Effects of Fertilizer Reduction Combined with Organic Fertilizer on Physicochemical Properties and Microbial Community Structure of Tobacco-planting Soil

doi:10.7668/hbnxb.20194778

Abstract

Abstract:

To explore the effects of fertilizer reduction and organic fertilizer application on physicochemical properties and microbial community structure of tobacco-planting soil,and provide theoretical reference for fertilizer reduction and rational application of organic fertilizer in tobacco production.Using conventional fertilization without organic fertilizer as control(CK),Illumina high-throughput sequencing technology combined with bioinformatics,the changes of soil physical and chemical properties and microbial community structure under different treatments such as 10% reduction of fertilizer(T1),10% reduction of fertilizer+sesame cake fertilizer(T2),10% reduction of fertilizer + humic acid fertilizer(T3),and 10% reduction of fertilizer + sesame cake fertilizer + humic acid fertilizer(T4)were analyzed.The results showed that compared with CK,soil nutrient content and soil enzyme activity decreased under T1 treatment,and soil physical properties were slightly improved.Combined with organic fertilizer,soil nutrient and physical properties were further improved,bulk density decreased,moisture content and porosity increased,and the contents of available phosphorus and available potassium under T2 and T4 treatment were significantly higher than those under CK and T1 treatment.The enzyme activity of soil treated with organic fertilizer increased significantly.Combined application of organic fertilizer increased the bacteria and fungi in tobacco-growing soil,among which the dominant bacteria were Proteobacteria and Actinobacteriota.Followed by Firmicutes,Bacteroidota,Chloroflexi and Acidobacteriota.The dominant fungal groups are Ascomycota,Anthophyta,Mortierellomycota,Chlorophyta,Ciliophora and Basidiomycota.The Alpha diversity index showed that the reduction of fertilizer decreased the richness of microbial community,but the combined application of organic fertilizer increased the diversity index of bacterial and fungal communities,and the bacterial community richness increased more significantly.RDA analysis showed that the important soil physicochemical factors affecting soil microbial community structure and diversity included organic matter,available potassium,alkali-hydrolyzed nitrogen,available phosphorus and soil physical properties,etc.Soil physicochemical factors had a greater impact on bacterial community structure.In conclusion,under the condition of fertilizer reduction,organic fertilizer combined with organic fertilizer can significantly improve soil nutrients,physical properties,soil enzyme activity and microflora structure,especially when combined with sesame cake fertilizer and humic acid fertilizer treatment.

Key words: Tabacco, Fertilizer reduction, Organic fertilizer, Tobacco-growing soil, Physicochemical properties, Microbial community structure

SUN Zijun, ZHONG Guoxing, ZHANG Shaobo, YANG Xin, PENG Jinliang, GUO Wei, LIANG Yaoxing, LI Huaiyuan, BAI Yunfeng, CHEN Jianjun, DENG Shiyuan. Effects of Fertilizer Reduction Combined with Organic Fertilizer on Physicochemical Properties and Microbial Community Structure of Tobacco-planting Soil[J]. Acta Agriculturae Boreali-Sinica, 2024, 39(3): 146-158. doi: 10.7668/hbnxb.20194778.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.20194778

