Cytological Observation and SSR Analysis of New Colored Potato Strains

doi:10.7668/hbnxb.20193007

Abstract

Abstract:

In order to clarify the genetic differences of the six new-colored potato strains NNCS-1,NNCS-5,NNCS-6,NNCS-7,NNCS-33 and NNCS-37 at the cytogenetic and DNA molecular level,this experiment used material MIN-021 as control,and the regular staining and slides preparing and SSR marker technology were used to analyze the pollen fertility,chromosome configuration at meiosis metaphase Ⅰ of pollen mother cells and SSR polymorphism of the above six varieties.The results shaved that the pollen fertility of the six new-colored potato strains were 44.00%,66.78%,74.44%,60.70%,78.10% and 80.22%,respectively.And the chromosome pairing configurations of these test materials were 8.45Ⅰ+6.09Ⅱ+7.35Ⅲ+1.33Ⅳ,6.91Ⅰ+7.13Ⅱ+5.73Ⅲ+2.41Ⅳ,5.19Ⅰ+9.16Ⅱ+4.51Ⅲ+2.74Ⅳ,6.55Ⅰ+8.35Ⅱ+4.97Ⅲ+2.46Ⅳ,4.02Ⅰ+10.15Ⅱ+3.40Ⅲ+3.37Ⅳ and 3.31Ⅰ+10.42Ⅱ+2.99Ⅲ+3.72Ⅳ,respectively,indicating that there were certain differences among the strains at the cytological level.Ten suitable SSR primers were selected to amplify the genomic DNA of each new strain and control material,and 151 SSR polymorphic sites were obtained,accounting for 86.78% of the total number of polymorphic loci.The genetic differences among the materials were revealed.In addition,a SSR fingerprint was amplified with the selected specific primers C42,which could identify six new-colored potato strains and control.Based on the average genetic distance(GD=0.62),the seven materials were divided into three categories: the new strain NNCS-37,NNCS-6,NNCS-7,NNCS-5 and the control(MIN-021)belong to one categorie.The new strains NNCS-33 and NNCS-1 were separately classified as a categorie.

Key words: New colored potato strain, Cytogenetics, SSR analysis

YUE Dong, YU Zhuo, YU Xiaoxia, LI Jiaqi, LI Jingwei, YANG Dongsheng, ZHANG Hailong, LI Zhicong. Cytological Observation and SSR Analysis of New Colored Potato Strains[J]. Acta Agriculturae Boreali-Sinica, 2022, 37(6): 108-114. doi: 10.7668/hbnxb.20193007.

