Construction of a Genetic Linkage Map and QTL Mapping for Black Rot Resistance in Cabbage

doi:10.7668/hbnxb.20193907

Abstract

Abstract:

In order to further definite the genetic basis of black rot resistance in cabbage,and breed high-quality disease resistant cultivars,F₁ progenies were obtained by crossing the inbred line 4674(high resistance to race 1)as male parent,with the inbred line 4673(high susceptibility to race 1)as female parent.The F₂ population containing 152 individuals were obtained by F₁self-crossing. The F₂ population was inoculated by spraying at seedling stage. After 12-14 days, the phenotype of F₂ population was identified according to the identification method of cabbage seedling stage.A total of 175 markers with good polymorphism and clear bands were selected from 404 pairs.Subsequently, the 175 molecular markers were used for genotyping of F₂ population and constructing a genetic linkage map. Finally, the QTL for black rot resistance of cabbage was mapped with phenotypic data and genetic map.The results showed that there were 154 molecular markers linked to nine chromosomes,including 110 pairs of InDel markers and 44 pairs of SSR markers and covering a length of 714.29 cM,and the average distance between markers was 4.64 cM.Seven QTLs were located,of which three major QTLs were qBR-7-2,qBR-7-3 and qBR-4-3,mapped on the genomic markers(CG842110-CG842482 and M29-M39)of chromosome 7,and on the genomic markers between CD838151 and BOE417 of chromosome 4,respectively.The explainable phenotypic variation of QTLs were 16.0%,9.2% and 10.0%,and their LOD values were 5.75,3.20 and 3.47,respectively.

Key words: Cabbage, Black rot, F₂ group, Genetic linkage map, QTL mapping

YUE Xiangqing, ZHONG Xionghui, CUI Jian, HAN Rui, SONG Xumeng, XIE Jianming, KANG Jungen. Construction of a Genetic Linkage Map and QTL Mapping for Black Rot Resistance in Cabbage[J]. Acta Agriculturae Boreali-Sinica, 2023, 38(3): 167-175. doi: 10.7668/hbnxb.20193907.

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References 39

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染色体 Chromosome	长度/cM Length	标记数 Number of Markers	平均图距/cM Average distance	最大空隙/cM Biggest interval	最小空隙/cM Smallest interval	平均标记数/cM Average interval
CO1	65.21	17	3.84	12.2	0.1	0.26
CO2	66.71	10	6.67	11.3	2.4	0.15
CO3	72.90	13	5.61	14.4	2.8	0.18
CO4	100.03	13	7.69	16.0	1.7	0.13
CO5	129.47	16	8.09	19.3	2.1	0.12
CO6	67.62	15	4.51	10.9	1.0	0.22
CO7	83.82	33	2.54	12.5	0.1	0.39
CO8	61.24	17	3.60	9.5	1.0	0.28
CO9	67.31	20	3.37	6.5	0.9	0.30
共计	714.29	154	4.64	12.5		0.26

染色体 Chromosome	长度/cM Length	标记数 Number of Markers	平均图距/cM Average distance	最大空隙/cM Biggest interval	最小空隙/cM Smallest interval	平均标记数/cM Average interval
CO1	65.21	17	3.84	12.2	0.1	0.26
CO2	66.71	10	6.67	11.3	2.4	0.15
CO3	72.90	13	5.61	14.4	2.8	0.18
CO4	100.03	13	7.69	16.0	1.7	0.13
CO5	129.47	16	8.09	19.3	2.1	0.12
CO6	67.62	15	4.51	10.9	1.0	0.22
CO7	83.82	33	2.54	12.5	0.1	0.39
CO8	61.24	17	3.60	9.5	1.0	0.28
CO9	67.31	20	3.37	6.5	0.9	0.30
共计	714.29	154	4.64	12.5		0.26

亲本Parents		F₂群体F₂ population
母本4673 Female parent 4673	父本4674 Male parent 4674	中亲值 Median relative value	变幅 Ranee	标准差 Std Dev	平均值 Mean	偏度 Skewness	峰度 Kurtosis	方差 Variance
7	0	3.5	0~7	2.24	3.23	0.28	-0.98	4.98

亲本Parents		F₂群体F₂ population
母本4673 Female parent 4673	父本4674 Male parent 4674	中亲值 Median relative value	变幅 Ranee	标准差 Std Dev	平均值 Mean	偏度 Skewness	峰度 Kurtosis	方差 Variance
7	0	3.5	0~7	2.24	3.23	0.28	-0.98	4.98

QTL	位置/cM Position	连锁群 Linkage group	置信区间/cM Confidence interval	侧翼标记 Flanking markers	LOD值 LOD value	加性效应 Additive effect	遗传效应 Genetic effect	表型贡献率/% Phenotypic variation explained
qBR-4-1	17.7	4	17.7~27.1	8C0101~CD823181	1.93	0.83	正向	5.7
qBR-4-2	48.6	4	48.6~53.8	CD819660~CD728591	2.44	0.89	正向	7.1
qBR-4-3	65.4	4	65.4~77.4	CD838151~BOE417	3.47	0.92	正向	10.0
qBR-6-1	60.7	6	55.0~60.7	BOE569~BOE761	1.63	-0.54	负向	4.8
qBR-7-1	12.2	7	10.3~12.2	BOSF1395~BOSF2672	2.47	0.45	正向	7.2
qBR-7-2	34.3	7	34.0~34.3	CG842110~CG842482	5.75	0.12	正向	16.0
qBR-7-3	71.3	7	71.3~83.8	M29~M39	3.20	0.59	正向	9.2

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