Difference in Nitrogen Absorption,Transportation and Utilization of Sesame Varieties with Contrasting Nitrogen Efficiency at Seedling Stage

doi:10.7668/hbnxb.20194322

Abstract

Abstract:

In order to explore the law of nitrogen absorption,transport and utilization of sesame at seedling stage,two sesame varieties with different nitrogen efficiency,including Zhengzhi HL05(ZZ,high efficiency)and Myanmar high yielder(MD,nitrogen inefficiency)were used as materials.The differences in root morphology,nitrogen absorption,transportation and utilization,nitrogen metabolism indices and related gene expressions involved were compared between the two accessions through hydroponics,under normal nitrogen(CK,17.86 mmol/L)and low nitrogen(LN,0.2 mmol/L)levels respectively.The results showed that under different nitrogen concentrations,the root morphological indexes,biomass,nitrogen accumulation,enzyme activities related to nitrogen metabolism and root nitrogen use efficiency of ZZ variety were higher than those of MD on the whole.Low nitrogen stress significantly decreased the biomass,nitrogen accumulation,and leaf nitrogen metabolism-related enzyme activities,increased root morphological indexes,root activity,root-shoot ratio,nitrogen physiological use efficiency,root dry matter use efficiency and nitrogen use efficiency.Among them,the biomass,nitrogen accumulation,transport coefficient,root nitrogen physiological use efficiency and nitrogen use efficiency of ZZ were 2.48,1.08,1.73,2.36 and 2.65 folds of MD,respectively.qRT-PCR results showed that under low nitrogen stress,SiNPF6.3a/b,SiNPF4.6a,SiNRT2.4a/b,SiNRT2.5,SiNPF7.3a/b and SiNPF2.13 genes involved in $NO 3 -$ absorption,transport and redistribution of the nitrogen efficient variety ZZ in leaves were up-regulated,and the gene expression levels were higher than those of the nitrogen inefficient variety MD.In Conclusion,under low nitrogen stress,nitrogen efficient sesame varieties had more developed roots and higher ability of nitrogen assimilation,transport and redistribution at seedling stage,so as to obtain higher nitrogen accumulation and nitrogen use efficiency.

Key words: Sesame, Seedling stage, Nitrogen efficiency, Absorption and transportation, Utilization

ZHANG Pengyu, WANG Dongyong, GAO Tongmei, SU Xiaoyu, LI Feng, DAI Jin, TIAN Yuan, FU Jinzhou. Difference in Nitrogen Absorption,Transportation and Utilization of Sesame Varieties with Contrasting Nitrogen Efficiency at Seedling Stage[J]. Acta Agriculturae Boreali-Sinica, 2023, 38(6): 134-143. doi: 10.7668/hbnxb.20194322.

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Figures/Tables 9

References 42

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基因名称 Gene name	基因ID Gene ID	引物序列(5'—3') Primer sequence(5'—3')
基因名称 Gene name	基因ID Gene ID	前引物Forward primer	后引物Reverse primer
SiNRT2.5	LOC105156629	CTACCTATCATCCGAGACAACC	GTGCAGTGAGTAAAGTGAGAGA
SiNRT2.1	LOC105170268	AGACGATCGTTGGGCATAATAT	TGCATCCCACTATCATTATCCC
SiNRT2.4a	LOC105170269	GATGAGCACAATGTTCAACAGT	TTCATAAAGCACCGGCATTATC
SiNRT2.4b	LOC105170739	ATCTCGTTTTTCACCTGTTTCG	TTGGTGAGGTTCAGATTATCCC
SiNPF6.3a	LOC105155830	AATGGGGTTATGGAATTTGTGC	CACGAGTTTCTTGAAACGGTAG
SiNPF6.3b	LOC105164296	GAATAGCGGTGAATCTTGTCAC	AGTGCCAAGAAAATTCGTAACC
SiNPF4.6a	LOC105156563	AATACTCACAATACAAGCACGC	GTGCTCTCATCAAACTGTTCAG
SiNPF2.7a	LOC105170342	CATTTTTCTCATAGACCGGCTG	CCAAACGTGCTATTTTTAGCCT
SiNPF2.7b	LOC105170865	GGCAGGCAAGAACCCTACAT	CACTTGCCCCGCAAAATGAA
SiNPF7.3a	LOC105155943	TATGCTCAGAAAGATTGCAAGC	TAGACACCAGCAAGCTACTAAC
SiNPF7.3b	LOC105167169	GTGTCGATTTACCTAATTGCCC	CTTGAAATGCCTATACGTTGGG
SiNPF2.13	LOC105168154	GGTTTTGATCATTGCACTGACT	ATACACATACAATCTCGTCCCG