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References 25

[1]	宗洪霞, 徐茜, 李保证, 肖波. 彩色马铃薯新品种(系)引进与筛选研究[J]. 湖北农业科学, 2021, 60(13): 36-38,59.doi:10.14088/j.cnki.issn0439-8114.2021.13.007. doi: 10.14088/j.cnki.issn0439-8114.2021.13.007
	Zong H X, Xu Q, Li B Z, Xiao B. Study on the introduction and screening of new colored potato varieties(lines)[J]. Hubei Agricultural Sciences, 2021, 60(13): 36-38,59.
[2]	郭凤丹, 王效忠, 刘学英, 夏晗, 王兴军. 植物花青素生物代谢调控[J]. 生命科学, 2011, 23(10): 938-944.doi:10.13376/j.cbls/2011.10.011. doi: 10.13376/j.cbls/2011.10.011
	Guo F D, Wang X Z, Liu X Y, Xia H, Wang X J. Metabolic regulation of plants anthocyanin[J]. Chinese Bulletin of Life Sciences, 2011, 23(10): 938-944.
[3]	Han K H, Shimada K I, Sekikawa M, Fukushima M. Anthocyanin-rich red potato flakes affect serum lipid peroxidation and hepatic SOD mRNA level in rats[J]. Bioscience,Biotechnology,and Biochemistry, 2007, 71(5): 1356-1359.doi:10.1271/bbb.70060. doi: 10.1271/bbb.70060
[4]	仇菊, 刘鹏, 孙君茂. 彩色马铃薯营养保健功能及其食品开发研究进展[J]. 食品与机械, 2016, 32(10): 226-229.doi:10.13652/j.issn.1003-5788.2016.10.049. doi: 10.13652/j.issn.1003-5788.2016.10.049
	Qiu J, Liu P, Sun J M. Research advances in health functions and food development of colored potatoes[J]. Food & Machinery, 2016, 32(10): 226-229.
[5]	Brown C. Nutrient status of potato:Assessment of future trends[C]. Washington: Proceeding of the Washington State Popato Conference, 2004, 43(5):11-17.
[6]	罗文彬, 李华伟, 许国春, 许泳清, 纪荣昌, 邱思鑫, 汤浩. 彩色马铃薯种质资源的引进与评价[J]. 福建农业学报, 2019, 34(3): 278-283.doi:10.19303/j.issn.1008-0384.2019.03.004. doi: 10.19303/j.issn.1008-0384.2019.03.004
	Luo W B, Li H W, Xu G C, Xu Y Q, Ji R C, Qiu S X, Tang H. Introduction and evaluation of colored potato germplasms[J]. Fujian Journal of Agricultural Sciences, 2019, 34(3): 278-283.
[7]	原霁虹, 赵菊梅, 陈亚兰, 高娜, 陈鑫. 甘肃省定西地区引种彩色马铃薯性状比较及产量评价[J]. 中国食物与营养, 2019, 25(9): 14-16.doi:10.19870/j.cnki.11-3716/ts.2019.09.003. doi: 10.19870/j.cnki.11-3716/ts.2019.09.003
	Yuan J H, Zhao J M, Chen Y L, Gao N, Chen X. Trait comparison and yield evaluation of color potato introduced into Dingxi of Gansu Province[J]. Food and Nutrition in China, 2019, 25(9): 14-16.
[8]	温海霞, 汤三明, 宿秀丽, 吴国文, 朱杰, 方治国, 陈斐, 赵红, 冯鹏. 不同马铃薯品种品质筛选分析[J]. 湖北农业科学, 2021, 60(10): 21-25.doi:10.14088/j.cnki.issn0439-8114.2021.10.005. doi: 10.14088/j.cnki.issn0439-8114.2021.10.005
	Wen H X, Tang S M, Su X L, Wu G W, Zhu J, Fang Z G, Chen F, Zhao H, Feng P. Quality screening analysis of different potato varieties[J]. Hubei Agricultural Sciences, 2021, 60(10): 21-25.
[9]	李飞, 李标, 孙伟, 邓宽平, 邓禄军, 雷尊国. 马铃薯亲本的花粉活力研究初报[J]. 贵州农业科学, 2009, 37(12): 47-48.doi: 10.3969/j.issn.1001-3601.2009.12.014. doi: 10.3969/j.issn.1001-3601.2009.12.014
	Li F, Li B, Sun W, Deng K P, Deng L J, Lei Z G. Preliminary report of pollen activity of potato parents[J]. Guizhou Agricultural Sciences, 2009, 37(12): 47-48.
