Abstract:

The objective of this research was to examine the structure and physicochemical characteristics of the ribosomal protein L8 gene (RPL8) in Sangsang yak,and to assess the expression of RPL8 in various tissues via qPCR,providing a theoretical foundation for further studies on the biological functions of RPL8 in yak.The RPL8 gene coding region (CDS) of Sangsang yak was cloned using kidney tissue cDNA as a template,and the gene sequence was analyzed using bioinformatics.RT-qPCR was employed to determine the relative expression level of RPL8 in different tissues of Sangsang yak.The CDS region of RPL8 gene in Sangsang yak was 456 bp,encoding 151 amino acids.Comparative analysis revealed that Sangsang yak had the highest homology with domestic cattle and the lowest similarity with American bear.Protein analysis identified 18 potential phosphorylation sites in RPL8 protein,molecular weight of 16.293 89 ku,total number of atoms of 2 325,amino acid isoelectric point of 10.90,instability coefficient of 41.81,and total average hydrophobicity of -0.687.The protein was predicted to be an unstable hydrophilic protein without transmembrane domains or signal peptide regions.Subcellular localization demonstrated that RPL8 protein was primarily located in the nucleus (65.20%).The secondary structure of RPL8 protein was composed of random coil (49.01%),extension chain (27.15%),β-corner (13.91%),and α-helix (9.93%).RT-qPCR results indicated that RPL8 gene was expressed in all 8 tissues of Sangsang yak,with the highest expression in heart,liver,and duodenal tissues,exhibiting significant differences compared to other tissues.

Key words: Sangsang yak, RPL8 gene, Gene cloning, Bioinformatics analysis, Tissue expression

CLC Number: 

• S823.85