http://www.hbnxb.net/EN/Y2024/V39/I3/146

Figures/Tables 18

References 55

[1]	Nannipieri P, Ascher J, Ceccherini M T, Landi L, Pietramellara G, Renella G. Microbial diversity and soil functions[J]. European Journal of Soil Science, 2003, 54(4):655-670.doi:10.1046/j.1351-0754.2003.0556.x.
[2]	Jiang R, Wang M E, Chen W P, Li X Z, Balseiro-Romero M. Changes in the integrated functional stability of microbial community under chemical stresses and the impacting factors in field soils[J]. Ecological Indicators, 2020, 110:105919.doi:10.1016/j.ecolind.2019.105919.
[3]	Bender S F, Schulz S, Martínez-Cuesta R, Laughlin R J, Kublik S, Pfeiffer-Zakharova K, Vestergaard G, Hartman K, Parladé E, Römbke J, Watson C J, Schloter M, van der Heijden M G A. Simplification of soil biota communities impairs nutrient recycling and enhances above-and belowground nitrogen losses[J]. The New Phytologist, 2023, 240(5):2020-2034.doi:10.1111/nph.19252.
[4]	徐永刚, 宇万太, 马强, 周桦. 长期不同施肥制度对潮棕壤微生物生物量碳、氮及细菌群落结构的影响[J]. 应用生态学报, 2010, 21(8):2078-2085.doi:10.13287/j.1001-9332.2010.0288.
	Xu Y G, Yu W T, Ma Q, Zhou H. Effects of long-term fertilizations on microbial biomass C and N and bacterial community structure in an aquic brown soil[J]. Chinese Journal of Applied Ecology, 2010, 21(8):2078-2085.
[5]	Han J P, Shi J C, Zeng L Z, Xu J M, Wu L S. Effects of nitrogen fertilization on the acidity and salinity of greenhouse soils[J]. Environmental Science and Pollution Research International, 2015, 22(4):2976-2986.doi:10.1007/s11356-014-3542-z. pmid: 25226832
[6]	尹鹏达, 朱文旭, 赵丽娜, 许楠, 张会慧, 焦玉生, 赵光伟, 孙广玉. 氮磷钾配施对填充型烤烟烟碱含量的影响[J]. 应用生态学报, 2011, 22(5):1189-1194.doi:10.13287/j.1001-9332.2011.0162.
	Yin P D, Zhu W X, Zhao L N, Xu N, Zhang H H, Jiao Y S, Zhao G W, Sun G Y. Effects of combined fertilization of N,P,and K on nicotine content of filling type flue-cured tobacco[J]. Chinese Journal of Applied Ecology, 2011, 22(5):1189-1194.
[7]	柳玲玲, 芶久兰, 何佳芳, 范成五, 秦松. 生物有机肥对连作马铃薯及土壤生化性状的影响[J]. 土壤, 2017, 49(4):706-711.doi:10.13758/j.cnki.tr.2017.04.010.
	Liu L L, Gou J L, He J F, Fan C W, Qin S. Effects of different bio-organic fertilizers on potato production and soil biochemical characteristics in a continuous cropping system[J]. Soils, 2017, 49(4):706-711.
[8]	林小兵, 柳开楼, 黄尚书, 何绍浪, 徐小林, 周琦娜, 钟义军. 长期施肥对旱地红壤微生物生物量碳、氮和酶活性的影响[J]. 华北农学报. 2023, 38(4):119-128.doi:10.7668/hbnxb.20193875.
	Lin X B, Liu K L, Huang S S, He S L, Xu X L, Zhou Q N, Zhong Y J. Effects of long-term fertilization on microbial biomass carbon,nitrogen and enzyme activities in upland red soil[J]. Acta Agriculturae Boreali-Sinica, 2023, 38(4):119-128. doi: 10.7668/hbnxb.20193875
[9]	王娟娟, 朱紫娟, 钱晓晴, 王桂良. 减施化肥与不同有机肥配施对稻季土壤细菌群落结构的影响[J]. 土壤, 2021, 53(5):983-990.doi:10.13758/j.cnki.tr.2021.05.013.
	Wang J J, Zhu Z J, Qian X Q, Wang G L. Effects of reducing chemical fertilizer combined with application of different organic fertilizers on soil bacterial community structure during rice season[J]. Soils, 2021, 53(5):983-990.
[10]	黄国勤, 王兴祥, 钱海燕, 张桃林, 赵其国. 施用化肥对农业生态环境的负面影响及对策[J]. 生态环境, 2004(4):656-660.doi:10.16258/j.cnki.1674-5906.2004.04.055.
	Huang G Q, Wang X X, Qian H Y, Zhang T L, Zhao Q G. Negative impact of inorganic fertilizes application on agricultural environment and its countermeasures[J]. Ecology and Environmental Sciences, 2004(4):656-660.
[11]	杨小东. 长期施肥对三种土壤类型酶活性及硝化微生物丰度的影响[D]. 北京: 中国农业科学院, 2020.doi:10.27630/d.cnki.gznky.2020000663..
	Yang X D. Effects of long-term fertilization on enzyme activities and nitrogenous microbial abundance in three soil types[D]. Beijing: Chinese Academy of Agricultural Sciences, 2020.