[10]	Pérez-Figueroa A, Rodríguez-Ramilo S T, Caballero A. Analysis and management of gene and allelic diversity in subdivided populations using the software program METAPOP[M]//Data Production and Analysis in Population Genomics. Totowa, NJ: Humana Press, 2012:261-275.doi:10.1007/978-1-61779-870-2_15. doi: 10.1007/978-1-61779-870-2_15
[11]	安尼卡尔·艾斯卡尔, 祖来克孜·米吉提. 系统聚类法及其应用研究[J]. 价值工程, 2019, 38(17): 254-258.doi:10.14018/j.cnki.cn13-1085/n.2019.17.091. doi: 10.14018/j.cnki.cn13-1085/n.2019.17.091
	Annikaer A S K E, Zulaikezi M J T. Systematic clustering method and its application[J]. Value Engineering, 2019, 38(17): 254-258.
[12]	徐雁飞, 陈发棣, 陈素梅, 滕年军, 李风童. 5个菊花品种自交结实特性及减数分裂行为与花粉育性研究[J]. 西北植物学报, 2009, 29(3): 469-474.doi:10.3321/j.issn:1000-4025.2009.03.007. doi: 10.3321/j.issn:1000-4025.2009.03.007
	Xu Y F, Chen F D, Chen S M, Teng N J, Li F T. Seed set of inbred,meiosis and pollen capacity of 5 Chrysanthemum cultivars[J]. Acta Botanica Boreali-Occidentalia Sinica, 2009, 29(3): 469-474.
[13]	朴锦, 具红光, 朴钟云. 关苍术花粉母细胞减数分裂及其雄配子体发育研究[J]. 西北植物学报, 2015, 35(8): 1547-1553.doi:10.7606/j.issn.1000-4025.2015.08.1547. doi: 10.7606/j.issn.1000-4025.2015.08.1547
	Piao J, Ju H G, Piao Z Y. Pollen mother cell meiosis and male gametophyte development of Atractylodes japonica[J]. Acta Botanica Boreali-Occidentalia Sinica, 2015, 35(8): 1547-1553.
[14]	Luan L, Tu S B, Long W B, Wang X, Liu Y H, Kong F L, He T, Yan W G, Yu M Q. Cytogenetic studies on two F1 hybrids of autotetraploid rice varieties showing extremely high level of heterosis[J]. Plant Systematics and Evolution, 2007, 267(4):205-213.doi:10.1007/s00606-007-0577-3. doi: 10.1007/s00606-007-0577-3 URL
[15]	Khazanehdari K A, Jones G H. The causes and consequences of meiotic irregularity in the leek(Allium ampeloprasum spp.porrum);implications for fertility,quality and uniformity[J]. Euphytica, 1997, 93(3):313-319.doi:10.1023/A:1002914808150. doi: 10.1023/A:1002914808150 URL
[16]	Weimarck A. Cytogenetic behaviour in octoploid Triticale.I.Meiosis,aneuploidy and fertility[J]. Hereditas, 1973, 74(1):103-118.doi:10.1111/j.1601-5223.1973.tb01108.x. doi: 10.1111/j.1601-5223.1973.tb01108.x URL
[17]	Atlagic' J, Dozet B, Škoric' D. Meiosis and pollen viability in Helianthus tuberosus L. and its hybrids with cultivated sunflower[J]. Plant Breeding, 1993, 111(4):318-324.doi:10.1111/j.1439-0523.1993.tb00648.x. doi: 10.1111/j.1439-0523.1993.tb00648.x URL
[18]	郭鲜蒲. 马铃薯体细胞杂种及其回交后代的遗传稳定性分析[D]. 武汉: 华中农业大学, 2011.doi: 10.7666/d.Y2162421. doi: 10.7666/d.Y2162421
	Guo X P. Genetic stability analysis of potato somatic hybrids and their pedigrees derived from backcrossing[D]. Wuhan: Huazhong Agricultural University, 2011.
