Preliminary Study of Chestnut Fungus Community During Storage

doi:10.7668/hbnxb.20190647

Abstract

Abstract: To verify the diversity of fungal community structure in chestnut during storage, the fungal ITS amplitons of chestnut shell and pip from 6 species was sequenced by high-throughput method. It was found that the main pathogen of different chestnut variety differed greatly. Few were found in ancient chestnut in Mata village, Zhuyang town. Ancient chestnut in Tonghuagou village, Zhuyang town was Gibberella. Ancient chestnut in Dayu village, Huangqian town was Fusarium and Botryosphaeria. Ancient chestnut in Zhuanwayao, Xiagang town was Penicillium. Ancient chestnut in Honghe village, Jiaoyu town was Trichoderma. Castanea mollissima cv. Zaofeng was Penicillium and Colletotrichum. Community diversity index analyze showed that, fungus diversity, dominance, abundance and phylogenetic diversity of ancient chestnut in Mata village were the highest. Analysis within species found that, for ancient chestnut in Mata village, zhuyang town, the abundance of Fusarium in pip was significantly lower than that in shell. For ancient chestnut in Tonghuagou village, Zhuyang town, the abundance of Gibberella in pip was significantly lower than that in shell. For ancient chestnut in Honghe village, Jiaoyu town, the abundance of Penicillium in pip was significantly higher than that in shell. For C. mollissima cv. Zaofeng, the abundance of Penicillium in pip was significantly higher than that in shell, and the abundance of Colletotrichum was significantly lower than that in shell. At the same time, the fungal community structure of chestnut pip and shell was highly similar for ancient chestnut in Mata village, Zhuyang town, ancient chestnut in Dayu village, Huangqian town and C. mollissima cv. Zaofeng; and the fungal community structure of chestnut pip and shell differed greatly for ancient chestnut in Honghe village, Jiaoyu town and ancient chestnut in Zhuanwayao, Xiagang town.

Key words: Castanea mollissima, Fruit rot, Variety, Microbial diversity, Community structure

CLC Number:

S432.4

JIANG Lili, WU Haibin, GONG Qingtao, LIU Qinglian, SUN Ruihong. Preliminary Study of Chestnut Fungus Community During Storage[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(S1): 302-308. doi: 10.7668/hbnxb.20190647.

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