[12]	彭先容, 王帅, 曾卓华, 王佳乐, 王妍, 冯蓉, 秦梦婵, 赵敬坤, 卢明, 张跃强. 有机肥替代化肥比例对红心柚产量和品质及土壤养分的影响[J]. 中国土壤与肥料, 2023(11):110-119.doi:10.11838/sfsc.1673-6257.22647.
	Peng X R, Wang S, Zeng Z H, Wang J L, Wang Y, Feng R, Qin M C, Zhao J K, Lu M, Zhang Y Q. Effects of partial substitution of chemical fertilizer with organic fertilizer on the yield and fruits qualities of red pomelo and soil properties[J]. Soil and Fertilizer Sciences in China, 2023(11):110-119.
[13]	邵慧芸, 李紫玥, 刘丹, 李熠凡, 鲁璐, 王旭东, 张阿凤, 王彦丽. 有机肥施用量对土壤有机碳组分和团聚体稳定性的影响[J]. 环境科学, 2019, 40(10):4691-4699.doi:10.13227/j.hjkx.201902013.
	Shao H Y, Li Z Y, Liu D, Li Y F, Lu L, Wang X D, Zhang A F, Wang Y L. Effects of manure application rates on the soil carbon fractions and aggregate stability[J]. Environmental Science, 2019, 40(10):4691-4699.
[14]	任改弟, 张苗, 张文越, 郭德杰, 马艳. 不同来源有机物料对菜用蚕豆生长和品质及根际土壤性状的影响[J]. 土壤, 2022, 54(4):740-749.doi:10.13758/j.cnki.tr.2022.04.011.
	Ren G D, Zhang M, Zhang W Y, Guo D J, Ma Y. Effects of organic materials from different sources on growth and quality of vegetable broad beans and properties of rhizosphere soil[J]. Soils, 2022, 54(4):740-749.
[15]	王树会, 耿素祥. 过量施肥对烤烟生长发育和产质的影响[J]. 中国农业科技导报, 2010, 12(5):116-122.doi:10.3969/j.issn.1008-0864.2010.05.20.
	Wang S H, Geng S X. Effect of excessive fertilization on growth,development,yield and quality of flue-cured tobacco[J]. Journal of Agricultural Science and Technology, 2010, 12(5):116-122.
[16]	王艳群, 彭正萍, 薛世川, 杨云马, 周亚鹏, 赵立宾. 过量施肥对设施农田土壤生态环境的影响[J]. 农业环境科学学报, 2005, 24(S1):81-84.doi:10.3321/j.issn:1672-2043.2005.z1.020.
	Wang Y Q, Peng Z P, Xue S C, Yang Y M, Zhou Y P, Zhao L B. Effects of excessive fertilization on soil ecological environment of protected farmland[J]. Journal of Agro-Environment Science, 2005, 24(S1):81-84.
[17]	孔德宁, 康国栋, 李鹏, 沈学善, 刘满强, 李辉信, 胡锋, 焦加国. 化肥减施条件下配施有机肥对旱地紫色土有机碳活性组分的影响[J]. 生态学杂志, 2021, 40(4):1073-1080.doi:10.13292/j.1000-4890.202104.002.
	Kong D N, Kang G D, Li P, Shen X S, Liu M Q, Li H X, Hu F, Jiao J G. Effects of combined application of organic fertilizer on the active components of organic carbon in upland purple soil under reducing chemical fertilizer application[J]. Chinese Journal of Ecology, 2021, 40(4):1073-1080. doi: 10.13292/j.1000-4890.202104.002
[18]	张启明, 陈仁霄, 管成伟, 苑举民, 何宽信. 不同有机物料对土壤改良和烤烟产质量的影响[J]. 土壤, 2018, 50(5):929-933.doi:10.13758/j.cnki.tr.2018.05.011.
	Zhang Q M, Chen R X, Guan C W, Yuan J M, He K X. Effects of different organic materials on soil improvement and tobacco yield and quality[J]. Soils, 2018, 50(5):929-933.
[19]	杨德廉, 周昕, 李更新, 杜传印, 管恩森, 马夙静, 张彭, 刘振宇, 李祥英. 有机肥施用对烟田土壤酶活性的影响[J]. 中国农学通报, 2020, 36(15):60-67.doi:10.11924/j.issn.1000-6850.casb19010118.
	Yang D L, Zhou X, Li G X, Du C Y, Guan E S, Ma S J, Zhang P, Liu Z Y, Li X Y. Organic fertilizers application improves soil enzyme activity in tobacco field[J]. Chinese Agricultural Science Bulletin, 2020, 36(15):60-67. doi: 10.11924/j.issn.1000-6850.casb19010118
[20]	张云伟, 徐智, 汤利, 李艳红, 宋建群, 徐健钦. 不同有机肥对烤烟根际土壤微生物的影响[J]. 应用生态学报, 2013, 24(9):2551-2556.doi:10.13287/j.1001-9332.2013.0504.
	Zhang Y W, Xu Z, Tang L, Li Y H, Song J Q, Xu J Q. Effects of different organic fertilizers on the microbes in rhizospheric soil of flue-cured tobacco[J]. Chinese Journal of Applied Ecology, 2013, 24(9):2551-2556.
[21]	蔡秋华, 赵正雄, 左进香, 李宜成, 黄俊杰. 有机肥配施减量化肥对烤烟青枯病及其根际微生物的影响[J]. 烟草科技, 2018, 51(11):20-27.doi:10.16135/j.issn1002-0861.2018.0045.
	Cai Q H, Zhao Z X, Zuo J X, Li Y C, Huang J J. Effects of organic fertilizers combined with reduced chemical fertilizers on tobacco bacterial wilt disease and rhizosphere microorganism[J]. Tobacco Science & Technology, 2018, 51(11):20-27.
[22]	杜森, 高祥照, 全国农业技术推广服务中心. 土壤分析技术规范[M]. 2版. 北京: 中国农业出版社, 2006:256.
