1	36.94±		0.65bc			4.97±		0.54a		9.26±		0.15b		22.72±		1.05b		13.46±		1.71a		25.06±		0.04a			61.48±	1.75a
	A2B2C2	41.15±		0.83ab			5.54±		0.16a		10.69±		0.53ab		24.91±		1.52ab		13.47±		0.66a		26.01±		1.81a			60.52±	2.47a
	$η A 2$	0.207					0.378				0.038				0.018				0.161				0.002					0.024
	$η B 2$	0.864					0.242				0.351				0.671				0.377				0.007					0.077
	$η C 2$	0.634					0.757				0.507				0.007				0.496				0.240					0.368
	$η A × B × C 2$	0.522					0.173				0.097				0.223				0.028				0.001					0.006
圆顶期	A1		57.31±		1.83a			5.26±		0.45a		15.11±		1.52a		36.93±		2.55a		9.20±		0.87a		26.34±		1.38a	64.46±			1.75a
Round	A2		54.87±		1.29b			5.08±		0.45a		14.13±		0.75a		35.66±		2.80a		9.25±		0.55a		25.80±		1.63a	64.95±			1.78a
top stage	B1		58.54±		3.63a			5.36±		0.46a		15.55±		1.12a		37.62±		2.98a		9.17±		0.70a		26.61±		1.82a	64.22±			1.86a
	B2		53.64±		1.42b			4.98±		0.36a		13.69±		0.40b		34.97±		1.55b		9.29±		0.75a		25.53±		0.89a	65.18±			1.54a
	C1		54.27±		2.43b			5.13±		0.41a		14.55±		1.10a		34.59±		1.71b		9.47±		0.84a		26.80±		1.30a	63.73±			1.37a
	C2		57.90±		1.93a			5.21±		0.51a		14.69±		1.48a		38.01±		2.38a		8.98±		0.46a		25.34±		1.36a	65.67±			1.52a
	A1B1C1		57.89±		0.09ab			4.98±		0.02a		15.83±		0.96ab		37.08±		0.90ab		8.60±		0.02a		27.35±		1.62a	64.05±			1.64a
	A1B1C2		62.72±		1.09a			5.71±		0.48a		16.97±		0.12a		40.04±		1.45a		9.10±		0.92a		27.06±		0.28a	63.83±			1.20a
	A1B2C1		53.24±		1.27b			5.55±		0.09a		14.05±		0.16ab		33.64±		1.03b		10.42±		0.09a		26.40±		0.33a	63.18±			0.41a
	A1B2C2		55.37±		0.06ab			4.81±		0.21a		13.60±		0.58b		36.97±		0.85ab		8.68±		0.39a		24.56±		1.07a	66.76±			1.46a
	A2B1C1		53.66±		1.08b			5.20±		0.73a		15.02±		0.35ab		33.44±		0.70b		9.68±		1.17a		28.01±		1.22a	62.31±			0.05a
	A2B1C2		59.88±		1.69ab			5.56±		0.19a		14.39±		0.57ab		39.93±		0.94a		9.29±		0.05a		24.02±		0.27a	66.69±			0.32a
	A2B2C1		52.29±		1.29b			4.80±		0.05a		13.30±		0.28b		34.18±		0.96b		9.19±		0.13a		25.44±		0.09a	65.38±			0.23a
	A2B2C2		53.64±		0.84b			4.75±		0.06a		13.80±		0.24b		35.09±		1.14b		8.86±		0.24a		25.73±		0.85a	65.41±			1.09a
	$η A 2$		0.721					0.132				0.674				0.439				0.004				0.158			0.110
	$η B 2$		0.912					0.408				0.881				0.773				0.023				0.427			0.321
	$η C 2$		0.851					0.026				0.038				0.850				0.278				0.575			0.659
	$η A × B × C 2$		0.113					0.245				0.497				0.517				0.352				0.576			0.680

Tab.4 Effects of the diameter of seedling tray,microbial agent and transplanting leaf age on K accumulation and distribution of flue-cured tobacco

