Abstract:

To investigate the structure and function of the PPP1R11 gene in yaks,along with its expression pattern in eight distinct tissues,cDNA from the testicular tissue of Pamir yaks was utilized as the template,the CDS region sequence of the PPP1R11 gene in Pamir yaks was cloned via PCR technology,followed by bioinformatics analysis.The expression level of the PPP1R11 gene in eight tissues of the Pamir yak was determined using Real-time Fluorescence Quantitative PCR(RT-qPCR).The results showed that the coding sequence(CDS region)of PPP1R11 gene in Pamir yak was 324 bp and encoded 107 amino acids.The homology comparison showed that Pamir yak had the highest similarity with Bos mutus(100%)and the lowest similarity with Mus musculus(84.6%).The results of bioinformatics analysis showed that the molecular formula of PPP1R11 protein was C₅₀₃H₈₀₃N₁₆₃O₁₆₄S₈,the total number of atoms was 1 641,the instability index and the total average hydrophilic index were 65.91 and -1.385,respectively.PPP1R11 protein had no signal peptide and transmembrane structure,and was mainly located in the nucleus.The protein had one glycosylation site and 19 potential phosphorylation sites.The secondary structure of the protein was mainly random curling,and it mainly interacted with PPP1R7,PPP1CA and NSFL1C.The RT-qPCR results showed that PPP1R11 gene was expressed differently in heart,muscle,fat,spleen,liver,pancreas,kidney and testis of Pamir yak,and the expression level of PPP1R11 gene was significantly higher in testis than in the other seven tissues.

Key words: Pamir yak, PPP1R11 gene, Gene cloning, Tissue expression

CLC Number: 

• S823

• Q78基因工程(遗传工程)