Effects of Organic and Inorganic Nitrogen Fertilizer on Soil Microorganism and Enzyme Activity of Typical Vegetable Soil

doi:10.7668/hbnxb.2017.04.021

Abstract

Abstract: Field experiments were conducted to study the effects of different organic and inorganic nitrogen (N) fertilizers on soil microorganism and enzyme activity of typical vegetable soil for pakchoi and lettuce across three years from 2011 to 2013,using pig manure,biogas manure and pig manure composting as materials,including no fertilizer (CK),no N fertilizer (PK),chemical fertilizer (NPK),organic and inorganic fertilizer 1 (20% pig manure N + 80% chemical fertilizer N,NPKM1),organic and inorganic fertilizer 2 (20% biogas manure N + 80% chemical fertilizer N,NPKM2)and organic and inorganic fertilizer 3 (20% pig manure composting N + 80% chemical fertilizer N,NPKM3). Results showed that soil bacteria number was on the rise,while soil fungi and actinomycetes number appeared to rise first and then fall later during vegetable growing season,and the microbial population in following season were higher than that of previous season. Compared with NPK treatment,NPKM1,NPKM2,NPKM3 treatments increased soil bacteria number by 2.27%-26.16% (Pakchoi),15.06%-40.82% (Lettuce); soil fungi number by 33.62%-47.10% (Pakchoi),39.64%-52.27% (Lettuce); soil actinomyces number by 6.59%-38.21% (Pakchoi),20.97%-29.66% (Lettuce). Organic and inorganic N fertilizer application could promote the reproducing of soil microorganisms in vegetable field. Compared with NPK treatment,NPKM1,NPKM2,NPKM3 treatments increased the activities of soil urease,phosphatase,catalase,and cellulase. Organic and inorganic N fertilizer application could promote the improvement of soil enzyme activity in vegetable field. The yield of each treatment were among 6.73-22.50 t/ha(Pakchoi) and 21.54-47.79 t/ha(Lettuce),and in the range of NPKM3 > NPKM1 > NPKM2 > NPK > PK > CK. Correlation analysis showed that there was a close relationship between soil microorganism quantity,enzyme activity and yield for pakchoi and lettuce. In conclusion,organic and inorganic N application,especially pig manure composting,could effectively improve the soil microbial population and enzyme activities,and maintain high soil fertility plantation,facilitating the sustainable development and efficient production of vegetable.

Key words: Organic and inorganic nitrogen fertilizer, Vegetable soil, Soil microorganism, Soil enzyme activity

CLC Number:

S143.1

TANG Guirong, ZHOU Xuan, TIAN Chang, PENG Huihui, ZHANG Yuping, RONG Xiangmin, LIU Qiang. Effects of Organic and Inorganic Nitrogen Fertilizer on Soil Microorganism and Enzyme Activity of Typical Vegetable Soil[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(4): 129-136. doi: 10.7668/hbnxb.2017.04.021.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2017.04.021

