Effect of Nitrification Inhibitor and Fertilizer Reduction on Reducing Nitrogen Source Gas Emission and Their Interaction with Microbial Functional Genes in Greenhouse Vegetable Field

doi:10.7668/hbnxb.20191952

Abstract

Abstract: It was carried out this experiment to reduce nitrogen source gas emission and improve fertilizer efficiency in greenhouse vegetable field. By setting up a comparison among conventional fertilization of farmers pattern, fertilizer reduction and different nitrification inhibitors in the greenhouse cucumber soil, the gas emission regularity of nitrogen source and yields were monitored with different fertilization treatments for five years. Based on qPCR when compared with conventional fertilization of farmers treatment (FT) and fertilizer reduction treatment (OPT). The total annual emission of nitrous oxide (N₂O) reduced 28.4% and 21.8% respectively by fertilizer reduction (OPT) and applying nitrification inhibitor DCD (In-1) compared with conventional fertilization (FT).The interaction between nitrogen oxides (NO_x) emission and ammonia-oxidizing archaea was analyzed. On the high-throughput sequencing Illumina MiSeq platform, metagenomes were predicted from 16S rDNA gene sequence data using PICRUSt, and functional genes associated with nitrogen metabolisms were identified based on KEGG database. The results showed that the total annual emission of nitrogen oxides (NO_x) by applying nitrification inhibitor DCD (treatment In-1) was respectively reduced by 76.5% and 71.8%. It was found that DCD (In-1) significantly inhibited Ammonia-oxidizing archaea (AOA) population, and the effect was better than pyridine (In-2) in calcareous cinnamon soil in the North greenhouse. There was a positive exponential correlation between the gene copy numbers of AOA and soil NO_x flux. The equation was as follows:Y=121.18e^3E-08x (R ²=0.840 5, P<0.05). The identification results based on KEGG database showed that the relative abundance of enoyl-CoA hydratase(K01692), substrate-binding protein(K02035), transport system ATP-binding protein(K09687) functional genes associated with assimilatory pathways was higher than others. The three functional genes metabolic pathways in soil were the most sensitive nitrogen cycle pathways responding to the variation with the regulation of nitrification inhibitors and fertilizer reduction. In five years,compared with conventional fertilization of farmers' treatment (FT), the partial factor productivity of fertilizer with fertilizer reduction treatment (OPT) and DCD treatment (In-1) increased by 32.1%-38.2% and 17.8%-21.9% respectively. It was suggested that application of nitrification inhibitors (weight of 15% N) and fertilizer reduction (25%) effectively reduced nitrogen source gas emission and improved fertilizer utilization rate in the North greenhouse cucumber soil. It was found that the emission flux of NO_x increased sharply with the increase of AOA population. This study provided a strong scientific basis for the effective prevention and control of agricultural non-point source pollution and the promotion of high quality and green development of agriculture.

Key words: Greenhouse vegetable soil, Reducing nitrogen emission, Ammonia-oxidizing archaea, Nitrification inhibitor, Microbial functional genes

CLC Number:

S154.36
S19

WANG Ling, ZHANG Guoyin, LIU Mengchao, SUN Shiyou, LIU Lei, RU Shuhua, ZHAO Ouya, LI Bowen. Effect of Nitrification Inhibitor and Fertilizer Reduction on Reducing Nitrogen Source Gas Emission and Their Interaction with Microbial Functional Genes in Greenhouse Vegetable Field[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(2): 196-203. doi: 10.7668/hbnxb.20191952.

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