取样时期 Sampling period	处理 Treatments	总钾量/(kg/hm²) Total dry matter				钾积累量/(kg/hm²) K accumulation													钾分配比例/% K distribution
取样时期 Sampling period	处理 Treatments	总钾量/(kg/hm²) Total dry matter				根Root					茎Stem				叶Leaf				根Root				茎Stem					叶Leaf
团棵期	A1	6.49±		0.60a			0.29±		0.09a		0.81±		0.16a		5.38±		0.54a		4.45±		1.03a		12.59±		2.59a			82.95±	2.89a
Resettling	A2	6.08±		0.43a			0.26±		0.06a		0.76±		0.10a		5.06±		0.38a		4.34±		0.79a		12.45±		1.56a			83.20±	1.11a
stage	B1	6.42±		0.65a			0.28±		0.08a		0.80±		0.07a		5.33±		0.54a		4.35±		0.87a		12.57±		1.26a			83.08±	0.91a
	B2	6.15±		0.41a			0.27±		0.06a		0.77±		0.17a		5.11±		0.41a		4.44±		0.96a		12.48±		2.75a			83.08±	2.96a
	C1	6.03±		0.39a			0.22±		0.04b		0.75±		0.09b		5.05±		0.36a		3.72±		0.57a		12.47±		1.42a			83.81±	1.24a
	C2	6.54±		0.57a			0.33±		0.05a		0.82±		0.16a		5.39±		0.55a		5.07±		0.56a		12.58±		2.67a			82.35±	2.61a
	A1B1C1	6.15±		0.32a			0.23±		0.08b		0.76±		0.09a		5.17±		0.32ab		3.63±		1.18b		12.37±		2.03b			84.00±	0.85ab
	A1B1C2	7.34±		0.29a			0.40±		0.04a		0.84±		0.02a		6.10±		0.24a		5.46±		0.29a		11.47±		0.18b			83.08±	0.11ab
	A1B2C1	6.26±		0.60a			0.23±		0.03b		0.69±		0.12a		5.34±		0.51ab		3.72±		0.86b		10.95±		0.94c			85.33±	0.08a
	A1B2C2	6.21±		0.02a			0.31±		0.02ab		0.97±		0.24a		4.93±		0.29b		5.00±		0.37ab		15.59±		3.97a			79.40±	4.34b
	A2B1C1	5.87±		0.57a			0.23±		0.02b		0.79±		0.13a		4.85±		0.42b		3.95±		0.02b		13.41±		0.94b			82.63±	0.96ab
	A2B1C2	6.31±		0.07a			0.28±		0.01ab		0.82±		0.07a		5.21±		0.14ab		4.36±		0.01ab		13.04±		1.27b			82.59±	1.27ab
	A2B2C1	5.83±		0.11a			0.21±		0.01b		0.77±		0.07a		4.85±		0.03b		3.59±		0.11b		13.14±		0.92b			83.28±	1.03ab
	A2B2C2	6.31±		0.72a			0.34±		0.01ab		0.64±		0.05a		5.32±		0.68ab		5.46±		0.61a		10.23±		0.44c			84.32±	1.05ab
	$η A 2$	0.319					0.216				0.107				0.266				0.018				0.003					0.010
	$η B 2$	0.165					0.038				0.045				0.142				0.012				0.002					0.000
	$η C 2$	0.429					0.815				0.145				0.287				0.726				0.002					0.261
	$η A × B × C 2$	0.221					0.446				0.217				0.310				0.269				0.401					0.276
打顶期	A1	39.09±		3.59a			5.11±		0.59a		10.00±		1.22a		23.98±		2.53a		13.10±		1.35a		25.57±		1.75a			61.33±	2.76a
Topping	A2	39.87±		2.04a			5.39±		0.34a		10.24±		0.86a		24.24±		1.80a		13.53±		0.73a		25.70±		2.12a			60.77±	2.51a
stage	B1	41.40±		1.68a			5.35±		0.46a		10.55±		1.00a		25.49±		1.68a		12.93±		0.98a		25.50±		2.40a			61.57±	3.07a
	B2	37.56±		2.49b			5.15±		0.52a		9.68±		0.90a		22.73±		1.60b		13.70±		1.08a		25.76±		1.33a			60.54±	2.03a
	C1	38.48±		2.77b			4.93±		0.48b		9.52±		0.61a		24.03±		2.12a		12.82±		1.10a		24.77±		1.05a			62.41±	1.71a
	C2	40.48±		2.72a			5.57±		0.19a		10.72±		1.03a		24.20±		2.28a		13.81±		0.84a		26.49±		2.18a			59.70±	2.65a
	A1B1C1	40.60±		0.61ab			4.65±		0.10a		9.74±		0.55b		26.21±		1.06a		11.45±		0.08a		24.00±		1.71a			64.55±	1.63a