http://www.hbnxb.net/EN/Y2017/V32/I4/129

References

[1] 李世清,李生秀.有机物料在维持土壤微生物体氮库中的作用[J].生态学报,2001,21(1):136-142.
[2] 陈宵宇,周连仁,刘妍.有机无机肥配施对黑土酶活性及作物产量的影响[J].东北农业大学学报,2012,43(2):88-91.
[3] 李东坡,武志杰,陈利军,等.长期培肥黑土脲酶活性动态变化及其影响因素[J].应用生态学报,2003,14(12):2208-2212.
[4] 荣勤雷,梁国庆,周卫,等.不同有机肥对黄泥田土壤培肥效果及土壤酶活性的影响[J].植物营养与肥料学报,2014,20(5):1168-1177.
[5] 盛荣,肖和艾,丁龙君,等.施肥对红壤旱地和稻田土壤可培养微生物和微生物生物量磷的影响[J].农业现代化研究,2010,31(5):626-629.
[6] 万忠梅,宋长春.土壤酶活性对生态环境的响应研究进展[J].土壤通报,2009(4):951-956.
[7] 谢林花,吕家珑,张一平.长期不同施肥对石灰性土壤微生物磷及磷酸酶的影响[J].生态学杂志,2004,23(4):65-68.
[8] 张玉平,刘强,荣湘民,等.不同有机肥与化肥配施对稻田土壤微生物活性的影响[J].土壤通报,2013,44(6):1434-1439.
[9] 鲁艳红,杨曾平,郑圣先,等.长期施用化肥、猪粪和稻草对红壤水稻土化学和生物化学性质的影响[J].应用生态学报,2010,21(4):921-929.
[10] 张树金,余海英,李廷轩,等.温室土壤磷素迁移变化特征研究[J].农业环境科学学报,2010,29(8):1534-1541.
[11] Forge T A,Bittman S,Kowalenko C G. Responses of grassland soil nematodes and protozoa to multi-year and single-year applications of dairy manure slurry and fertilizer[J]. Soil Biology & Biochemistry,2005,37(10):1751-1762.
[12] Yang Z,Singh B R,Sitaula B K. Fractions of organic carbon in soils under different crop rotations,cover crops and fertilization practices[J]. Nutrient Cycling in Agroecosystems,2004,70(2):161-166.
[13] Masakazu A,Naonori K. Quantitative and qualitative changes of organic matter in an Ando soil induced by mineral fertilizer and cattle manure applications for 20 years[J]. Soil Science and Plant Nutrition,2012,47(2):241-252.
[14] 司婷.有机无机肥配施对旱地作物生长的影响及环境效应研究[D].长沙:湖南农业大学,2012.
[15] 李振高,骆永明,滕应.土壤与环境微生物研究法[M].北京:科学出版社,2008.
[16] 任全,单武雄,肖润林,等.不同施肥措施对红壤丘陵茶园土壤酶活性及呼吸强度的影响[J].农业现代化研究,2007,28(4):498-500.
[17] 徐晶,陈婉华,孙瑞莲,等.不同施肥处理对湖南红壤中微生物数量及酶活性的影响[J].土壤肥料,2003(5):8-11.
[18] 徐福利,梁银丽,张成娥,等.施肥对日光温室黄瓜生长和土壤生物学特性的影响[J].应用生态学报,2004,15(7):1227-1230.
[19] 杜社妮,梁银丽,徐福利,等.施肥对日光温室土壤微生物与酶活性变化的影响[J].中国生态农业学报,2007,15(4):68-71.
[20] 高巍,张淑红,张恩平,等.不同培肥方式对菜田土壤微生物生态特征的影响[J].沈阳农业大学学报,2009,40(2):140-143.
[21] 张淑红,高巍,张恩平,等.长期定位施氮对连作番茄土壤可培养微生物数量的影响[J].土壤通报,2012,43(1):60-65.
[22] 叶俊,Perez P G,王小丽,等.不同栽培环境下有机与常规蔬菜土壤的细菌群落多样性差异[J].农业环境科学学报,2012,31(4):728-735.
[23] 钟珍梅,黄秀声,翁伯琦,等.沼液对种植狼尾草的山地红壤可溶性有机氮和土壤微生物特征的影响[J].水土保持学报,2015,29(5):111-116.
[24] Tian L,Dell E,Shi W. Chemical composition of dissolved organic matter in agroecosystems:Correlations with soil enzyme activity and carbon and nitrogen mineralization[J]. Applied Soil Ecology,2010,46(3):426-435.
[25] 和文祥,来航线,武永军,等.培肥对土壤酶活性影响的研究[J].浙江大学学报:农业与生命科学版,2001,27(3):265-268.
[26] 杨丽娟,李天来,付时丰,等.施用有机肥和化肥对菜田土壤酶动态特性的影响[J].土壤通报,2005,36(2):223-226.
[27] 尤彩霞,陈清,任华中,等.不同有机肥及有机无机配施对日光温室黄瓜土壤酶活性的影响[J].土壤学报,2006,43(3):521-523.
[28] 王延军,宗良纲,李锐,等.不同肥料对有机栽培番茄生长和土壤酶及微生物量的影响[J].南京农业大学学报,2007,30(3):83-87.
[29] 李轶,刘庆玉,张玉龙,等.沼肥对保护地土壤酶及其呼吸强度的影响[J].中国土壤与肥料,2007(5):44-47.
[30] 马宁宁,李天来,武春成,等.长期施肥对设施菜田土壤酶活性及土壤理化性状的影响[J].应用生态学报,2010,21(7):1766-1771.
[31] 杨苞梅,李国良,姚丽贤,等.有机肥施用模式对蔬菜产量、品质及土壤酶活性的影响[J].土壤通报,2011,42(1):70-76.
[32] 周崇峻,李凤巧,李明静,等.有机肥和化肥配施对温室黄瓜土壤酶活性的影响[J].沈阳农业大学学报,2013,44(5):634-638.
[33] 王文锋,李春花,黄绍文,等.不同施肥模式对设施菜田土壤酶活性的影响[J].应用生态学报,2016,27(3):873-882.
[34] 王文锋,李春花,黄绍文,等.不同施肥模式对设施秋冬茬芹菜生育期间土壤酶活性的影响[J].植物营养与肥料学报,2016,22(3):676-686.

Please choose a citation manager

Content to export