	Du S, Gao X Z, National Agricultural Technology Extension Service Center. Technical specification for soil analysis[M]. 2nd edition. Beijing: China Agriculture Press, 2006:256.
[23]	关松荫. 土壤酶及其研究法[M]. 北京: 农业出版社, 1986:376.
	Guan S Y. Soil enzymes and their research methods[M]. Beijing: Agricultural Press, 1986:376.
[24]	张北赢, 陈天林, 王兵. 长期施用化肥对土壤质量的影响[J]. 中国农学通报, 2010, 26(11):182-187.doi:10.11924/j.issn.1000-6850.2009-2796.
	Zhang B Y, Chen T L, Wang B. Effects of long-term uses of chemical fertilizers on soil quality[J]. Chinese Agricultural Science Bulletin, 2010, 26(11):182-187. doi: 10.11924/j.issn.1000-6850.2009-2796
[25]	万连杰, 何满, 田洋, 郑永强, 吕强, 谢让金, 马岩岩, 邓烈, 易时来, 李建. 有机肥替代化肥比例对椪柑生长发育、产量和土壤生物学特性的影响[J]. 植物营养与肥料学报. 2022, 28(4):675-687.doi:10.11674/zwyf.2021446.
	Wan L J, He M, Tian Y, Zheng Y Q, Lü Q, Xie R J, Ma Y Y, Deng L, Yi S L, Li J. Effects of partial substitution of chemical fertilizer with organic fertilizer on Ponkan growth and yield and soil biological properties[J]. Journal of Plant Nutrition and Fertilizers, 2022, 28(4):675-687.
[26]	马俊永, 陈金瑞, 李科江, 曹彩云, 郑春莲. 施用化肥和秸秆对土壤有机质含量及性质的影响[J]. 河北农业科学, 2006, 10(4):44-47.doi:10.16318/j.cnki.hbnykx.2006.04.012.
	Ma J Y, Chen J R, Li K J, Cao C Y, Zheng C L. The effect of chemical and organic straw fertilization on the content and properties of soil organic matter[J]. Journal of Hebei Agricultural Sciences, 2006, 10(4):44-47.
[27]	Yang Z C, Zhao N, Huang F, Lü Y Z. Long-term effects of different organic and inorganic fertilizer treatments on soil organic carbon sequestration and crop yields on the North China Plain[J]. Soil and Tillage Research, 2015, 146:47-52.doi:10.1016/j.still.2014.06.011.
[28]	Zhao H Y, Lakshmanan P, Wang X Z, Xiong H Y, Yang L S, Liu B, Shi X J, Chen X P, Wang J, Zhang Y Q, Zhang F S. Global reactive nitrogen loss in orchard systems:a review[J]. The Science of the Total Environment, 2022, 821:153462.doi:10.1016/j.scitotenv.2022.153462.
[29]	肖让, 张永玲, 赵芸晨, 郭世乾, 崔增团, 师伟杰. 化肥减量配施有机肥对日光温室土壤质量及茄子产量、品质的影响[J]. 华北农学报, 2023, 38(2):188-198.doi:10.7668/hbnxb.20193466.
	Xiao R, Zhang Y L, Zhao Y C, Guo S Q, Cui Z T, Shi W J. Effects of chemical fertilizer reduction and organic fertilizer application on soil quality,eggplant yield and quality in solar greenhouse[J]. Acta Agriculturae Boreali-Sinica, 2023, 38(2):188-198. doi: 10.7668/hbnxb.20193466
[30]	吴宪. 化肥减量配施有机肥和秸秆对小麦——玉米田土壤微生物和线虫群落的影响[D]. 北京: 中国农业科学院, 2021.doi:10.27630/d.cnki.gznky.2021.000626.
	Wu X. Effects of chemical fertilizer reduction combined with organic fertilizer and straw on soil microorganism and nematode community in wheat-corn field[D]. Beijing: Chinese Academy of Agricultural Sciences, 2021.
[31]	温延臣, 张曰东, 袁亮, 李伟, 李燕青, 林治安, 赵秉强. 商品有机肥替代化肥对作物产量和土壤肥力的影响[J]. 中国农业科学, 2018, 51(11):2136-2142.doi:10.3864/j.issn.0578-1752.2018.11.011.
	Wen Y C, Zhang Y D, Yuan L, Li W, Li Y Q, Lin Z A, Zhao B Q. Crop yield and soil fertility response to commercial organic fertilizer substituting chemical fertilizer[J]. Scientia Agricultura Sinica, 2018, 51(11):2136-2142. doi: 10.3864/j.issn.0578-1752.2018.11.011
[32]	蒲全明, 杨鹏, 邓榆川, 向承勇, 林邦民, 刘莉莎, 施松梅, 何泽民, 雍磊. 不同施肥方式对冬春茬甘蓝根际土壤酶活性、土壤养分及品质的影响[J]. 中国农业科技导报, 2020, 22(7):130-139.doi:10.13304/j.nykjdb.2019.0525.
	Pu Q M, Yang P, Deng Y C, Xiang C Y, Lin B M, Liu L S, Shi S M, He Z M, Yong L. Effects of different fertilization methods on soil enzyme activity,soil nutrients and quality of spring cabbage[J]. Journal of Agricultural Science and Technology, 2020, 22(7):130-139.
[33]	唐继伟, 徐久凯, 温延臣, 田昌玉, 林治安, 赵秉强. 长期单施有机肥和化肥对土壤养分和小麦产量的影响[J]. 植物营养与肥料学报, 2019, 25(11):1827-1834.doi:10.11674/zwyf.18436.