	A1B1C2	43.60±		1.25a			5.70±		0.33a		11.48±		1.20a		26.42±		0.29a		13.07±		0.39a		26.30±		2.00a			60.63±	2.39a
	A1B2C1	35.07±		0.27c			4.52±		0.13a		8.88±		0.36b		21.67±		0.50b		12.89±		0.27a		25.34±		1.23a			61.78±	0.95a
	A1B2C2	37.10±		1.67b			5.56±		0.28a		9.90±		1.24b		21.64±		0.16b		14.98±		0.07a		26.64±		2.13a			58.39±	2.20a
	A2B1C1	41.29±		0.10ab			5.57±		0.01a		10.19±		0.43ab		25.53±		0.55ab		13.49±		0.06a		24.68±		1.11a			61.83±	1.18a
	A2B1C2	40.10±		1.84ab			5.49±		0.02a		10.80±		1.30ab		23.81±		3.13ab		13.70±		0.59a		27.03±		4.49a			59.27±	5.08a
	A2B2C1	36.94±		0.65bc			4.97±		0.54a		9.26±		0.15b		22.72±		1.05b		13.46±		1.71a		25.06±		0.04a			61.48±	1.75a
	A2B2C2	41.15±		0.83ab			5.54±		0.16a		10.69±		0.53ab		24.91±		1.52ab		13.47±		0.66a		26.01±		1.81a			60.52±	2.47a
	$η A 2$	0.207					0.378				0.038				0.018				0.161				0.002					0.024
	$η B 2$	0.864					0.242				0.351				0.671				0.377				0.007					0.077
	$η C 2$	0.634					0.757				0.507				0.007				0.496				0.240					0.368
	$η A × B × C 2$	0.522					0.173				0.097				0.223				0.028				0.001					0.006
圆顶期	A1		57.31±		1.83a			5.26±		0.45a		15.11±		1.52a		36.93±		2.55a		9.20±		0.87a		26.34±		1.38a	64.46±			1.75a
Round	A2		54.87±		1.29b			5.08±		0.45a		14.13±		0.75a		35.66±		2.80a		9.25±		0.55a		25.80±		1.63a	64.95±			1.78a
top stage	B1		58.54±		3.63a			5.36±		0.46a		15.55±		1.12a		37.62±		2.98a		9.17±		0.70a		26.61±		1.82a	64.22±			1.86a
	B2		53.64±		1.42b			4.98±		0.36a		13.69±		0.40b		34.97±		1.55b		9.29±		0.75a		25.53±		0.89a	65.18±			1.54a
	C1		54.27±		2.43b			5.13±		0.41a		14.55±		1.10a		34.59±		1.71b		9.47±		0.84a		26.80±		1.30a	63.73±			1.37a
	C2		57.90±		1.93a			5.21±		0.51a		14.69±		1.48a		38.01±		2.38a		8.98±		0.46a		25.34±		1.36a	65.67±			1.52a
	A1B1C1		57.89±		0.09ab			4.98±		0.02a		15.83±		0.96ab		37.08±		0.90ab		8.60±		0.02a		27.35±		1.62a	64.05±			1.64a
	A1B1C2		62.72±		1.09a			5.71±		0.48a		16.97±		0.12a		40.04±		1.45a		9.10±		0.92a		27.06±		0.28a	63.83±			1.20a
	A1B2C1		53.24±		1.27b			5.55±		0.09a		14.05±		0.16ab		33.64±		1.03b		10.42±		0.09a		26.40±		0.33a	63.18±			0.41a
	A1B2C2		55.37±		0.06ab			4.81±		0.21a		13.60±		0.58b		36.97±		0.85ab		8.68±		0.39a		24.56±		1.07a	66.76±			1.46a
	A2B1C1		53.66±		1.08b			5.20±		0.73a		15.02±		0.35ab		33.44±		0.70b		9.68±		1.17a		28.01±		1.22a	62.31±			0.05a
	A2B1C2		59.88±		1.69ab			5.56±		0.19a		14.39±		0.57ab		39.93±		0.94a		9.29±		0.05a		24.02±		0.27a	66.69±			0.32a
	A2B2C1		52.29±		1.29b			4.80±		0.05a		13.30±		0.28b		34.18±		0.96b		9.19±		0.13a		25.44±		0.09a	65.38±			0.23a
	A2B2C2		53.64±		0.84b			4.75±		0.06a		13.80±		0.24b		35.09±		1.14b		8.86±		0.24a		25.73±		0.85a	65.41±			1.09a
	$η A 2$		0.721					0.132				0.674				0.439				0.004				0.158			0.110
	$η B 2$		0.912					0.408				0.881				0.773				0.023				0.427			0.321
	$η C 2$		0.851					0.026				0.038				0.850				0.278				0.575			0.659
	$η A × B × C 2$		0.113					0.245				0.497				0.517				0.352				0.576			0.680