[19]	李景伟, 于卓, 于肖夏, 李佳奇, 卢倩倩, 吴国芳, 李靓. 马铃薯优良杂种株系细胞遗传学特性及SRAP分析[J]. 东北师大学报(自然科学版), 2021, 53(2): 86-93.doi:10.16163/j.cnki.22-1123/n.2021.02.016. doi: 10.16163/j.cnki.22-1123/n.2021.02.016
	Li J W, Yu Z, Yu X X, Li J Q, Lu Q Q, Wu G F, Li L. The cytogenetics characteristics and SRAP analysis of potato excellent hybrid strains[J]. Journal of Northeast Normal University (Natural Science Edition), 2021, 53(2): 86-93.
[20]	王玲玲, 陈东亮, 黄丛林, 邢震. SSR分子标记技术在植物研究中的应用[J]. 安徽农业科学, 2017, 45(36): 123-126,130.doi:10.13989/j.cnki.0517-6611.2017.36.039. doi: 10.13989/j.cnki.0517-6611.2017.36.039
	Wang L L, Chen D L, Huang C L, Xing Z. Application of SSR molecular marker technique in plant research[J]. Journal of Anhui Agricultural Sciences, 2017, 45(36): 123-126,130.
[21]	罗兵, 孙海燕, 徐港明, 扬志刚, 沈宗根, 顾雯雯, 高阳, 郑佳亮. SSR分子标记研究进展[J]. 安徽农业科学, 2013, 41(12): 5210-5212,5246.doi:10.13989/j.cnki.0517-6611.2013.12.018. doi: 10.13989/j.cnki.0517-6611.2013.12.018
	Luo B, Sun H Y, Xu G M, Yang Z G, Shen Z G, Gu W W, Gao Y, Zheng J L. Research progress of SSR molecular marker[J]. Journal of Anhui Agricultural Sciences, 2013, 41(12): 5210-5212,5246.
[22]	凌晨, 赵洪, 颜军, 马莹雪, 冯艳芳, 张靖立, 李波, 邓超, 徐振江. 基于SSR标记的莴苣品种鉴定体系的建立及应用[J]. 华北农学报, 2022, 37(4): 34-44. doi: 10.7668/hbnxb.20192846. doi: 10.7668/hbnxb.20192846
	Ling C, Zhao H, Yan J, Ma Y X, Feng Y F, Zhang J L, Li B, Deng C, Xu Z J. Establishment and application of variety identification system based on SSR Markers for lettuce[J]. Acta Agriculturae Boreali-Sinica, 2022, 37(4): 34-44. doi: 10.7668/hbnxb.20192846
[23]	Duan Y F, Liu J, Xu J F, Bian C S, Duan S G, Pang W F, Hu J, Li G C, Jin L P. DNA fingerprinting and genetic diversity analysis with simple sequence repeat markers of 217 potato cultivars(Solanum tuberosum L.)in China[J]. American Journal of Potato Research, 2019, 96(1):21-32.doi:10.1007/s12230-018-9685-6. doi: 10.1007/s12230-018-9685-6 URL
[24]	高玉坤, 崔江慧, 项晓冬, 薛薇, 殷丛培, 任根增, 李峰, 王维东, 王志刚, 魏世林, 梁红凯, 常金华. 65个马铃薯品种(系)指纹图谱构建和遗传多样性分析[J]. 农业生物技术学报, 2020, 28(8): 1363-1378.doi:10.3969/j.issn.1674-7968.2020.08.004. doi: 10.3969/j.issn.1674-7968.2020.08.004
	Gao Y K, Cui J H, Xiang X D, Xue W, Yin C P, Ren G Z, Li F, Wang W D, Wang Z G, Wei S L, Liang H K, Chang J H. Fingerprint construction and genetic diversity analysis for 65 potato(Solanum tuberosum)varieties(lines)[J]. Journal of Agricultural Biotechnology, 2020, 28(8): 1363-1378.
[25]	宋洁, 郭华春, 李婉琳, 姚超. SSR分子标记分析CIP引进马铃薯品系与国内资源的遗传差异[J]. 南方农业学报, 2017, 48(4): 567-573.doi:10.3969/j.issn.2095-1191.2017.04.001. doi: 10.3969/j.issn.2095-1191.2017.04.001
	Song J, Guo H C, Li W L, Yao C. Genetic differences between potato strains introduced from International Potato Center(CIP)and domestic potato resources by SSR molecular markers[J]. Journal of Southern Agriculture, 2017, 48(4): 567-573.

