The Prediction of Soil Fungal Community Structure and Function Based on Different Organic Fertilizer Application Rates

doi:10.7668/hbnxb.20194191

Abstract

Abstract:

In order to provide a theoretical basis for rational application of organic fertilizers and ensure the healthy development of soil ecosystems in corn fields,the effects of soil fungal communities under different levels of organic fertilizer application for the composition,diversity,and functional characteristic were studied.In the meantime,a field experiment was used,with a total of 4 treatments were set up,and the high-throughput sequencing and FUNGuild method were used to analyze the soil fungal diversity,community structure and functional groups in different fertilization rates.The results showed that the increased application of organic fertilizer could increase the content of soil organic matter,available phosphorus,available potassium,urease,catalase,etc.,and it was positively correlated with the application amount of organic fertilizer.Applied organic fertilizers could increase the diversity of soil fungal communities and reduce their richness.The dominant fungal communities in the soil under different fertilization rates were Ascomycota,Mortierellomycota,Basidiomycota,and Olpidiomycota,the relative abundance of Ascomycota and Basidiomycota was significantly higher in the treatment with a medium amount of cattle manure than in the control without organic fertilizer;from the perspective of fungal genera,the dominant genera include Fusarium,Humicola,Olpidium,and Microidium.The application of organic fertilizer increased the relative abundance of symbiotic and saprophytic nutrients,and with the increase of organic fertilizer application,the abundance of pathological nutrients showed a decreasing trend;the number of woody saprophytic fungi in the treatment with increased organic fertilizer application was significantly higher than that in the treatment without organic fertilizer application,while the number of plant and animal pathogens was lower than the control.Therefore,it is believed that increasing a certain amount of organic fertilizer application could optimize the soil microbial environment and promote the increase of corn plant yield.

Key words: Fungi, Organic fertilizer, Diversity, Community structure, High throughput sequencing

MA Jianhua, YANG Bo, LIU Chang, WANG Yan, MA Kun. The Prediction of Soil Fungal Community Structure and Function Based on Different Organic Fertilizer Application Rates[J]. Acta Agriculturae Boreali-Sinica, 2023, 38(6): 118-126. doi: 10.7668/hbnxb.20194191.

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Figures/Tables 8

References 34

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处理 Treatment	全氮/ (g/kg) Total nitrogen	全磷/ (g/kg) Total phosphorus	有机质/ (g/kg) Organic matter	碱解氮/ (mg/kg) Alkali hydrolyzed nitrogen	速效磷/ (g/kg) Olsen-P	速效钾/ (mg/kg) Available K	脲酶/ (mg/g) Urease	过氧化氢酶/ (mL/(g·h)) Catalase	碱性磷酸酶/ (mg/g) Alkaline phosphatase	pH
T0	0.22±0.07a	0.60±0.08a	4.12±0.89c	18.06±5.13a	10.17±3.71a	353.33±190.88ab	2.04±1.51a	0.25±0.03a	0.30±0.19a	8.24±0.04a
T1	0.40±0.08a	0.83±0.04a	4.72±1.55bc	23.31±7.87a	8.64±3.78a	253.33±76.38b	4.20±2.31a	0.25±0.05a	0.27±0.09a	8.25±0.01a
T2	0.67±0.52a	0.87±0.32a	6.00±2.28ab	28.77±8.20a	15.97±8.44a	330.00±45.83ab	4.36±1.37a	0.28±0.02a	0.57±0.29a	8.15±0.04b
T3	0.53±0.17a	0.84±0.05a	7.65±2.09a	26.46±11.20a	16.42±1.25a	520.00±216.33a	5.77±4.24a	0.28±0.02a	0.39±0.08a	8.20±0.04ab

处理 Treatment	全氮/ (g/kg) Total nitrogen	全磷/ (g/kg) Total phosphorus	有机质/ (g/kg) Organic matter	碱解氮/ (mg/kg) Alkali hydrolyzed nitrogen	速效磷/ (g/kg) Olsen-P	速效钾/ (mg/kg) Available K	脲酶/ (mg/g) Urease	过氧化氢酶/ (mL/(g·h)) Catalase	碱性磷酸酶/ (mg/g) Alkaline phosphatase	pH
T0	0.22±0.07a	0.60±0.08a	4.12±0.89c	18.06±5.13a	10.17±3.71a	353.33±190.88ab	2.04±1.51a	0.25±0.03a	0.30±0.19a	8.24±0.04a
T1	0.40±0.08a	0.83±0.04a	4.72±1.55bc	23.31±7.87a	8.64±3.78a	253.33±76.38b	4.20±2.31a	0.25±0.05a	0.27±0.09a	8.25±0.01a
T2	0.67±0.52a	0.87±0.32a	6.00±2.28ab	28.77±8.20a	15.97±8.44a	330.00±45.83ab	4.36±1.37a	0.28±0.02a	0.57±0.29a	8.15±0.04b
T3	0.53±0.17a	0.84±0.05a	7.65±2.09a	26.46±11.20a	16.42±1.25a	520.00±216.33a	5.77±4.24a	0.28±0.02a	0.39±0.08a	8.20±0.04ab

处理 Treatment	OTU个数 OTU number	Shannon指数 Shannon index	Simpson指数 Simpson index	Chao1指数 Chao1 index	ACE指数 ACE index	覆盖度 Coverage	谱系多样性 Phylogenetic diversity
T0	1 278.67±112.70a	5.41±0.11a	0.93±0.01a	1 675.88±343.92a	1 754.62±334.73a	0.99±0.00b	941.14±92.24a
T1	1 109.33±6.51ab	5.42±0.15a	0.93±0.01a	1 361.89±151.34ab	1 446.29±136.02ab	0.99±0.00ab	752.26±64.66ab
T2	924.67±186.15b	4.95±0.26b	0.91±0.02a	1 032.03±256.64b	1 082.69±266.75b	1.00±0.00ab	677.97±154.63b
T3	1 209.67±185.50a	5.45±0.20a	0.90±0.01a	1 560.40±282.32a	1 662.87±251.41a	0.99±0.00b	858.40±187.83ab

处理 Treatment	OTU个数 OTU number	Shannon指数 Shannon index	Simpson指数 Simpson index	Chao1指数 Chao1 index	ACE指数 ACE index	覆盖度 Coverage	谱系多样性 Phylogenetic diversity
T0	1 278.67±112.70a	5.41±0.11a	0.93±0.01a	1 675.88±343.92a	1 754.62±334.73a	0.99±0.00b	941.14±92.24a
T1	1 109.33±6.51ab	5.42±0.15a	0.93±0.01a	1 361.89±151.34ab	1 446.29±136.02ab	0.99±0.00ab	752.26±64.66ab
T2	924.67±186.15b	4.95±0.26b	0.91±0.02a	1 032.03±256.64b	1 082.69±266.75b	1.00±0.00ab	677.97±154.63b
T3	1 209.67±185.50a	5.45±0.20a	0.90±0.01a	1 560.40±282.32a	1 662.87±251.41a	0.99±0.00b	858.40±187.83ab

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