QTL Mapping Analysis of Grain Related Traits of Wheat Based on 55K Chip

doi:10.7668/hbnxb.20194667

Abstract

Abstract:

In order to further explore the primary QTL loci for grain-related traits in wheat and explore the genetic relationships among grain traits,124 DH populations constructed from wheat varieties AN859 and WN988 with large differences in grain traits were utilized as research materials,The phenotypic values of grain length,grain width,and thousand grains weight were measured in seven environments over two years,respectively,to carry out the multiple regression analysis of grain traits,and QTL detection of grain-related traits was performed based on the 55K microarray data of the DH populations.The results showed that grain width contributed most to thousand grains weight in the multiple regression analysis.QTL localization for grain traits by complete interval mapping,a total of 69 QTLs related to grain traits were detected on 19 chromosomes except chromosome 6D and 7B,including 24 QTLs for thousand grains weight,28 QTLs for grain length,and 17 QTLs for grain width,with phenotypic interpretations of individual QTLs ranging from 6.87% to 27.74%.Among them,grain length-associated Qgl.ahau-7A.1 on chromosome 7A was detected under seven environments and BLUP,with a phenotypic interpretation rate of 9.48%—22.26%,an additive effect of 0.11—0.21 mm,and a physical interval of 4.91 Mb(AX-110430243—AX-110442528),for the new primary effector QTL.Therefore,the Qgl.ahau-7A.1 locus can be used as a region of focus for subsequent fine localization and molecular marker-assisted breeding.

Key words: Wheat, Grain trait, Thousand grains weight, Grain length, Grain width, QTL

ZHANG Jinjin, ZHAO Xiaoxue, LIU Ping, WAN Jiale, CHEN Xinyi, CHEN Can, SI Hongqi, LI Liang, MA Chuanxi, LU Jie. QTL Mapping Analysis of Grain Related Traits of Wheat Based on 55K Chip[J]. Acta Agriculturae Boreali-Sinica, 2024, 39(3): 8-14. doi: 10.7668/hbnxb.20194667.

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References 23

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项目 Project	染色体 Chromosome	标记数 Marker number	遗传长度/cM Genetic length	最大间距/cM Max interval	平均间距/cM Average map distance
第一同源群	1A	78	335.86	91.59	4.31
First homology group	1B	51	348.24	35.77	6.83
	1D	57	405.45	58.92	7.11
第二同源群	2A	58	496.02	49.43	8.55
Second homology group	2B	126	593.43	69.68	4.71
	2D	102	774.45	46.62	7.59
第三同源群	3A	147	667.57	56.79	4.54
Third homology group	3B	100	511.90	56.87	5.12
	3D	81	762.34	70.38	9.41
第四同源群	4A	53	316.60	29.43	5.97
Fourth homology group	4B	83	275.36	17.95	3.32
	4D	79	398.41	51.87	5.04
第五同源群	5A	118	703.43	56.34	5.96
Fifth homology group	5B	119	483.09	54.49	4.06
	5D	106	536.13	28.30	5.06
第六同源群	6A	42	262.04	25.85	6.24
Sixth homology group	6B	82	298.97	33.90	3.65
	6D	63	334.41	44.91	5.31
第七同源群	7A	142	665.11	81.35	4.68
Seventh homology group	7B	104	355.99	31.79	3.42
	7D	145	932.52	73.37	6.43
总计Total		1 936	10 457.32	91.59	5.40

项目 Project	染色体 Chromosome	标记数 Marker number	遗传长度/cM Genetic length	最大间距/cM Max interval	平均间距/cM Average map distance
第一同源群	1A	78	335.86	91.59	4.31
First homology group	1B	51	348.24	35.77	6.83
	1D	57	405.45	58.92	7.11
第二同源群	2A	58	496.02	49.43	8.55
Second homology group	2B	126	593.43	69.68	4.71
	2D	102	774.45	46.62	7.59
第三同源群	3A	147	667.57	56.79	4.54
Third homology group	3B	100	511.90	56.87	5.12
	3D	81	762.34	70.38	9.41
第四同源群	4A	53	316.60	29.43	5.97
Fourth homology group	4B	83	275.36	17.95	3.32
	4D	79	398.41	51.87	5.04
第五同源群	5A	118	703.43	56.34	5.96
Fifth homology group	5B	119	483.09	54.49	4.06
	5D	106	536.13	28.30	5.06
第六同源群	6A	42	262.04	25.85	6.24
Sixth homology group	6B	82	298.97	33.90	3.65
	6D	63	334.41	44.91	5.31
第七同源群	7A	142	665.11	81.35	4.68
Seventh homology group	7B	104	355.99	31.79	3.42
	7D	145	932.52	73.37	6.43
总计Total		1 936	10 457.32	91.59	5.40

