Identification of QTLs for Length and Area of Three Leaves Near the Ear in Maize

doi:10.7668/hbnxb.20193135

Abstract

Abstract:

Leaf length and leaf area are two important agronomic traits,in order to study the genetic mechanism of leaf length and area of three leaves near the ear in maize,a recombinant inbred line(RIL)population(241 lines)derived from Zheng 58 and D863F was used for QTL mapping analysis under spring sowing and summer sowing in 2018 and 2019,respectively.In RILs population, leaf length and area of three leaves near the ear showed continuous variations with a normal distribution and belonged to typical quantitative trait, and significant correlation was found among the traits, the heritability of the six traits was 88.04%, 88.45%, 87.86%, 85.04%, 85.27% and 85.73%, respectively.In total,23 QTLs were detected in two years.The number of QTLs for leaf length and leaf area were 14 and 9,respectively.These QTLs with phenotypic variance explained(PVE)ranging from 5.84% to 17.81% were detected on chromosome 1,2,4,5,6 and 8.Among them,only three QTLs(qFirLL1-2,qFirLL5-1 and qSecLL1-2)for leaf length and two QTLs(qFirLA2-1 and qSecLA2-1)for leaf area were identified in both environments(spring and summer sowing),the remaining QTLs can only be detected separately in spring sowing or summer sowing environments.The major QTLs controlling six traits were detected on chromosome 2(bnlg1316—bnlg1141)and 5(umc1591—umc2298),which explained phenotypic variation ranging from 6.42% to 17.81%;These two marker intervals are important for regulating the length and area of three leaves near the ear, and might contain key genes regulating leaf traits.

Key words: Maize, Three leaves near the ear, Leaf length, Leaf area, QTL

WANG Xintao, YANG Qing, LI Baoye, DAI Ziju, HAO Junjie. Identification of QTLs for Length and Area of Three Leaves Near the Ear in Maize[J]. Acta Agriculturae Boreali-Sinica, 2022, 37(6): 115-123. doi: 10.7668/hbnxb.20193135.

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Figures/Tables 8

References 23

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环境 Environment	性状 Trait	亲本 Parent		RIL群体 RIL population
环境 Environment	性状 Trait	郑58 Zheng 58	D863F	均值±标准差 Mean±s	变幅 Range	峰度 Kurtosis	偏度 Skewness
2018春播	穗下叶叶长/cm	69.00±2.16	61.33±2.05	70.44±8.75	45.50~90.00	-0.12	-0.18
Spring sowing in 2018	穗位叶叶长/cm	63.67±3.09	62.00±1.63	67.56±8.67	42.00~88.50	-0.01	-0.22
	穗上叶叶长/cm	58.67±3.30	61.67±6.60	63.03±8.79	38.00~82.50	-0.44	-0.12
	穗下叶叶面积/cm²	353.63±22.90	375.67±30.62	389.22±82.33	204.31~704.69	0.09	0.07
	穗位叶叶面积/cm²	319.93±20.37	410.75±32.07	374.95±80.59	191.97~637.88	-0.21	0.01
	穗上叶叶面积/cm²	278.67±24.48	374.63±10.39	343.32±81.43	155.01~564.47	-0.53	0.02
2018夏播	穗下叶叶长/cm	78.03±1.29	77.28±2.72	77.23±8.57	50.20~97.75	-0.19	-0.02
Summer sowing in 2018	穗位叶叶长/cm	75.23±2.70	75.00±1.35	75.51±8.45	48.73~97.48	-0.12	0.12
	穗上叶叶长/cm	71.23±3.26	71.15±2.30	72.59±8.32	39.45~94.70	0.81	0.12
	穗下叶叶面积/cm²	421.34±15.76	547.69±12.56	444.93±70.92	221.23~718.62	0.89	0.17
	穗位叶叶面积/cm²	424.55±21.87	566.72±20.99	434.56±68.03	249.53~647.41	0.29	0.24
	穗上叶叶面积/cm²	363.25±18.11	504.28±12.86	410.95±68.15	196.02~627.06	0.31	0.22
2019春播	穗下叶叶长/cm	63.67±4.11	62.33±4.11	70.39±8.85	43.17~93.00	-0.03	-0.11
Spring sowing in 2019	穗位叶叶长/cm	62.47±5.65	62.00±2.45	67.25±8.91	36.67~92.17	0.23	-0.12
	穗上叶叶长/cm	58.77±2.39	57.00±2.94	62.98±8.85	33.33~84.00	-0.05	-0.25
	穗下叶叶面积/cm²	296.05±35.58	375.50±54.75	381.04±75.88	220.50~612.35	-0.18	0.01
	穗位叶叶面积/cm²	292.03±42.15	397.13±35.97	366.04±77.47	176.35~636.43	0.05	0.23
	穗上叶叶面积/cm²	267.39±5.28	358.65±37.48	337.64±77.83	152.18~573.62	-0.27	-0.01
2019夏播	穗下叶叶长/cm	74.13±1.47	71.08±2.63	79.39±8.48	55.45~104.63	0.23	0.00
Summer sowing in 2019	穗位叶叶长/cm	72.00±1.41	69.40±2.38	76.68±8.36	50.40~96.45	0.18	-0.19
	穗上叶叶长/cm	68.48±1.00	65.45±2.91	72.62±8.10	45.58~91.93	0.08	-0.32
	穗下叶叶面积/cm²	383.60±10.14	506.41±30.17	480.58±73.63	325.68~706.48	-0.06	0.38
	穗位叶叶面积/cm²	361.80±11.70	507.49±22.00	468.11±70.54	308.88~680.85	0.22	0.41
	穗上叶叶面积/cm²	353.07±14.20	458.97±31.19	432.61±67.81	274.23~610.31	-0.16	0.30

