Cloning of TabZIP2 Gene and Expression Analysis under Low Temperature and ABA in Winter Wheat Dn1

doi:10.7668/hbnxb.20191127

Abstract

Abstract: Winter wheat Dongnongdongmai 1(Dn1) is the first strong cold-resistant variety that can safely overwinter in Heilongjiang Province. At present, many related cold resistance genes have been studied. To explore the effect of exogenous ABA on the winter wheat TabZIP2 gene under low temperature stress, used Dn1 as experimental material, the full length of CDS sequence of TabZIP2 gene was obtained by PCR, and used bioinformatics to predict it. Later, we used the weak cold-resistant winter wheat variety Jimai 22(JM22) as comparative material, the tissue stress analysis of TabZIP2 gene was carried out by qRT-PCR, to investigate the effect of ABA on the relative expression of TabZIP2 in tiller node and leaves of Dn1 at different temperatures. Bioinformatics analysis results showed that:TabZIP2 contained a complete open reading frame of 759 bp, the TabZIP2 gene-encoded protein consisted of 252 amino acids and was an unstable protein with a molecular weight of 26.595 6 ku;Secondary structure prediction α-helix(H) and random coils(C) account for the majority;The protein had no transmembrane region and signal peptide, and was located in the nucleus with strong hydrophilic ability, and TabZIP2 was closely related to Brachypodium serrata and rice, the protein had a conserved basic leucine zipper domain. The results of expression analysis showed that Dn1 leaves responded to low temperature stress earlier than tillers under cold stress;TabZIP2 expression in tiller node of Dn1 and leaves was significantly higher than that in JM22;ABA could promote the increase of TabZIP2 expression in tiller nodes and leaved at-10, -25℃, preliminary judgment that ABA could improve cold resistance of winter wheat. This study preliminarily explored the function of TabZIP2 in the cold resistance of Dn1, and also laid a theoretical foundation for the regulation mechanism of ABA signal at low temperature.

Key words: Winter wheat, TabZIP2, Low temperature, ABA, Bioinformatics

CLC Number:

Q94
S512.03

GUAN Ying, LIU Nan, CANG Jing, WEI Tiesuo, WU Jingjing, QI Yue, GUO Fuye, LI Yuanshan. Cloning of TabZIP2 Gene and Expression Analysis under Low Temperature and ABA in Winter Wheat Dn1[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(4): 75-81. doi: 10.7668/hbnxb.20191127.

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