Tab.5 Effects of the diameter of seedling tray,microbial agent and transplanting leaf age on N fertilizer efficiency of flue-cured tobacco

处理 Treatments	氮肥干物质积累效率/(kg/kg) N-FDAE	氮肥烟叶利用效率/(kg/kg) N-FLAE	氮肥吸收效率/(g/kg) N-FAE	氮素烟叶生产效率/(kg/kg) N-LPE
A1	35.33±1.95a	18.77±1.66a	553.43±4.58a	33.81±2.19a
A2	33.79±1.64b	18.17±0.64a	539.99±5.23b	33.54±1.18a
B1	36.42±1.01a	19.35±1.07a	571.85±9.81a	33.72±1.85a
B2	32.70±0.92b	17.60±0.70a	521.56±7.48b	33.63±1.67a
C1	33.72±1.01b	18.14±1.20a	548.17±3.67a	32.93±1.28a
C2	35.40±1.67a	18.81±1.30a	545.24±5.54a	34.42±1.82a
A1B1C1	36.22±0.29ab	19.62±0.31a	563.43±1.88ab	34.69±1.28a
A1B1C2	39.30±1.47a	20.50±1.61a	613.35±2.47a	33.36±4.06a
A1B2C1	32.22±0.35b	16.74±0.30a	523.74±5.83bc	31.82±0.23a
A1B2C2	33.58±0.04ab	18.24±0.57a	513.19±6.31c	35.39±0.66a
A2B1C1	34.55±0.95ab	18.54±0.64a	566.69±3.22ab	32.57±0.36a
A2B1C2	35.60±0.14ab	18.73±0.23a	543.94±2.47b	34.28±0.59a
A2B2C1	31.88±1.43b	17.67±0.64a	538.82±2.41b	32.67±0.77a
A2B2C2	33.13±0.25ab	17.77±0.50a	510.50±4.93c	34.65±1.32a
$η A 2$	0.636	0.254	0.250	0.014
$η B 2$	0.911	0.742	0.823	0.002
$η C 2$	0.678	0.296	0.016	0.292
$η A × B × C 2$	0.146	0.029	0.258	0.200

Tab.5 Effects of the diameter of seedling tray,microbial agent and transplanting leaf age on N fertilizer efficiency of flue-cured tobacco

处理 Treatments	氮肥干物质积累效率/(kg/kg) N-FDAE	氮肥烟叶利用效率/(kg/kg) N-FLAE	氮肥吸收效率/(g/kg) N-FAE	氮素烟叶生产效率/(kg/kg) N-LPE
A1	35.33±1.95a	18.77±1.66a	553.43±4.58a	33.81±2.19a
A2	33.79±1.64b	18.17±0.64a	539.99±5.23b	33.54±1.18a
B1	36.42±1.01a	19.35±1.07a	571.85±9.81a	33.72±1.85a
B2	32.70±0.92b	17.60±0.70a	521.56±7.48b	33.63±1.67a
C1	33.72±1.01b	18.14±1.20a	548.17±3.67a	32.93±1.28a
C2	35.40±1.67a	18.81±1.30a	545.24±5.54a	34.42±1.82a
A1B1C1	36.22±0.29ab	19.62±0.31a	563.43±1.88ab	34.69±1.28a
A1B1C2	39.30±1.47a	20.50±1.61a	613.35±2.47a	33.36±4.06a
A1B2C1	32.22±0.35b	16.74±0.30a	523.74±5.83bc	31.82±0.23a
A1B2C2	33.58±0.04ab	18.24±0.57a	513.19±6.31c	35.39±0.66a
A2B1C1	34.55±0.95ab	18.54±0.64a	566.69±3.22ab	32.57±0.36a
A2B1C2	35.60±0.14ab	18.73±0.23a	543.94±2.47b	34.28±0.59a
A2B2C1	31.88±1.43b	17.67±0.64a	538.82±2.41b	32.67±0.77a
A2B2C2	33.13±0.25ab	17.77±0.50a	510.50±4.93c	34.65±1.32a
$η A 2$	0.636	0.254	0.250	0.014
$η B 2$	0.911	0.742	0.823	0.002
$η C 2$	0.678	0.296	0.016	0.292
$η A × B × C 2$	0.146	0.029	0.258	0.200

