QTL Mapping and Candidate Gene Analysis of the First Flower Node in Kenaf

doi:10.7668/hbnxb.20196050

Abstract

Abstract:

In order to determine the genetic law of the first flower node in kenaf,an F₂ population consisting of 148 individual plants was developed from the inbred lines K215 and K89,which showed significant differences in the first flower node position.The high-density genetic linkage map of kenaf was constructed using RAD-seq for bin marker genotyping.The map contained 3 418 bin markers unevenly distributed on 18 chromosomes,with a total genetic distance of 864.61 cM and an average genetic distance between markers of 0.25 cM.Based on the phenotypic data and genetic map of the first flower node,the first flower node QTL named FFN6 was detected using an interval mapping method.This QTL was located on chromosome 6,with a LOD value of 8.66,a phenotypic contribution rate of 13.77%,and an additive effect value of -19.97.The results indicated that the allele locus comes from the paternal K215 and had a negative regulatory effect on the first flower node.Based on the gene annotation information within the QTL mapping interval,seven candidate genes were preliminarily screened and obtained.The qRT-PCR results showed that under short-day conditions,the expression levels of Hc-bHLH6 and Hc-MYB6 genes significantly increased.The function of the two genes promotes the flowering of kenaf plants,which may be closely related to the node of the first flower of kenaf plants.

Key words: Kenaf, QTL, First flower node, Genetic linkage map

CLC Number:

LI Hui, CHEN Anguo, TANG Huijuan, LUAN Mingbao. QTL Mapping and Candidate Gene Analysis of the First Flower Node in Kenaf[J]. Acta Agriculturae Boreali-Sinica, 2026, 41(3): 75-81. doi: 10.7668/hbnxb.20196050.

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References 28

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引物名称 Primer name	正向序列(5'-3') Forward sequence(5'-3')	反向序列(5'-3') Reverse sequence(5'-3')
bHLH	GCAGTTCCTCTCAATGAAGTTAGC	GATTTTGCTGGGACTGGTGC
MYB	GGGAAGAACAGATAATGAGGTGATG	GTCGGTGGGGTTAGGAGGAA
Hc-actin	TTGCAGACCGTATGAGCAAG	ATCCTCCGATCCAGACACTG

引物名称 Primer name	正向序列(5'-3') Forward sequence(5'-3')	反向序列(5'-3') Reverse sequence(5'-3')
bHLH	GCAGTTCCTCTCAATGAAGTTAGC	GATTTTGCTGGGACTGGTGC
MYB	GGGAAGAACAGATAATGAGGTGATG	GTCGGTGGGGTTAGGAGGAA
Hc-actin	TTGCAGACCGTATGAGCAAG	ATCCTCCGATCCAGACACTG

染色体 Chromosome	SNP 标记数量 No. of SNP marker	bin 标记数量 No. of bin marker	连锁群长度/cM Linkage distance	平均遗传距离/cM Average inter-loci distance	图谱空隙/cM Map gap
LG1	516	126	75.56	0.60	10.59
LG2	3 275	707	119.16	0.17	7.16
LG3	765	172	53.95	0.31	5.68
LG4	1 585	327	83.78	0.26	5.54
LG5	296	43	23.60	0.55	6.78
LG6	6 311	1 085	63.71	0.06	1.83
LG7	220	47	45.26	0.96	12.20
LG8	233	57	40.06	0.70	12.23
LG9	81	17	22.40	1.32	9.41
LG10	103
LG11	1 289	302	114.77	0.38	7.46
LG12	679	157	43.38	0.28	3.57
LG13	347	67	24.26	0.36	4.19
LG14	236	50	26.07	0.52	7.46
LG15	414	128	68.28	0.53	8.37
LG16	396	89	27.28	0.31	4.09
LG17	132	11	2.64	0.24	0.70
LG18	162	33	30.46	0.92	12.06
总计 Total	10 740	3 418	864.61

染色体 Chromosome	SNP 标记数量 No. of SNP marker	bin 标记数量 No. of bin marker	连锁群长度/cM Linkage distance	平均遗传距离/cM Average inter-loci distance	图谱空隙/cM Map gap
LG1	516	126	75.56	0.60	10.59
LG2	3 275	707	119.16	0.17	7.16
LG3	765	172	53.95	0.31	5.68
LG4	1 585	327	83.78	0.26	5.54
LG5	296	43	23.60	0.55	6.78
LG6	6 311	1 085	63.71	0.06	1.83
LG7	220	47	45.26	0.96	12.20
LG8	233	57	40.06	0.70	12.23
LG9	81	17	22.40	1.32	9.41
LG10	103
LG11	1 289	302	114.77	0.38	7.46
LG12	679	157	43.38	0.28	3.57
LG13	347	67	24.26	0.36	4.19
LG14	236	50	26.07	0.52	7.46
LG15	414	128	68.28	0.53	8.37
LG16	396	89	27.28	0.31	4.09
LG17	132	11	2.64	0.24	0.70
LG18	162	33	30.46	0.92	12.06
总计 Total	10 740	3 418	864.61

染色体 Chromosome	基因编号 Gene ID	基因功能注释 Annotation
6	Hca.06G0027130	MYB6转录因子
6	Hca.06G0027530	蔗糖转运蛋白
6	Hca.06G0027640	E3泛素蛋白连接酶
6	Hca.06G0027830	葡萄糖-6-磷酸脱氢酶
6	Hca.06G0028110	生长素相关蛋白
6	Hca.06G0028380	bHLH6转录因子
6	Hca.06G0028430	丝氨酸/苏氨酸蛋白激酶

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