性状 Trait	环境 Environments	亲本 Parents		DH群体(124份) DH population (124 copies)							遗传力 H²
性状 Trait	环境 Environments	安农 859 AN859	武农988 WN988	最小值 Min	最大值 Max	均值 Mean	标准差 s	偏度 Skew.	峰度 Kurtosis	变异系数/% CV	遗传力 H²
千粒质量/g	E1	34.01	44.25	31.18	61.17	45.58	5.78	-0.18	0.08	12.67	0.87
Thousand	E2	42.50	59.72	32.96	68.76	49.57	6.55	0.18	0.07	13.22
grains	E3	45.11	61.26	40.89	67.96	52.62	5.87	0.27	-0.55	11.15
weight	E4	37.94	42.47	30.83	57.42	42.46	4.89	-0.02	0.17	11.49
	E5	47.96	59.44	38.74	67.64	50.35	5.53	0.33	-0.17	10.98
	E6	47.99	54.34	36.93	62.19	49.72	5.07	-0.21	-0.22	10.19
	E7	45.86	51.96	35.92	59.79	48.90	5.19	-0.14	-0.46	10.62
	BLUP	43.52	52.92	39.23	59.51	48.47	4.13	0.12	-0.43	8.52
粒长/mm	E1	5.90	7.01	5.60	7.42	6.51	0.37	0.04	-0.11	5.64	0.91
Grain	E2	5.96	7.46	5.82	7.58	6.64	0.34	0.02	0.08	5.18
length	E3	6.37	7.31	5.69	7.72	6.72	0.37	-0.13	0.27	5.47
	E4	6.24	7.06	3.39	7.70	6.78	0.31	0.02	0.13	4.55
	E5	6.47	7.81	5.98	7.68	6.97	0.32	-0.19	0.00	4.59
	E6	6.76	7.81	6.09	7.90	7.12	0.34	-0.22	0.12	4.83
	E7	6.79	7.78	6.05	7.84	7.16	0.36	-0.28	0.12	4.69
	BLUP	6.38	7.43	5.96	7.58	6.84	0.30	-0.22	0.20	4.43
粒宽/mm	E1	2.94	3.19	2.88	3.74	3.24	0.18	0.18	0.28	5.48	0.87
Grain	E2	3.21	3.45	2.63	3.93	3.33	0.19	-0.30	1.02	5.78
width	E3	3.29	3.51	3.02	4.87	3.43	0.21	2.52	15.58	6.25
	E4	3.22	3.24	2.86	3.92	3.27	0.15	0.27	1.81	4.73
	E5	3.42	3.61	3.12	3.89	3.48	0.17	0.23	-0.28	4.75
	E6	3.39	3.46	3.01	4.27	3.43	0.16	0.72	4.81	4.79
	E7	3.41	3.44	3.03	4.08	3.42	0.16	0.14	1.76	4.62
	BLUP	3.28	3.41	3.07	3.92	3.37	0.13	0.54	2.07	3.75

性状 Trait	环境 Environments	亲本 Parents		DH群体(124份) DH population (124 copies)							遗传力 H²
性状 Trait	环境 Environments	安农 859 AN859	武农988 WN988	最小值 Min	最大值 Max	均值 Mean	标准差 s	偏度 Skew.	峰度 Kurtosis	变异系数/% CV	遗传力 H²
千粒质量/g	E1	34.01	44.25	31.18	61.17	45.58	5.78	-0.18	0.08	12.67	0.87
Thousand	E2	42.50	59.72	32.96	68.76	49.57	6.55	0.18	0.07	13.22
grains	E3	45.11	61.26	40.89	67.96	52.62	5.87	0.27	-0.55	11.15
weight	E4	37.94	42.47	30.83	57.42	42.46	4.89	-0.02	0.17	11.49
	E5	47.96	59.44	38.74	67.64	50.35	5.53	0.33	-0.17	10.98
	E6	47.99	54.34	36.93	62.19	49.72	5.07	-0.21	-0.22	10.19
	E7	45.86	51.96	35.92	59.79	48.90	5.19	-0.14	-0.46	10.62
	BLUP	43.52	52.92	39.23	59.51	48.47	4.13	0.12	-0.43	8.52
粒长/mm	E1	5.90	7.01	5.60	7.42	6.51	0.37	0.04	-0.11	5.64	0.91
Grain	E2	5.96	7.46	5.82	7.58	6.64	0.34	0.02	0.08	5.18
length	E3	6.37	7.31	5.69	7.72	6.72	0.37	-0.13	0.27	5.47
	E4	6.24	7.06	3.39	7.70	6.78	0.31	0.02	0.13	4.55
	E5	6.47	7.81	5.98	7.68	6.97	0.32	-0.19	0.00	4.59
	E6	6.76	7.81	6.09	7.90	7.12	0.34	-0.22	0.12	4.83
	E7	6.79	7.78	6.05	7.84	7.16	0.36	-0.28	0.12	4.69
	BLUP	6.38	7.43	5.96	7.58	6.84	0.30	-0.22	0.20	4.43
粒宽/mm	E1	2.94	3.19	2.88	3.74	3.24	0.18	0.18	0.28	5.48	0.87
Grain	E2	3.21	3.45	2.63	3.93	3.33	0.19	-0.30	1.02	5.78
width	E3	3.29	3.51	3.02	4.87	3.43	0.21	2.52	15.58	6.25
	E4	3.22	3.24	2.86	3.92	3.27	0.15	0.27	1.81	4.73
	E5	3.42	3.61	3.12	3.89	3.48	0.17	0.23	-0.28	4.75
	E6	3.39	3.46	3.01	4.27	3.43	0.16	0.72	4.81	4.79
	E7	3.41	3.44	3.03	4.08	3.42	0.16	0.14	1.76	4.62
	BLUP	3.28	3.41	3.07	3.92	3.37	0.13	0.54	2.07	3.75

性状 Traits	系数 Coefficient	标准误 Standard error	方差膨胀系数 VIF	拟合优度 R²
常量 Constant	-70.982	4.080		0.880
粒长 Grain length	4.900	0.445	1.069
粒宽 Grain width	25.508	1.067	1.069

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