QTL Mapping and Gene Functional Annotation for Controlling Maize Leaf Number

doi:10.7668/hbnxb.20195943

Abstract

Abstract:

To clarify the genetic characteristics of total leaf number and leaf number above the ear in maize,and reveal their regulatory effects on maize yield potential,biomass accumulation,and lodging resistance,experiments on QTL mapping and gene mining for controlling maize leaf number were conducted.By using SLAF-seq technology for high-throughput sequencing of Chang 7-2,PHB1M,and 138 F_2∶3 families,combined with two planting density treatments(E1:60 000 plants/ha;E2:120 000 plants/ha)QTL mapping was performed on leaf number phenotypic data,and gene mining was performed on the mapping results.The results showed that two total leaf number QTLs distributed on chromosome 8 were the main effect QTLs,contribution rates were 16.96% and 23.08%,respectively,and the additive effect value was negative;the QTL for the leaf number above the ear distributed on chromosomes 2 and 4,with a positive additive effect value.the two QTLs distributed on chromosome 4 were the main effect QTL,contribution rates were 10.18% and 14.75%,respectively;the QTLs for total leaf number and leaf number above the ear were distributed in different chromosomal regions and might be subject to relatively independent genetic regulation.Gene functional analysis revealed genes screened involvement in carbohydrate metabolism,plant hormone signal transduction,and selected some transcription factors.The results of this study provide richer theoretical support for further revealing the genetic basis of maize leaf number and offer targets for marker-assisted breeding.

Key words: Maize, Total leaf number, Leaf number above the ear, QTL, Gene mining

CLC Number:

XUE Chunlei, ZHANG Xuting, ZHANG Hailong, FU Zengjuan, LIU Yanan, WU Haiyan, ZHANG Ziyu, ZHANG Sainan, YU Zhonghao, WU Hui, HAN Pingan, MA Yan, WANG Yongxing, SUN Fengcheng. QTL Mapping and Gene Functional Annotation for Controlling Maize Leaf Number[J]. Acta Agriculturae Boreali-Sinica, 2026, 41(1): 49-55. doi: 10.7668/hbnxb.20195943.

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References 29

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样品 Sample	指标 Indicator	总叶片数 Total number of leaf		穗上叶片数 Leaf number above the ear
样品 Sample	指标 Indicator	E1	E2	E1	E2
亲本	昌7-2	21.86±0.35Aa	21.41±0.13Aa	4.78±0.27Aa	4.61±0.33Aa
Parents	PHB1M	18.17±0.62Cc	18.11±0.27Cc	4.94±0.49Aa	5.00±0.00Aa
	均值	20.16±1.09Bb	20.11±1.07Bb	5.23±0.59Aa	5.28±0.61Aa
	偏度	0.20	-0.17	0.14	0.38
	峰度	-0.51	-0.15	0.05	0.44
F_2∶3家系	最小值	18.17	17.33	4.00	4.00
F_2∶3 family	最大值	22.83	22.33	6.67	7.00
	变异系数/%	5.41	5.32	11.28	11.55

样品 Sample	指标 Indicator	总叶片数 Total number of leaf		穗上叶片数 Leaf number above the ear
样品 Sample	指标 Indicator	E1	E2	E1	E2
亲本	昌7-2	21.86±0.35Aa	21.41±0.13Aa	4.78±0.27Aa	4.61±0.33Aa
Parents	PHB1M	18.17±0.62Cc	18.11±0.27Cc	4.94±0.49Aa	5.00±0.00Aa
	均值	20.16±1.09Bb	20.11±1.07Bb	5.23±0.59Aa	5.28±0.61Aa
	偏度	0.20	-0.17	0.14	0.38
	峰度	-0.51	-0.15	0.05	0.44
F_2∶3家系	最小值	18.17	17.33	4.00	4.00
F_2∶3 family	最大值	22.83	22.33	6.67	7.00
	变异系数/%	5.41	5.32	11.28	11.55

性状 Traits	染色体 Chr.	QTL	区域/cM Genomic locations of QTL		最大LOD值 Max LOD value	加性效应 Additive effect	贡献率/% Contribution rate
性状 Traits	染色体 Chr.	QTL	起始 Start	终止 End	最大LOD值 Max LOD value	加性效应 Additive effect	贡献率/% Contribution rate
总叶片数	8	qLC-1-LG8	54.848	55.213	7.176	-0.423	16.96
LC	8	qLC-2-LG8	59.271	59.636	8.960	-0.473	23.08
穗上叶片数	2	qLCAE-1-LG2	40.867	41.235	3.509	0.155	8.84
LA	4	qLCAE-1-LG4	76.843	77.208	3.442	0.166	10.18
	4	qLCAE-2-LG4	93.206	93.206	6.821	0.210	14.75

性状 Traits	染色体 Chr.	QTL	区域/cM Genomic locations of QTL		最大LOD值 Max LOD value	加性效应 Additive effect	贡献率/% Contribution rate
性状 Traits	染色体 Chr.	QTL	起始 Start	终止 End	最大LOD值 Max LOD value	加性效应 Additive effect	贡献率/% Contribution rate
总叶片数	8	qLC-1-LG8	54.848	55.213	7.176	-0.423	16.96
LC	8	qLC-2-LG8	59.271	59.636	8.960	-0.473	23.08
穗上叶片数	2	qLCAE-1-LG2	40.867	41.235	3.509	0.155	8.84
LA	4	qLCAE-1-LG4	76.843	77.208	3.442	0.166	10.18
	4	qLCAE-2-LG4	93.206	93.206	6.821	0.210	14.75

不同环境 Different environments	基因名称 Gene name	功能 Function	代谢通路 Metabolic pathways
E1	Chr8_pilon_122057.9_2794.mRNA4	磷酸甘油酸激酶	碳水化合物代谢、糖酵解(map00010)
	Chr8_pilon_125194.9_8713.mRNA5	海藻糖6-磷酸合酶	碳水化合物代谢、淀粉合成(map00500)
	Chr8_pilon_123099.8_2550.mRNA1	生长素相关GH3基因	植物激素信号转导
	Chr8_pilon_121912_708.mRNA1	bZIP	转录因子
	Chr8_pilon_124422.5_1941.mRNA1	AP2/EREBP
	Chr8_pilon_122255.3_702.mRNA2	乙烯响应转录因子
	Chr8_pilon_124355.7_2508.mRNA1	TCP
	Chr8_pilon_125397.6_672.mRNA1	WRKY
	Chr8_pilon_125429.1_4893.mRNA5	丝氨酸/苏氨酸蛋白激酶
	Chr8_pilon_123212_8668.mRNA4
	Chr8_pilon_123983.6_74879.mRNA6
	Chr8_pilon_122773.5_2351.mRNA3
E2	Chr8_pilon_131114.5_2800.mRNA5	MYB	转录因子
	Chr8_pilon_132220_6675.mRNA2
	Chr8_pilon_129770.3_670.mRNA1	HD-Zip
	Chr8_pilon_129595.4_2031.mRNA1	谷胱甘肽转移酶
	Chr8_pilon_131003.1_337.mRNA1	蛋白磷酸酶2C

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