Analysis of Bacterial Community Structure and Diversity in Rhizosphere Soil of Different Fruit Vegetables in Greenhouse Continuous Cropping on High-throughput Sequencing

doi:10.7668/hbnxb.20192021

Abstract

Abstract: In order to reveal the changes of bacterial community structure and diversity in rhizosphere soil of different fruit vegetables under greenhouse continuous cropping, the 16S rRNA gene of bacteria from the rhizosphere soil of planting tomato, cucumber, pepper and eggplant under continuous cropping for five years in a row and the open field as control, was sequenced by high throughput sequencing, and explore the effects of different crops in protected continuous cropping on soil bacterial community structure and diversity.The results showed that there were 44 phylum, 150 classes, 364 orders, 575 families and 1 046 genus of bacteria in greenhouse continuous cropping soil. The 6 phylum with high abundance were Proteobacteria, Chloroflexi, Acidobacteriota, Actinobacteriota, Bacteroidota and Gemmatimonadota, which accounted for 84.56%of the total bacterial community in open field, 83.02% in tomato, 82.21% in cucumber, 80.67% in pepper and 79.25% in eggplant.The abundance of dominant bacteria in rhizosphere soil between four different crops showed a significant difference, and all of them were lower than open field.There were the highest Protrobacteria with 31.53% of the total bacterial communities and the lowest A ctionbacteriota with 4.95% after continuous cropping of cucumber, that showed the dominant bacterial population in rhizosphere soil of planting cucumber was higher than other vegetables and open field.While the soil quality of continuous cropping tomato, that the abundance of Proteobacteria was the highest and Acidobacteriota was the lowest, accounting for 23.56% and 5.16% of the total bacterial community, respectively.There was no significant difference in the bacterial community diversity of rhizosphere soil among the four continuous cropping vegetables, but there was significant difference between continuous cropping cucumber and open field. Which showed that the diversity of rhizosphere microorganisms in different crops was no significant difference.It would provide a theoretical basis for continuous cropping of different fruit vegetables and a theoretical basis for agricultural sustainable cultivation.

Key words: Fruit vegetables, Greenhouse continuous cropping, High-throughput sequencing, Microbial diversity

CLC Number:

S432.4

JIANG Wei, BAI Hongmei, XUE Guoping, DU Jinwei, GAO Jing, LI Jie, MU Zongjie, HU Yuzhen, SONG Qingcheng. Analysis of Bacterial Community Structure and Diversity in Rhizosphere Soil of Different Fruit Vegetables in Greenhouse Continuous Cropping on High-throughput Sequencing[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(4): 82-89. doi: 10.7668/hbnxb.20192021.

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