引物名称 Primer name	正向引物(5'—3') Forward primer(5'—3')	反向引物(5'—3') Reversed primer(5'—3')
S7	GACTGGCTGACCCTGAACTC	GACAAAATTACAGGAACTGCAAA
S25	GCGAATGACAGGACAAGAGG	TGCCACTGCTACCATAACCA
S153	TATGTTCCACGCCATTTCAG	ACGGAAACTCATCGTGCATT
S174	TGAGGGTTTTCAGAAAGGGA	CATCCTTGCAACAACCTCCT
S189	CCTTGTAGTACAGCAGTGGTC	TCCGCCAAGACTGATGCA
C42	AGGCAAACCCCGTAAATCTT	TCTTCCTCACTCGTCCCACT
C60	ACTTCTGCATCTGGTGAAGC	GGTCGGATTCCCAGGTTG
STI014	AGAAACTGAGTTGTGTTGGGA	TCAACAGTCTCAGAAAACCCTCT
STM1016	TTCTGATTTCATGCATGTTTCC	ATGCTTGCCATGTGATGTGT
STM2022	GCGTCAGCGATTTCAGTACTA	TTCAGTCAACTCCTGTTGCG

引物名称 Primer name	正向引物(5'—3') Forward primer(5'—3')	反向引物(5'—3') Reversed primer(5'—3')
S7	GACTGGCTGACCCTGAACTC	GACAAAATTACAGGAACTGCAAA
S25	GCGAATGACAGGACAAGAGG	TGCCACTGCTACCATAACCA
S153	TATGTTCCACGCCATTTCAG	ACGGAAACTCATCGTGCATT
S174	TGAGGGTTTTCAGAAAGGGA	CATCCTTGCAACAACCTCCT
S189	CCTTGTAGTACAGCAGTGGTC	TCCGCCAAGACTGATGCA
C42	AGGCAAACCCCGTAAATCTT	TCTTCCTCACTCGTCCCACT
C60	ACTTCTGCATCTGGTGAAGC	GGTCGGATTCCCAGGTTG
STI014	AGAAACTGAGTTGTGTTGGGA	TCAACAGTCTCAGAAAACCCTCT
STM1016	TTCTGATTTCATGCATGTTTCC	ATGCTTGCCATGTGATGTGT
STM2022	GCGTCAGCGATTTCAGTACTA	TTCAGTCAACTCCTGTTGCG

材料 Materials	可育花粉粒数目 Pollen grain fertility number	总的花粉粒数目 Total pollen grain number	花粉可育率 /% Pollen grain fertility rate
NNCS-33	3 519	4 506	78.10
NNCS-37	4 103	5 115	80.22
NNCS-6	3 381	4 542	74.44
NNCS-7	3 651	6 015	60.70
NNCS-5	4 716	7 062	66.78
NNCS-1	2 506	5 696	44.00
MIN-021(CK)	3 869	5 144	75.21

材料 Materials	可育花粉粒数目 Pollen grain fertility number	总的花粉粒数目 Total pollen grain number	花粉可育率 /% Pollen grain fertility rate
NNCS-33	3 519	4 506	78.10
NNCS-37	4 103	5 115	80.22
NNCS-6	3 381	4 542	74.44
NNCS-7	3 651	6 015	60.70
NNCS-5	4 716	7 062	66.78
NNCS-1	2 506	5 696	44.00
MIN-021(CK)	3 869	5 144	75.21

引物名称 Primer name	多态性条带 Number of polymorphic loci	扩增条带总数 Number of amplified loci	多态性比率 /% Percentage of polymorphic loci
S7	12	14	85.71
S25	13	16	81.25
S153	16	18	88.89
S174	13	15	86.67
S189	16	17	94.12
C42	23	24	95.83
C60	13	17	76.47
STI014	13	16	81.25
STM2022	15	18	83.33
STM1016	17	19	89.47
总计Total	151	174	86.78

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