	Tang J W, Xu J K, Wen Y C, Tian C Y, Lin Z A, Zhao B Q. Effects of organic fertilizer and inorganic fertilizer on the wheat yields and soil nutrients under long-term fertilization[J]. Journal of Plant Nutrition and Fertilizers, 2019, 25(11):1827-1834.
[34]	仁增草, 武均, 彭敏, 宋雪峰, 王月胜, 蔡立群. 不同施肥处理对芹菜农田土壤理化性状及产量的影响[J]. 中国农学通报, 2022, 38(16):86-90.doi:10.11924/j.issn.1000-6850.casb2021-0587.
	Ren Z C, Wu J, Peng M, Song X F, Wang Y S, Cai L Q. Effects of different fertilization treatments on soil physical and chemical properties and yield of celery[J]. Chinese Agricultural Science Bulletin, 2022, 38(16):86-90. doi: 10.11924/j.issn.1000-6850.casb2021-0587
[35]	邓帅帅, 闫浩芳, 张川, 王国庆, 张建云, 梁少威, 蒋建辉. 灌溉量与减氮配施有机肥模式对温室黄瓜及土壤的影响[J]. 农业工程学报, 2023, 39(19):93-102.doi:10.11975/j.issn.1002-6819.202305150.
	Deng S S, Yan H F, Zhang C, Wang G Q, Zhang J Y, Liang S W, Jiang J H. Effects of irrigation amounts and nitrogen reduction combined with organic fertilizer pattern on greenhouse cucumber and soil[J]. Transactions of the Chinese Society of Agricultural Engineering, 2023, 39(19):93-102.
[36]	Amadou A, Song A L, Tang Z X, Li Y L, Wang E Z, Lu Y Q, Liu X D, Yi K K, Zhang B, Fan F L. The effects of organic and mineral fertilization on soil enzyme activities and bacterial community in the below-and above-ground parts of wheat[J]. Agronomy, 2020, 10(10):1452.doi:10.3390/agronomy10101452.
[37]	Li F, Zang S Y, Liu Y N, Li L, Ni H W. Effect of freezing thawing cycle on soil active organic carbon fractions and enzyme activities in the wetland of Sanjiang Plain,Northeast China[J]. Wetlands, 2020, 40(1):167-177.doi:10.1007/s13157-019-01164-9.
[38]	Tian S Y, Zhu B J, Yin R, Wang M W, Jiang Y J, Zhang C Z, Li D M, Chen X Y, Kardol P, Liu M Q. Organic fertilization promotes crop productivity through changes in soil aggregation[J]. Soil Biology and Biochemistry, 2022, 165:108533.doi:10.1016/j.soilbio.2021.108533.
[39]	Cleveland C C, Liptzin D. C:N:P stoichiometry in soil:is there a Redfield ratio for the microbial biomass?[J]. Biogeochemistry, 2007, 85(3):235-252.doi:10.1007/s10533-007-9132-0.
[40]	田善义, 王明伟, 成艳红, 陈小云, 李大明, 胡锋, 刘满强. 化肥和有机肥长期施用对红壤酶活性的影响[J]. 生态学报, 2017, 37(15):4963-4972.doi:10.5846/stxb201604270800.
	Tian S Y, Wang M W, Cheng Y H, Chen X Y, Li D M, Hu F, Liu M Q. Long-term effects of chemical and organic amendments on red soil enzyme activities[J]. Acta Ecologica Sinica, 2017, 37(15):4963-4972.
[41]	张璐, 阎海涛, 任天宝, 李帅, 杨永锋, 彭桂新, 于建春, 刘国顺. 有机物料对植烟土壤养分、酶活性和微生物群落功能多样性的影响[J]. 中国烟草学报, 2019, 25(2):55-62.doi:10.16472/j.chinatobacco.2018.195.
	Zhang L, Yan H T, Ren T B, Li S, Yang Y F, Peng G X, Yu J C, Liu G S. Effects of organic matter on nutrient,enzyme activity and functional diversity of microbial community in tobacco planting soil[J]. Acta Tabacaria Sinica, 2019, 25(2):55-62.
[42]	张延慧, 刘宇, 韩莹, 董馨宇, 郭探文, 闫秋艳, 闫双堆. 不同比例硫酸铵替代尿素对玉米根际土壤环境及微生物群落的影响[J]. 环境科学, 2023:1-17.doi:10.13227/j.hjkx.202307065.
	Zhang Y H, Liu Y, Han Y, Dong X Y, Guo T W, Yan Q Y, Yan S D. Effects of different proportions of ammonium sulfate replacing urea on soil nutrients and rhizosphere microbial communities[J]. Environmental Science, 2023:1-17.
[43]	白玲. 有机无机肥配施对滴灌棉田氮素转化生物学过程的影响研究[D]. 石河子: 石河子大学, 2014.doi:10.7666/d.D607533.
	Bai L. Effect of combined application of organic and inorganic fertilizers on biological process of nitrogen transformation in drip irrigation cotton field[D]. Shihezi: Shihezi University, 2014.
[44]	于会泳, 宋晓丽, 王树声, 曹丽君, 郭利, 王晓丽, 彭功银. 低分子量有机酸对植烟土壤酶活性和细菌群落结构的影响[J]. 中国农业科学, 2015, 48(24):4936-4947.doi:10.3864/j.issn.0578-1752.2015.24.008.
	Yu H Y, Song X L, Wang S S, Cao L J, Guo L, Wang X L, Peng G Y. Effects of low molecular weight organic acids on soil enzymes activities and bacterial community structure[J]. Scientia Agricultura Sinica, 2015, 48(24):4936-4947.