Tab.6 Effects of the diameter of seedling tray,microbial agent and transplanting leaf age on P fertilizer efficiency of flue-cured tobacco

处理 Treatments	磷肥干物质积累效率/(kg/kg) P-FDAE	磷肥烟叶利用效率/(kg/kg) P-FLAE	磷肥吸收效率/(g/kg) P-FAE	磷素烟叶生产效率/(kg/kg) P-LPE
A1	18.69±1.65a	12.31±1.03a	92.06±2.31a	205.50±4.84b
A2	18.09±0.64a	11.78±0.57a	81.28±3.72b	223.69±4.39a
B1	19.26±1.06a	12.69±0.70a	88.68±2.50a	219.05±3.22a
B2	17.52±0.70b	11.40±0.32a	84.66±1.24b	210.14±4.61a
C1	18.06±1.20a	11.75±0.70a	86.08±1.17a	212.96±6.81a
C2	18.72±1.29a	12.34±0.93a	87.27±1.06a	216.23±1.02a
A1B1C1	19.54±0.30a	12.62±0.10a	85.46±4.47b	230.00±1.61a
A1B1C2	20.41±1.61a	13.70±0.52a	101.48±4.09a	201.83±7.78ab
A1B2C1	16.67±0.30a	11.23±0.13a	97.65±10.1a	172.16±1.67b
A1B2C2	18.16±0.57a	11.70±0.01a	83.67±0.21b	218.00±6.21ab
A2B1C1	18.46±0.64a	12.05±0.33a	83.93±5.33b	221.09±6.46ab
A2B1C2	18.65±0.23a	12.41±0.05a	83.86±0.79b	223.27±0.71ab
A2B2C1	17.59±0.64a	11.11±0.49a	77.27±2.24b	228.59±1.72ab
A2B2C2	17.69±0.50a	11.55±0.09a	80.07±0.61b	221.82±4.60ab
$η A 2$	0.253	0.635	0.729	0.680
$η B 2$	0.742	0.911	0.272	0.337
$η C 2$	0.294	0.675	0.032	0.064
$η A × B × C 2$	0.029	0.149	0.609	0.734

Tab.6 Effects of the diameter of seedling tray,microbial agent and transplanting leaf age on P fertilizer efficiency of flue-cured tobacco

处理 Treatments	磷肥干物质积累效率/(kg/kg) P-FDAE	磷肥烟叶利用效率/(kg/kg) P-FLAE	磷肥吸收效率/(g/kg) P-FAE	磷素烟叶生产效率/(kg/kg) P-LPE
A1	18.69±1.65a	12.31±1.03a	92.06±2.31a	205.50±4.84b
A2	18.09±0.64a	11.78±0.57a	81.28±3.72b	223.69±4.39a
B1	19.26±1.06a	12.69±0.70a	88.68±2.50a	219.05±3.22a
B2	17.52±0.70b	11.40±0.32a	84.66±1.24b	210.14±4.61a
C1	18.06±1.20a	11.75±0.70a	86.08±1.17a	212.96±6.81a
C2	18.72±1.29a	12.34±0.93a	87.27±1.06a	216.23±1.02a
A1B1C1	19.54±0.30a	12.62±0.10a	85.46±4.47b	230.00±1.61a
A1B1C2	20.41±1.61a	13.70±0.52a	101.48±4.09a	201.83±7.78ab
A1B2C1	16.67±0.30a	11.23±0.13a	97.65±10.1a	172.16±1.67b
A1B2C2	18.16±0.57a	11.70±0.01a	83.67±0.21b	218.00±6.21ab
A2B1C1	18.46±0.64a	12.05±0.33a	83.93±5.33b	221.09±6.46ab
A2B1C2	18.65±0.23a	12.41±0.05a	83.86±0.79b	223.27±0.71ab
A2B2C1	17.59±0.64a	11.11±0.49a	77.27±2.24b	228.59±1.72ab
A2B2C2	17.69±0.50a	11.55±0.09a	80.07±0.61b	221.82±4.60ab
$η A 2$	0.253	0.635	0.729	0.680
$η B 2$	0.742	0.911	0.272	0.337
$η C 2$	0.294	0.675	0.032	0.064
$η A × B × C 2$	0.029	0.149	0.609	0.734