环境 Environment	性状 Trait	亲本 Parent		RIL群体 RIL population
环境 Environment	性状 Trait	郑58 Zheng 58	D863F	均值±标准差 Mean±s	变幅 Range	峰度 Kurtosis	偏度 Skewness
2018春播	穗下叶叶长/cm	69.00±2.16	61.33±2.05	70.44±8.75	45.50~90.00	-0.12	-0.18
Spring sowing in 2018	穗位叶叶长/cm	63.67±3.09	62.00±1.63	67.56±8.67	42.00~88.50	-0.01	-0.22
	穗上叶叶长/cm	58.67±3.30	61.67±6.60	63.03±8.79	38.00~82.50	-0.44	-0.12
	穗下叶叶面积/cm²	353.63±22.90	375.67±30.62	389.22±82.33	204.31~704.69	0.09	0.07
	穗位叶叶面积/cm²	319.93±20.37	410.75±32.07	374.95±80.59	191.97~637.88	-0.21	0.01
	穗上叶叶面积/cm²	278.67±24.48	374.63±10.39	343.32±81.43	155.01~564.47	-0.53	0.02
2018夏播	穗下叶叶长/cm	78.03±1.29	77.28±2.72	77.23±8.57	50.20~97.75	-0.19	-0.02
Summer sowing in 2018	穗位叶叶长/cm	75.23±2.70	75.00±1.35	75.51±8.45	48.73~97.48	-0.12	0.12
	穗上叶叶长/cm	71.23±3.26	71.15±2.30	72.59±8.32	39.45~94.70	0.81	0.12
	穗下叶叶面积/cm²	421.34±15.76	547.69±12.56	444.93±70.92	221.23~718.62	0.89	0.17
	穗位叶叶面积/cm²	424.55±21.87	566.72±20.99	434.56±68.03	249.53~647.41	0.29	0.24
	穗上叶叶面积/cm²	363.25±18.11	504.28±12.86	410.95±68.15	196.02~627.06	0.31	0.22
2019春播	穗下叶叶长/cm	63.67±4.11	62.33±4.11	70.39±8.85	43.17~93.00	-0.03	-0.11
Spring sowing in 2019	穗位叶叶长/cm	62.47±5.65	62.00±2.45	67.25±8.91	36.67~92.17	0.23	-0.12
	穗上叶叶长/cm	58.77±2.39	57.00±2.94	62.98±8.85	33.33~84.00	-0.05	-0.25
	穗下叶叶面积/cm²	296.05±35.58	375.50±54.75	381.04±75.88	220.50~612.35	-0.18	0.01
	穗位叶叶面积/cm²	292.03±42.15	397.13±35.97	366.04±77.47	176.35~636.43	0.05	0.23
	穗上叶叶面积/cm²	267.39±5.28	358.65±37.48	337.64±77.83	152.18~573.62	-0.27	-0.01
2019夏播	穗下叶叶长/cm	74.13±1.47	71.08±2.63	79.39±8.48	55.45~104.63	0.23	0.00
Summer sowing in 2019	穗位叶叶长/cm	72.00±1.41	69.40±2.38	76.68±8.36	50.40~96.45	0.18	-0.19
	穗上叶叶长/cm	68.48±1.00	65.45±2.91	72.62±8.10	45.58~91.93	0.08	-0.32
	穗下叶叶面积/cm²	383.60±10.14	506.41±30.17	480.58±73.63	325.68~706.48	-0.06	0.38
	穗位叶叶面积/cm²	361.80±11.70	507.49±22.00	468.11±70.54	308.88~680.85	0.22	0.41
	穗上叶叶面积/cm²	353.07±14.20	458.97±31.19	432.61±67.81	274.23~610.31	-0.16	0.30