[45]	焦晓丹, 吴凤芝. 土壤微生物多样性研究方法的进展[J]. 土壤通报, 2004, 35(6):789-792.doi:10.3321/j.issn:0564-3945.2004.06.026.
	Jiao X D, Wu F Z. Progress of the methods for studying soil microbial diversity[J]. Chinese Journal of Soil Science, 2004, 35(6):789-792.
[46]	王超, 刘明庆, 黄思杰, 李妍, 田伟, 陈秋会, 王磊, 席运官. 不同施肥处理对有机种植土壤微生物区系的影响[J]. 江苏农业科学, 2019, 47(20):266-272.doi:10.15889/j.issn.1002-1302.2019.20.061.
	Wang C, Liu M Q, Huang S J, Li Y, Tian W, Chen Q H, Wang L, Xi Y G. Effects of different fertilization treatments on microflora of organic planting soils[J]. Jiangsu Agricultural Sciences, 2019, 47(20):266-272.
[47]	黄伟, 张俊花, 刘倩男, 张珍珍, 王卫忠, 李凤荣, 李刚. 微生物菌肥对生菜土壤酶活性和微生物数量的影响[J]. 湖北农业科学, 2019, 58(22):54-57,64.doi:10.14088/j.cnki.issn0439-8114.2019.22.013.
	Huang W, Zhang J H, Liu Q N, Zhang Z Z, Wang W Z, Li F R, Li G. Effects of the microbial fertilizers on enzymic activity and microorganism in lettuce soil[J]. Hubei Agricultural Sciences, 2019, 58(22):54-57,64.
[48]	Gougoulias C, Clark J M, Shaw L J. The role of soil microbes in the global carbon cycle:tracking the below-ground microbial processing of plant-derived carbon for manipulating carbon dynamics in agricultural systems[J]. Journal of the Science of Food and Agriculture, 2014, 94(12):2362-2371.doi:10.1002/jsfa.6577. pmid: 24425529
[49]	薛超群, 奚家勤, 王建伟, 杨立均. 腐植酸用量对土壤微生物数量和烟叶香气品质的影响[J]. 腐植酸, 2016(6): 44.doi:10.19451/j.cnki.issn1671-9212.2016.06.013.
	Xue C Q, Xi J Q, Wang J W, Yang L J. Effects of humic acid dosage on the quantity of soil microbes and the aroma quality of tobacco leaves[J]. Humic Acid, 2016(6):44.
[50]	温烜琳, 马宜林, 周俊学, 高佳凯, 张水源, 张枫, 申欣, 王悦华, 申洪涛, 元野, 李友军, 刘领. 腐熟羊粪有机肥配施无机肥对植烟土壤微生物群落结构和多样性的影响[J]. 土壤, 2023, 55(4):804-811.doi:10.13758/j.cnki.tr.2023.04.014.
	Wen X L, Ma Y L, Zhou J X, Gao J K, Zhang S Y, Zhang F, Shen X, Wang Y H, Shen H T, Yuan Y, Li Y J, Liu L. Effects of combined application of organic manure and inorganic fertilizer on structure and diversity of microbial community in tobacco-growing soil[J]. Soils, 2023, 55(4):804-811.
[51]	宋兆齐, 王莉, 刘秀花, 梁峰. 云南4处酸性热泉中的变形菌门细菌多样性[J]. 河南农业大学学报, 2016, 50(3):376-382.doi:10.16445/j.cnki.1000-2340.2016.03.015.
	Song Z Q, Wang L, Liu X H, Liang F. The diversities of Proteobacteria in four acidic hot springs in Yunnan[J]. Journal of Henan Agricultural University, 2016, 50(3):376-382.
[52]	荣勤雷. 有机肥/秸秆替代化肥模式对设施菜田土壤团聚体微生物特性的影响[D]. 北京: 中国农业科学院, 2018.
	Rong Q L. Effects of partial substitution of chemical fertilizer with organic amendments on microbial characteristics of soil aggregates in greenhouse vegetable production[D]. Beijing: Chinese Academy of Agricultural Sciences, 2018.
[53]	Souza R C, Mendes I C, Reis-Junior F B, Carvalho F M, Nogueira M A, Vasconcelos A T R, Vicente V A, Hungria M. Shifts in taxonomic and functional microbial diversity with agriculture:how fragile is the Brazilian Cerrado?[J]. BMC Microbiology, 2016, 16:42.doi:10.1186/s12866-016-0657-z.
[54]	任春光, 苏文文, 潘丽珊, 韩振诚, 吴迪, 李良良, 王加国, 李苇洁. 基于高通量测序研究猕猴桃苗不同生育期根际真菌群落结构及多样性[J]. 土壤, 2021, 53(3):545-554.doi:10.13758/j.cnki.tr.2021.03.014.
	Ren C G, Su W W, Pan L S, Han Z C, Wu D, Li L L, Wang J G, Li W J. Fungal community structures and diversities in rhizosphere soils in different growth stages of kiwifruit seedlings based on high-throughput sequencing[J]. Soils, 2021, 53(3):545-554.
[55]	Zhang X H, Song Y L, Yang X, Hu C, Wang K Q. Regulation of soil enzyme activity and bacterial communities by food waste compost application during field tobacco cultivation cycle[J]. Applied Soil Ecology, 2023, 192:105016.doi:10.1016/j.apsoil.2023.105016.

