QTL Mapping of Silk Coloring Characteristics of Anthocyanins in Maize

doi:10.7668/hbnxb.20194658

Abstract

Abstract:

Silk color is an important agronomic trait for determining distinctiveness and uniformity of maize variety.In order to analyze the genetic mechanism of silk coloring characteristics of anthocyanins in maize,a doubled haploid(DH)population with 213 lines derived from green silk inbred line WL134 and purple silk inbred line D7 was used for QTL mapping analysis under the environment of 2022 and 2023,respectively.The results showed that there were significant differences in silk coloring characteristics of anthocyanins among different lines and years,the heritability was 0.864.A total of 9 QTLs were detected in two years.These QTLs with phenotypic variation explained(PVE)ranging from 4.83% to 9.26% were detected on chromosomes 2,3,5,6,8,and 9 of maize.A stable and repeatable site qSC5 located between 19.15 Mb and 19.80 Mb was detected on chromosome 5 in two-year data.The LOD scores of the major QTLs were 4.65 and 5.76 in 2022 and 2023 respectively,with phenotypic variation explained(PVE)of 7.22% and 7.17%.It was a new site for regulating silk coloring characteristics of anthocyanins compared with previous studies in maize.Based on SNP markers on both sides of qSC5,90.91% of the genotypes in extreme purple silk DH lines were CCCC,while only 44.00% of the genotypes in green silk DH lines.This marker was significantly correlated with silk color in maize and might link to key genes regulating silk color characteristics of anthocyanins.

Key words: Maize, Silk color, QTL mapping, SNP marker

HUANG Huanhuan, AN Hongzhou, LI Kuiying, WANG Yanbing, GU Yi, QIAO Yake, GAO Zengyu. QTL Mapping of Silk Coloring Characteristics of Anthocyanins in Maize[J]. Acta Agriculturae Boreali-Sinica, 2024, 39(4): 31-36. doi: 10.7668/hbnxb.20194658.

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References 26

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差异源 Source of variance	平方和 SS	自由度 df	均方 MS	F值 F-value	P值 P-value	显著性 Significance	遗传力 H²
基因型(G)	623.600	212	2.942	10.426	3.53E-90	^**
年份(Y)	29.719	1	29.719	105.333	3.36E-22	^**
基因型×年份(G×Y)	73.043	208	0.351	1.245	0.032	^*
误差(E)	118.500	420	0.282
总计(T)	847.122	841					0.864

差异源 Source of variance	平方和 SS	自由度 df	均方 MS	F值 F-value	P值 P-value	显著性 Significance	遗传力 H²
基因型(G)	623.600	212	2.942	10.426	3.53E-90	^**
年份(Y)	29.719	1	29.719	105.333	3.36E-22	^**
基因型×年份(G×Y)	73.043	208	0.351	1.245	0.032	^*
误差(E)	118.500	420	0.282
总计(T)	847.122	841					0.864

年份 Year	QTL名称 QTL name	染色体 Chr	位置/Mb Pos	标记区间(左)/bp Left marker	标记区间(右)/bp Right marker	LOD值 LOD	贡献率/% PVE	加性效应 Add
2022	qSC2-1	2	0	chr2-8786937	chr2-8786986	5.88	9.26	0.258 2
2022	qSC5	5	58	chr5-19156257	chr5-19800985	4.65	7.22	0.199 3
2022	qSC6-1	6	81	chr6-141474871	chr6-142274037	3.39	5.35	0.173 2
2022	qSC8	8	86	chr8-140614324	chr8-142202388	4.23	6.63	0.190 4
2022	qSC9	9	108	chr9-142103909	chr9-142308333	4.05	6.31	-0.185 7
2023	qSC2-2	2	23	chr2-8955652	chr2-9007579	4.54	5.59	0.214 7
2023	qSC2-3	2	182	chr2-231587850	chr2-231194135	4.78	5.89	0.221 5
2023	qSC3	3	150	chr3-218266161	chr3-218273050	4.46	5.60	-0.213 0
2023	qSC5	5	58	chr5-19156257	chr5-19800985	5.76	7.17	0.244 4
2023	qSC6-2	6	95	chr6-149567395	chr6-150880011	3.96	4.83	0.204 3

年份 Year	QTL名称 QTL name	染色体 Chr	位置/Mb Pos	标记区间(左)/bp Left marker	标记区间(右)/bp Right marker	LOD值 LOD	贡献率/% PVE	加性效应 Add
2022	qSC2-1	2	0	chr2-8786937	chr2-8786986	5.88	9.26	0.258 2
2022	qSC5	5	58	chr5-19156257	chr5-19800985	4.65	7.22	0.199 3
2022	qSC6-1	6	81	chr6-141474871	chr6-142274037	3.39	5.35	0.173 2
2022	qSC8	8	86	chr8-140614324	chr8-142202388	4.23	6.63	0.190 4
2022	qSC9	9	108	chr9-142103909	chr9-142308333	4.05	6.31	-0.185 7
2023	qSC2-2	2	23	chr2-8955652	chr2-9007579	4.54	5.59	0.214 7
2023	qSC2-3	2	182	chr2-231587850	chr2-231194135	4.78	5.89	0.221 5
2023	qSC3	3	150	chr3-218266161	chr3-218273050	4.46	5.60	-0.213 0
2023	qSC5	5	58	chr5-19156257	chr5-19800985	5.76	7.17	0.244 4
2023	qSC6-2	6	95	chr6-149567395	chr6-150880011	3.96	4.83	0.204 3

极端紫花丝家系 Extreme purple silk lines	基因型 Genotype	花丝颜色分级 Silk color grading		极端绿花丝家系 Extreme green silk lines	基因型 Genotype	花丝颜色分级 Silk color grading
极端紫花丝家系 Extreme purple silk lines	基因型 Genotype	2022	2023	极端绿花丝家系 Extreme green silk lines	基因型 Genotype	2022	2023
L46	CCCC	4	6	L20	TTAA	1	1
L116	CCCC	5	4	L34	TTAA	1	1
L194	CCCC	4	4	L95	TTAA	1	1

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