Identification of QTL for Aluminum Tolerance Related Traits at the Early Seedling Stage in Sesame

doi:10.7668/hbnxb.20193137

Abstract

Abstract:

To elucidate the genetic mechanism and identify quantitative trait loci(QTL)for Aluminum(Al)tolerance in sesame.We investigated the response of Al stress using sesame landraces Jinhuangma(JHM)and Zhushanbai(ZSB)with different concentrations of Al³⁺ and confirmed a suitable Al³⁺ concentration for stress.Three traits including relative root length(RRL),relative shoot length(RSL)and relative fresh seedling weight(RSW)were evaluated in a recombinant inbred line(RIL)population with 180 lines that derived from two parents that contrasts for Al tolerance(ZSB and JHM).Quantitative trait loci(QTL)mapping were conducted by using a high resolution genetic map containing 1 354 bin markers through composition interval mapping(CIM)method.Significant positive correlations were observed among the three traits.A total of 7 QTLs were detected on 6 chromosomes(Chr2,Chr4,Chr6,Chr10,Chr11 and Chr13),including 3 RRL related QTLs,2 RSL related QTLs and 2 RSW associated QTLs.Among them,two QTLs associated with RRL and RSW were mapped to the overlapped interval on chromosome 2.Putative candidate gene analysis revealed that 15 genes may be related to Al tolerance.These genes mainly involved in metal transport and detoxification,transmembrane binding of metal ions,GDSL-motif lipase,peroxisomal catalase and metabolism and detoxification of toxic substances in organisms.

Key words: Sesame, Al stress, QTL mapping, Candidate gene

LIANG Junchao, ZHAO Yunyan, WANG Zhiqi, SUN Jian, YAN Tingxian, YAN Xiaowen, RAO Yueliang, ZHOU Hongying, LE Meiwang. Identification of QTL for Aluminum Tolerance Related Traits at the Early Seedling Stage in Sesame[J]. Acta Agriculturae Boreali-Sinica, 2022, 37(6): 124-131. doi: 10.7668/hbnxb.20193137.

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References 39

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性状 Traits	金黄麻 JHM	竹山白 ZSB	RIL群体平均值 RIL mean	RIL群体变幅 RIL range
相对根长Relative root length	0.58	0.19	0.57	0.21~1.01
相对芽长Relative shoot length	0.78	0.39	0.81	0.53~1.01
相对苗鲜质量Relative fresh seedling weight	0.78	0.41	0.77	0.47~1.01

性状 Traits	金黄麻 JHM	竹山白 ZSB	RIL群体平均值 RIL mean	RIL群体变幅 RIL range
相对根长Relative root length	0.58	0.19	0.57	0.21~1.01
相对芽长Relative shoot length	0.78	0.39	0.81	0.53~1.01
相对苗鲜质量Relative fresh seedling weight	0.78	0.41	0.77	0.47~1.01

性状 Traits	相对根长 RRL	相对芽长 RSL	相对苗鲜质量 RSW
相对根长RRL	1.000
相对芽长RSL	0.646^**	1.000
相对苗鲜质量RSW	0.517^**	0.532^**	1.000

性状 Traits	相对根长 RRL	相对芽长 RSL	相对苗鲜质量 RSW
相对根长RRL	1.000
相对芽长RSL	0.646^**	1.000
相对苗鲜质量RSW	0.517^**	0.532^**	1.000

性状 Traits	QTL	染色体 Chromosome	标记区间 Marker interval	LOD	加性效应 Additive effect	表型贡献率/% R²
相对根长	qRRL2	Chr2	c02b009~c02b013	3.24	0.05	7.01
RRL	qRRL10	Chr10	c10b018~c10b019	2.63	-0.05	5.92
	qRRL11	Chr11	c11b024~c11b025	2.55	0.04	5.46
相对芽长	qRSL6	Chr6	c06b062~c06b066	3.55	0.03	7.75
RSL	qRSL13	Chr13	c13b079~c13b080	3.12	0.02	6.68
相对苗鲜质量	qRSW2	Chr2	c02b008~c02b010	2.68	0.02	5.85
RSW	qRSW4	Chr4	c04b064~c04b065	2.55	-0.03	5.95

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