Construction of Core Collection of Yanshan Chestnut Germplasm Based on SSR Markers

doi:10.7668/hbnxb.20192435

Abstract

Abstract: The suitable means to construct core collection of Yanshan chestnut germplasm based on SSR makers was studied, which was beneficial for preservation, management and utilization of chestnut germplasm resources.Taking 161 Yanshan chestnut germplasm resources from 10 cities(counties)as original materials, an allele-preferred sampling method and random sampling strategy were compared using UPGMA cluster method according to 3 genetic similarity coefficients(SM, Dice and Jaccard coefficient)based on SSR molecular markers. The effective number of alleles(Ne), Nei's gene diversity(H)and Shannon's information index(I)of two groups were compared to determine the optimum method. t -tests of core collection, initial collection and reserve collection were conducted to evaluate the representativeness of core collection. The principle coordinate analysis method and phonotypic traits analysis were used to confirm on the core collection.Compared with the random sampling strategy, allele preferred sampling method was more representative with higher values of genetic diversity indexes. The results showed the method of stepwise clustering according to SM similarity coefficient was better than Dice coefficient and Jaccard coefficient. 46 core collection included 28.57% initial collection. The effective number of alleles, Nei's gene diversity, Shannon's information index were respectively 1.531 7, 0.321 8 and 0.491 0. t -test showed the genetic diversity index of core collection was significantly higher that of initial collection.The core collection was evenly distributed in the principle coordinate diagram of initial collection, which could represent genetic diversity of the whole chestnut germplasm comprehensively.The method of allele preferred sampling and stepwise clustering according to SM similarity coefficient was a suitable means to construct core collection of Yanshan chestnut germplasm based on SSR makers, and 46 core collection could represent genetic diversity of original germplasm resources.

Key words: Yanshan chestnut, Core collection, SSR, Genetic diversity

CLC Number:

S664.2

ZHANG Xinfang, ZHANG Shuhang, LI Ying, GUO Yan, WANG Guangpeng. Construction of Core Collection of Yanshan Chestnut Germplasm Based on SSR Markers[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(S1): 31-38. doi: 10.7668/hbnxb.20192435.

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