Effects of Vicia villosa Roth Cultivation on Soil Microbial Community Structure in Apple Orchards

doi:10.7668/hbnxb.20193273

Abstract

Abstract:

In order to determine the effects of V.villosa Roth cultivation in apple orchard on soil microbial community structure,the high-throughput sequencing technology was used to analyze the microbial structure of V.villosa Roth cultivation soil in seven areas of Shandong Province and different phenological period of the same orchard,taking natural grass treatment as comparison.The correlation between microbial community structure and physicochemical properties of grass cultivation soil was analyzed.Results showed that,in seven sampled sites of Shandong Province,the relative abundances of Metarhizium in V.villosa Roth cultivation treatment were all higher than those in the control,and the difference was biggest in Dongying Area.The relative abundances of Fusariumin V.villosa Roth cultivation treatment of six sites were lower than those of the control,except for Zoucheng.The relative abundances of Gibberella in V.villosa Roth cultivation treatment of six sites were lower than those in the control,except for Rongcheng.In Zoucheng and Longkou,the relative abundances of Alternaria were lower than those of the control.Analysis of microbial community structure in different apple phenological stages of V.villosa Roth cultivation in the same orchard showed that,the relative abundance of Aspergillus in flowering stage soil was much lower than that of natural grass.The relative abundances of Fusarium and Aspergillus in fruit setting stage in V.villosa Roth cultivation treatment were lower than those of natural grass.The relative abundance of Bacillus in V.villosa Roth treatment was higher in fruit expansion stage.The relative abundance of Bradyrhizobium was higher in mature stage,while Gibberella,Cladosporium and Bipolaris were much lower than those of natural grass.Effects of V.villosa Roth cultivation in apple orchard on soil microbial community structure differed in different region and phenological stage.However,the relative abundance of beneficial bacteria in soil was increased,while that of pathogenic fungi was decreased,which was helpful to improve orchard soil micro-ecological environment and promote the healthy cultivation of fruit trees.

Key words: V.villosa Roth, Apple orchards, Grass cultivation, Soil microorganism, Community structure

JIANG Lili, SUN Ruihong, ZHANG Ganyu, GONG Qingtao, WU Haibin, DU Xiaokang. Effects of Vicia villosa Roth Cultivation on Soil Microbial Community Structure in Apple Orchards[J]. Acta Agriculturae Boreali-Sinica, 2022, 37(S1): 291-299. doi: 10.7668/hbnxb.20193273.

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Figures/Tables 7

Fig.1 Dilution curve of all treatment

Fig.2 Effects of V.villosa Roth on soil bacterial community structure in orchard of different areas 1.Others;2.Streptomyces;3.Flavobacterium;4.Unidentified_Gammaproteobacteria;5.Bryobacter;6.Flavisolibacter;7.Acidibacter;8.Unidentified_Acidobacteria;9.Unidentified_Chitinophagaceae;10.Unidentified_Anaerolineaceae;11.Chryseolinea.

Fig.3 Effects of V.villosa Roth on soil fungal community structure in orchard of different areas 1.Others;2.Pseudeurotium;3.Solicoccozyma;4.Motierella;5.Gibberella;6.Alternaria;7.Petriella;8.Pseudogymnoascus;9.Metarhizium;10.Neocosmospora;11.Fusarium.

Fig.4 Effects of V.villosa Roth on soil bacterial community structure in different phenological stages in apple orchards 1.Bacillus;2.Arthrobacter;3.TM7a; 4.Segetibacter;5.Massilia;6.Pedobacter;7.Flavisolibacter;8.Edaphobaculum;9.Lysobacter;10.Sphingomonas;11.Ramlibacter;12.Bradyrhizobium;13.Enterococcus;14.Bryobacter;15.Candidatus_Solibacter;16.MND1;17.Haliangium;18.Dongia;19.Reyranella;20.Steroidobacter;21.Acidibacter;22.Microlunatus;23.Mycobacterium;24.Microvirga;25.Blastococcus;26.Subgroup_10; 27.Pantoea;28.Gaiella;29.RB41; 30.Ellin6067; 31.YC-ZSS-LKJ147; 32.Unidentified_Chloroplast; 33.Candidatus_Udaeobacter;34.Gemmatimonas;35.Skermanella.

Fig.5 Effects of V.villosa Roth on soil fungal community structure in different phenological stages in apple orchards 1.Gibberella;2.Cladosporium;3.Diaporthe;4.f_Lasiosphaeriaceae;g_unidentified;5.Hannaella;6. Unidentified_Rozellomycota_sp;7.f_Coniothyriaceae;g_unidentified;8.Bipolaris;9.Unidentified_Mortierellales_sp;10.Acremonium;11.Trichocladium;12.Idriella;13.Unidentified_Mortierellomycotina;14.Fusicolla;15.Cryptococcus;16.Plectosphaerella;17.Unidentified_Ascomycota_sp;18.Chaetomium;19.Fusarium;20.Exobasidium;21.Unidentified_Chytridiomycetes_sp;22.Cystofilobasidium;23.Scytinostroma;24.Unidentified_Glomeromycotina;25.Rhizophlyctis;26.Alternaria;27.Mortierella;28.Solicoccozyma;29.Acrophialophora;30.Darksidea;31.Talaromyces;32.Canariomyces;33.Ectophoma;34.f_Chaetomiaceae;g_unidentified;35.Aspergillus.

Fig.6 Correlation between bacterial relative abundance and physicochemical properties of grass cultivation soil in apple orchard 1.Candidatus_Koribacter; 2.Chujaibacter; 3.Unidentified_Nitrospiraceae; 4.Luteimonas; 5.Ramlibacter; 6.Segetibacter; 7.Flavitalea; 8.Pseudoxanthomonas; 9.Terrimonas; 10.Arenimonas;11.Stenotrophobacter; 12.Bacillus; 13.Ohtaekwangia; 14.Ferruginibacter; 15.Dongia; 16.Haliangium; 17.Arenibacter; 18.Adhaeribacter; 19.Granulicella; 20.Lysobacter; 21.Arthrobacter;22.Steroidobacter; 23.Nocardioides; 24.Streptomyces; 25.Muricauda;26.Rhodanobacter; 27.Flavobacterium; 28.Unidentified_Gammaproteobacteria; 29.Flavisolibacter; 30.Bryobacter; 31.Acidibacter; 32.Unidentified_Chitinophagaceae; 33.Unidentified_Acidobacteria; 34.Chryseolinea;35.Unidentified_Anaerolineaceae.Red indicates positive correlation, blue indicates negative correlation,the same as Fig.7.

Fig.7 Correlation between fungal relative abundance and physicochemical properties of grass cultivation soil in apple orchard 1.Albifimbria; 2.Geomyces; 3.Leucosporidium; 4.Mycena; 5.Dokmaia; 6.Acaulium; 7.Plectosphaerella; 8.Chaetomium; 9.Neonectria; 10.Minimedusa; 11.Trichocladium; 12.Stachybotrys; 13.Thielaviopsis; 14.Talaromyces; 15.Pseudallescheria; 16.Dactylonectria; 17.Trichoderma; 18.Mrakia; 19.Fusicolla; 20.Pseudombrophila; 21.Cladosporium; 22.Gibellulopsis; 23.Coprinus; 24.Cephalotrichum; 25.Mycothermus; 26.Pseudeurotium; 27.Solicoccozyma; 28.Gibberella; 29.Mortierella; 30.Alternaria; 31.Fusarium; 32.Pseudogymnoascus; 33.Neocosmospora; 34.Metarhizium; 35.Petriella.

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