处理 Treatment	N	P₂O₅	K₂O	芝麻饼肥 Sesame cake	腐殖酸肥 Humus
CK	147.0	142.0	483.0	0	0
T1	132.3	127.8	434.7	0	0
T2	132.3	127.8	434.7	600	0
T3	132.3	127.8	434.7	0	450
T4	132.3	127.8	434.7	600	450

处理 Treatment	N	P₂O₅	K₂O	芝麻饼肥 Sesame cake	腐殖酸肥 Humus
CK	147.0	142.0	483.0	0	0
T1	132.3	127.8	434.7	0	0
T2	132.3	127.8	434.7	600	0
T3	132.3	127.8	434.7	0	450
T4	132.3	127.8	434.7	600	450

处理 Treatment	有机质/(g/kg) Organic matter	碱解氮/(mg/kg) Available nitrogen	有效磷/(mg/kg) Available phosphorus	速效钾/(mg/kg) Available potassium	容重/(g/cm³) Soil bulk density	含水率/% Soil moisture content	孔隙度/% Soil porosity
CK	36.80±0.42a	23.93±3.00b	62.62±2.22c	517.89±4.37c	1.62±0.01a	31.02±0.01d	38.46±0.51d
T1	35.88±0.10a	16.65±2.31d	47.57±3.59d	347.13±3.20d	1.54±0.02a	34.64±0.00c	41.52±0.81c
T2	37.97±0.39a	26.00±3.76b	89.68±1.32a	566.41±6.37b	1.27±0.03d	40.47±0.01a	51.69±0.98a
T3	37.09±0.26a	21.12±1.50c	51.37±0.98d	652.40±6.48a	1.38±0.02c	37.59±0.00b	47.59±0.58b
T4	38.09±0.37a	35.24±3.42a	80.66±2.90b	642.80±14.36a	1.44±0.00b	36.43±0.00bc	45.14±0.11b