Tab.7 Effects of the diameter of seedling tray,microbial agent and transplanting leaf age on K fertilizer efficiency of flue-cured tobacco

处理 Treatments	钾肥干物质积累效率/(kg/kg) K-FDAE	钾肥烟叶利用效率/(kg/kg) K-FLAE	钾肥吸收效率/(g/kg) K-FAE	钾素烟叶生产效率/(kg/kg) K-LPE
A1	35.48±1.97a	6.51±0.58a	124.44±8.31a	52.31±2.05a
A2	33.94±1.65b	6.31±0.22a	119.14±7.15b	53.02±2.24a
B1	36.58±2.02a	6.71±0.37a	127.11±7.88a	52.89±2.60a
B2	32.84±0.92b	6.11±0.25a	116.47±3.07b	52.44±1.62a
C1	33.86±2.02b	6.29±0.42a	117.85±5.28b	53.39±2.08a
C2	35.56±2.68a	6.53±0.45a	125.74±8.53a	51.94±2.00a
A1B1C1	36.38±0.29ab	6.81±0.11a	125.72±0.19ab	54.16±0.93a
A1B1C2	39.48±1.48a	7.12±0.56a	136.19±2.36a	52.20±3.21a
A1B2C1	32.36±0.35b	5.81±0.11a	115.62±2.76b	50.24±0.28a
A1B2C2	33.72±0.03b	6.33±0.20a	120.25±0.12ab	52.64±1.58a
A2B1C1	34.70±0.96ab	6.44±0.22a	116.53±2.34b	55.21±0.78a
A2B1C2	35.76±0.15ab	6.50±0.08a	130.03±3.67ab	50.01±2.04a
A2B2C1	32.02±1.43b	6.13±0.23a	113.54±2.80b	53.98±0.64a
A2B2C2	33.28±0.26b	6.17±0.18a	116.49±1.83b	52.91±0.67a
$η A 2$	0.637	0.250	0.721	0.096
$η B 2$	0.911	0.742	0.913	0.041
$η C 2$	0.678	0.299	0.851	0.305
$η A × B × C 2$	0.147	0.029	0.113	0.001

Tab.7 Effects of the diameter of seedling tray,microbial agent and transplanting leaf age on K fertilizer efficiency of flue-cured tobacco

处理 Treatments	钾肥干物质积累效率/(kg/kg) K-FDAE	钾肥烟叶利用效率/(kg/kg) K-FLAE	钾肥吸收效率/(g/kg) K-FAE	钾素烟叶生产效率/(kg/kg) K-LPE
A1	35.48±1.97a	6.51±0.58a	124.44±8.31a	52.31±2.05a
A2	33.94±1.65b	6.31±0.22a	119.14±7.15b	53.02±2.24a
B1	36.58±2.02a	6.71±0.37a	127.11±7.88a	52.89±2.60a
B2	32.84±0.92b	6.11±0.25a	116.47±3.07b	52.44±1.62a
C1	33.86±2.02b	6.29±0.42a	117.85±5.28b	53.39±2.08a
C2	35.56±2.68a	6.53±0.45a	125.74±8.53a	51.94±2.00a
A1B1C1	36.38±0.29ab	6.81±0.11a	125.72±0.19ab	54.16±0.93a
A1B1C2	39.48±1.48a	7.12±0.56a	136.19±2.36a	52.20±3.21a
A1B2C1	32.36±0.35b	5.81±0.11a	115.62±2.76b	50.24±0.28a
A1B2C2	33.72±0.03b	6.33±0.20a	120.25±0.12ab	52.64±1.58a
A2B1C1	34.70±0.96ab	6.44±0.22a	116.53±2.34b	55.21±0.78a
A2B1C2	35.76±0.15ab	6.50±0.08a	130.03±3.67ab	50.01±2.04a
A2B2C1	32.02±1.43b	6.13±0.23a	113.54±2.80b	53.98±0.64a
A2B2C2	33.28±0.26b	6.17±0.18a	116.49±1.83b	52.91±0.67a
$η A 2$	0.637	0.250	0.721	0.096
$η B 2$	0.911	0.742	0.913	0.041
$η C 2$	0.678	0.299	0.851	0.305
$η A × B × C 2$	0.147	0.029	0.113	0.001

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