ACTA AGRICULTURAE BOREALI-SINICA ›› 2012, Vol. 27 ›› Issue (6): 72-77. doi: 10.3969/j.issn.1000-7091.2012.06.016

Special Issue: Potato crops

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SSR Analysis on Genetic Diversity of Leading Potato Cultivars in Shanxi Province

LIU Jian-xia1, LEI Hai-ying2, WEN Ri-yu3, GUO Yao-dong3, WANG Run-mei1, ZHOU Feng1   

  1. 1. College of Agronomy & Life Science, Shanxi Datong University, Datong 037009, China;
    2. Department of Biological Sciences and Technology, Changzhi College, Changzhi 046011, China;
    3. Institute of Maize, Shanxi Academy of Agricultural Sciences, Xinzhou 034000, China
  • Received:2012-09-03 Published:2012-12-28

Abstract: In this paper, the genetic diversities of 12 parts key potato cultivars in Shanxi province were analyzed by SSR markers. Eleven pairs of SSR primers with high polymorphism and clear bands were selected from 60 pairs of SSR primers. Among 79 SSR bands obtained from 11 primers, 77 bands were polymorphic accounting for 97. 5% of the total. The differences in SSR DNA fingerprints amplified with three primers S189,S151 and STM001 were significant. It could be used as molecular evidence to distinguish 12 potato varieties. The genetic distance of twelve potato cultivars ranged from 0. 209 2 to 0. 626 9,and the average value was 0. 421 7. At 0. 632 of genetic similarity, the 12 materials were clustered into 4 groups, the first group had three varieties( Feierruita,Atlantic and Jizhangshu-No. 8),Tongshu 24,Zihuabai were clustered into the third and the fourth groups separately. The other varieties, which bred in Shanxi province and showed a close genetic relationship,were clustered into the second group. Results showed that the genetic basis of the existing potato cultivars in Shanxi province is relatively narrow and the interspecies differences of these cultivars are not significant.

Key words: Potato, Main cultivars, Genetic diversity, SSR analysis, Cluster analysis

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Cite this article

LIU Jian-xia, LEI Hai-ying, WEN Ri-yu, GUO Yao-dong, WANG Run-mei, ZHOU Feng. SSR Analysis on Genetic Diversity of Leading Potato Cultivars in Shanxi Province[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(6): 72-77. doi: 10.3969/j.issn.1000-7091.2012.06.016.

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