处理 Treatment	有机质/(g/kg) Organic matter	碱解氮/(mg/kg) Available nitrogen	有效磷/(mg/kg) Available phosphorus	速效钾/(mg/kg) Available potassium	容重/(g/cm³) Soil bulk density	含水率/% Soil moisture content	孔隙度/% Soil porosity
CK	36.80±0.42a	23.93±3.00b	62.62±2.22c	517.89±4.37c	1.62±0.01a	31.02±0.01d	38.46±0.51d
T1	35.88±0.10a	16.65±2.31d	47.57±3.59d	347.13±3.20d	1.54±0.02a	34.64±0.00c	41.52±0.81c
T2	37.97±0.39a	26.00±3.76b	89.68±1.32a	566.41±6.37b	1.27±0.03d	40.47±0.01a	51.69±0.98a
T3	37.09±0.26a	21.12±1.50c	51.37±0.98d	652.40±6.48a	1.38±0.02c	37.59±0.00b	47.59±0.58b
T4	38.09±0.37a	35.24±3.42a	80.66±2.90b	642.80±14.36a	1.44±0.00b	36.43±0.00bc	45.14±0.11b

处理 Treatment	蛋白酶 Protease	酸性磷酸酶 Acid phosphatase	蔗糖酶 Sucrase	脲酶 Urease
CK	11.63±0.14b	11.77±0.08c	3.95±0.04a	5.53±0.04c
T1	10.90±0.25c	8.03±0.18d	3.93±0.22a	4.36±0.16d
T2	13.96±0.19a	15.55±0.09a	4.12±0.02a	7.53±0.07a
T3	13.69±0.03a	12.70±0.25b	4.06±0.03a	5.97±0.04b
T4	11.95±0.13b	13.13±0.16b	4.26±0.06a	7.43±0.06a

Please choose a citation manager

Content to export