Cloning and Functional Analysis of the Molybdenum Cofactor Sulfurase Gene(LOS5)in Potato

doi:10.7668/hbnxb.20196209

Abstract

Abstract:

To investigate the structure and function of the potato molybdenum cofactor sulfurase(LOS5)gene,StLOS5 was cloned from the potato cultivar Zicai No.3 using PCR,followed by bioinformatics analysis,subcellular localization assays,yeast stress-tolerance assays,and expression pattern analysis under drought stress.The results showed that the CDS region of the potato StLOS5 gene was 2 460 bp in length,encoding 819 amino acids,with a molecular weight of 91.50 ku,a theoretical isoelectric point of 6.78,and a GRAVY value of -0.268,indicating that the protein was hydrophilic.Phylogenetic analysis revealed that the StLOS5 protein had the highest homology with tomato LOS5,suggesting that the two proteins may possess similar functions.Multiple cis-acting elements related to abiotic stress were detected in the promoter region.Subcellular localization showed that StLOS5 was primarily localized in the nucleus.Yeast stress-tolerance assays indicated that StLOS5 enhances tolerance to osmotic stress.Quantitative Real-time PCR analysis demonstrated that StLOS5 exhibits tissue-specific expression,with higher transcript levels in leaves,and responds rapidly to drought stress,with its expression in leaves peaking 12 h after treatment with 8%PEG.These results indicated that StLOS5 plays a role in the potato response to drought stress.

Key words: Potato, StLOS5, Bioinformatics analysis, Drought stress

CLC Number:

WANG Yingqi, LI Jingwei, YU Zhuo, ZHAO Jinbang, YUAN Qinghua, LIU Bo, JIANG Chao. Cloning and Functional Analysis of the Molybdenum Cofactor Sulfurase Gene(LOS5)in Potato[J]. Acta Agriculturae Boreali-Sinica, 2026, 41(1): 56-63. doi: 10.7668/hbnxb.20196209.

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References 38

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名称 Name	序列 Sequence	数量 Number	功能 Function
AAGAA-MOTIF	AAGAA	2	生长发育相关元件
ABRE	AGGTG	1	脱落酸响应元件
AC-Ⅱ	ACCAACC	1	脱落酸响应元件
ARE	TGGTTT	3	厌氧诱导元件
BOX-4	ATTAAT	3	光响应元件
CTAG-MOTIF	GATC	1	光响应元件
ERE	ATTTCAAA	3	乙烯响应元件
G-BOX	CACGTG	1	光响应元件
GC-MOTIF	CCCCCG	1	缺氧诱导元件
GT1-MOTIF	GGTTAA	2	光响应元件
W BOX	TTGAC	1	病原/防御相关元件
WRE3	CCACCT	2	损伤诱导元件
WUN-MOTIF	AAATTTCCT	1	创伤反应元件
LTR	CCGAA	1	低温响应元件
MYB	CAACTG	4	MYB类转录因子结合位点(主要参与干旱、盐分、ABA等胁迫应答)
MYB-LIKE SEQUENCE	TAACCA	1	MYB 类转录因子结合位点(光/激素应答模块)
MYC	CACATG	1	MYC 类转录因子结合位点(参与 ABA 介导的胁迫响应)
STRE	AGGGG	2	非特异性逆境响应元件
TATA-BOX	TATA	57	转录起始点核心启动子元件
TCA	TCAGAAGAGG	1	水杨酸响应元件
I-BOX	GATAAGG	1	光响应元件

名称 Name	序列 Sequence	数量 Number	功能 Function
AAGAA-MOTIF	AAGAA	2	生长发育相关元件
ABRE	AGGTG	1	脱落酸响应元件
AC-Ⅱ	ACCAACC	1	脱落酸响应元件
ARE	TGGTTT	3	厌氧诱导元件
BOX-4	ATTAAT	3	光响应元件
CTAG-MOTIF	GATC	1	光响应元件
ERE	ATTTCAAA	3	乙烯响应元件
G-BOX	CACGTG	1	光响应元件
GC-MOTIF	CCCCCG	1	缺氧诱导元件
GT1-MOTIF	GGTTAA	2	光响应元件
W BOX	TTGAC	1	病原/防御相关元件
WRE3	CCACCT	2	损伤诱导元件
WUN-MOTIF	AAATTTCCT	1	创伤反应元件
LTR	CCGAA	1	低温响应元件
MYB	CAACTG	4	MYB类转录因子结合位点(主要参与干旱、盐分、ABA等胁迫应答)
MYB-LIKE SEQUENCE	TAACCA	1	MYB 类转录因子结合位点(光/激素应答模块)
MYC	CACATG	1	MYC 类转录因子结合位点(参与 ABA 介导的胁迫响应)
STRE	AGGGG	2	非特异性逆境响应元件
TATA-BOX	TATA	57	转录起始点核心启动子元件
TCA	TCAGAAGAGG	1	水杨酸响应元件
I-BOX	GATAAGG	1	光响应元件

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