环境 Environment	性状 Trait	穗下叶叶长 FirLL	穗位叶叶长 SecLL	穗上叶叶长 ThiLL	穗下叶叶面积 FirLA	穗位叶叶面积 SecLA	穗上叶叶面积 ThiLA
2018春播	穗下叶叶长	1.00
Spring sowing in 2018	穗位叶叶长	0.92^**	1.00
	穗上叶叶长	0.85^**	0.90^**	1.00
	穗下叶叶面积	0.79^**	0.73^**	0.69^**	1.00
	穗位叶叶面积	0.76^**	0.80^**	0.75^**	0.93^**	1.00
	穗上叶叶面积	0.71^**	0.73^**	0.82^**	0.86^**	0.92^**	1.00
2018夏播	穗下叶叶长	1.00
Summer sowing in 2018	穗位叶叶长	0.95^**	1.00
	穗上叶叶长	0.90^**	0.94^**	1.00
	穗下叶叶面积	0.73^**	0.69^**	0.63^**	1.00
	穗位叶叶面积	0.73^**	0.76^**	0.69^**	0.94^**	1.00
	穗上叶叶面积	0.70^**	0.73^**	0.75^**	0.88^**	0.92^**	1.00
2019春播	穗下叶叶长	1.00
Spring sowing in 2019	穗位叶叶长	0.91^**	1.00
	穗上叶叶长	0.84^**	0.90^**	1.00
	穗下叶叶面积	0.77^**	0.70^**	0.70^**	1.00
	穗位叶叶面积	0.75^**	0.79^**	0.74^**	0.91^**	1.00
	穗上叶叶面积	0.67^**	0.71^**	0.83^**	0.86^**	0.88^**	1.00
2019夏播	穗下叶叶长	1.00
Summer sowing in 2019	穗位叶叶长	0.94^**	1.00
	穗上叶叶长	0.85^**	0.94^**	1.00
	穗下叶叶面积	0.68^**	0.58^**	0.47^**	1.00
	穗位叶叶面积	0.69^**	0.69^**	0.62^**	0.91^**	1.00
	穗上叶叶面积	0.66^**	0.69^**	0.71^**	0.82^**	0.92^**	1.00

环境 Environment	性状 Trait	穗下叶叶长 FirLL	穗位叶叶长 SecLL	穗上叶叶长 ThiLL	穗下叶叶面积 FirLA	穗位叶叶面积 SecLA	穗上叶叶面积 ThiLA
2018春播	穗下叶叶长	1.00
Spring sowing in 2018	穗位叶叶长	0.92^**	1.00
	穗上叶叶长	0.85^**	0.90^**	1.00
	穗下叶叶面积	0.79^**	0.73^**	0.69^**	1.00
	穗位叶叶面积	0.76^**	0.80^**	0.75^**	0.93^**	1.00
	穗上叶叶面积	0.71^**	0.73^**	0.82^**	0.86^**	0.92^**	1.00
2018夏播	穗下叶叶长	1.00
Summer sowing in 2018	穗位叶叶长	0.95^**	1.00
	穗上叶叶长	0.90^**	0.94^**	1.00
	穗下叶叶面积	0.73^**	0.69^**	0.63^**	1.00
	穗位叶叶面积	0.73^**	0.76^**	0.69^**	0.94^**	1.00
	穗上叶叶面积	0.70^**	0.73^**	0.75^**	0.88^**	0.92^**	1.00
2019春播	穗下叶叶长	1.00
Spring sowing in 2019	穗位叶叶长	0.91^**	1.00
	穗上叶叶长	0.84^**	0.90^**	1.00
	穗下叶叶面积	0.77^**	0.70^**	0.70^**	1.00
	穗位叶叶面积	0.75^**	0.79^**	0.74^**	0.91^**	1.00
	穗上叶叶面积	0.67^**	0.71^**	0.83^**	0.86^**	0.88^**	1.00
2019夏播	穗下叶叶长	1.00
Summer sowing in 2019	穗位叶叶长	0.94^**	1.00
	穗上叶叶长	0.85^**	0.94^**	1.00
	穗下叶叶面积	0.68^**	0.58^**	0.47^**	1.00
	穗位叶叶面积	0.69^**	0.69^**	0.62^**	0.91^**	1.00
	穗上叶叶面积	0.66^**	0.69^**	0.71^**	0.82^**	0.92^**	1.00

性状 Trait	变异来源 Variation source	平方和 Sum of square	均方 Mean square	F	遗传力 h²
穗下叶叶长	基因型	136 435.17	649.69	30.46^**	88.04
FirLL	环境	38 186.47	12 728.82	596.76^**
	基因型×环境	53 584.20	85.33	4.00^**
穗位叶叶长	基因型	138 195.52	658.07	29.71^**	88.45
SecLL	环境	46 328.04	15 442.68	697.27^**
	基因型×环境	52 132.66	83.01	3.75^**
穗上叶叶长	基因型	133 005.27	633.36	27.26^**	87.86
ThiLL	环境	56 785.32	18 928.44	814.63^**
	基因型×环境	52 949.49	84.31	3.63^**
穗下叶叶面积	基因型	9 709 479.00	46 235.61	25.18^**	85.04
FirLA	环境	4 009 453.00	1 336 484.38	727.96^**
	基因型×环境	4 905 605.00	7 811.47	4.25^**
穗位叶叶面积	基因型	9 562 553.00	45 535.96	25.16^**	85.27
SecLA	环境	4 370 409.00	1 456 803.00	805.06^**
	基因型×环境	4 743 622.00	7 553.54	4.17^**
穗上叶叶面积	基因型	9 535 236.00	45 405.89	25.27^**	85.73
ThiLA	环境	4 374 459.50	1 458 153.13	811.40^**
	基因型×环境	4 558 320.00	7 258.47	4.04^**

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