基因名称 Gene name	基因ID Gene ID	引物序列(5'—3') Primer sequence(5'—3')
基因名称 Gene name	基因ID Gene ID	前引物Forward primer	后引物Reverse primer
SiNRT2.5	LOC105156629	CTACCTATCATCCGAGACAACC	GTGCAGTGAGTAAAGTGAGAGA
SiNRT2.1	LOC105170268	AGACGATCGTTGGGCATAATAT	TGCATCCCACTATCATTATCCC
SiNRT2.4a	LOC105170269	GATGAGCACAATGTTCAACAGT	TTCATAAAGCACCGGCATTATC
SiNRT2.4b	LOC105170739	ATCTCGTTTTTCACCTGTTTCG	TTGGTGAGGTTCAGATTATCCC
SiNPF6.3a	LOC105155830	AATGGGGTTATGGAATTTGTGC	CACGAGTTTCTTGAAACGGTAG
SiNPF6.3b	LOC105164296	GAATAGCGGTGAATCTTGTCAC	AGTGCCAAGAAAATTCGTAACC
SiNPF4.6a	LOC105156563	AATACTCACAATACAAGCACGC	GTGCTCTCATCAAACTGTTCAG
SiNPF2.7a	LOC105170342	CATTTTTCTCATAGACCGGCTG	CCAAACGTGCTATTTTTAGCCT
SiNPF2.7b	LOC105170865	GGCAGGCAAGAACCCTACAT	CACTTGCCCCGCAAAATGAA
SiNPF7.3a	LOC105155943	TATGCTCAGAAAGATTGCAAGC	TAGACACCAGCAAGCTACTAAC
SiNPF7.3b	LOC105167169	GTGTCGATTTACCTAATTGCCC	CTTGAAATGCCTATACGTTGGG
SiNPF2.13	LOC105168154	GGTTTTGATCATTGCACTGACT	ATACACATACAATCTCGTCCCG

品种 Variety	处理 Treatment	生物量/(g/株) Biomass	地上部干质量/(g/株) Shoot dry weight	根干质量/(g/株) Root dry weight	根冠比 Root-shoot ratio
ZZ	CK	1.563±0.008a	1.318±0.008a	0.245±0.001a	0.186±0.002c
	LN	0.452±0.006c	0.358±0.007c	0.094±0.001b	0.263±0.001a
	LN/CK	0.29	0.27	0.38	1.41
MD	CK	0.697±0.022b	0.603±0.019b	0.064±0.001b	0.106±0.007c
	LN	0.182±0.009d	0.147±0.007d	0.035±0.003c	0.238±0.012b
	LN/CK	0.26	0.24	0.37	2.25

品种 Variety	处理 Treatment	生物量/(g/株) Biomass	地上部干质量/(g/株) Shoot dry weight	根干质量/(g/株) Root dry weight	根冠比 Root-shoot ratio
ZZ	CK	1.563±0.008a	1.318±0.008a	0.245±0.001a	0.186±0.002c
	LN	0.452±0.006c	0.358±0.007c	0.094±0.001b	0.263±0.001a
	LN/CK	0.29	0.27	0.38	1.41
MD	CK	0.697±0.022b	0.603±0.019b	0.064±0.001b	0.106±0.007c
	LN	0.182±0.009d	0.147±0.007d	0.035±0.003c	0.238±0.012b
	LN/CK	0.26	0.24	0.37	2.25

品种 Variety	处理 Treatment	总根长/cm Total root length	根表面积/cm² Root surface area	根体积/cm³ Root volume	根直径/mm Root diameter	侧根数/条 Lateral root number	根系活力/ (μg/(g·h)) Root activity
ZZ	CK	660.41±55.87c	66.95±6.33c	0.60±0.06c	0.32±0.00b	752.00±71.59c	1 156.39±40.71c
	LN	2 269.93±142.42a	275.43±14.32a	2.67±0.06a	0.38±0.02a	2 338.00±68.79a	3 211.92±127.86a
	LN/CK	3.44	4.11	4.45	1.19	3.11	2.78
MD	CK	533.23±28.51d	52.99±3.16d	0.40±0.03d	0.32±0.02b	459.00±12.17d	944.63±51.72d
	LN	1 771.35±116.36b	91.26±28.35b	1.54±0.28b	0.32±0.00b	1 797.67±67.04b	1 696.56±61.32b
	LN/CK	3.32	1.72	3.85	1.00	3.92	1.80

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