Genetic Diversity Analysis of Macrobrachium nipponense from Dalonghupao

doi:10.7668/hbnxb.20193703

Abstract

Abstract:

To learn more about the genetic characteristics of Macrobrachium nipponense and provide theoretical support for breeding selection,it used microsatellite molecular marking technology to compare the genetic diversity of Macrobrachium nipponense in Dalonghupao. The results showed that all 12 microsatellite loci were highly polymorphic loci (PIC>0.5). In the population of Taihu 2,the observed number of alleles ranged from 4 to 11,the effective number of alleles ranged from 3.686 4 to 7.480 5,the observed heterozygosity was 0.318 2—0.833 3,the expected heterozygosity was 0.744 9—0.884 8,the PIC of each locus was 0.691 9—0.851 7. The population of Taihu 2 was with high genetic diversity. In the population of Dalonghupao,the observed number of alleles ranged from 1—2,the effective number of alleles was 1.000 0—2.000 0,the observed heterozygosity was 0.000 0—1.000 0,the expected heterozygosity was 0.000 0—0.517 2,the PIC of each locus was 0.000 0—0.375 0. The population of Dalonghupao was with low genetic diversity. Seven loci in both populations deviated significantly from Hardy-Weinberg equilibrium,and three loci in the population of Dalonghupao could not be tested for Hardy-Weinberg equilibrium. Seven loci in the population of Taihu 2 showed heterozygous deletion,while seven loci in the population of Dalonghupao showed heterozygous excess. AMOVA analysis showed that 36.15% (P<0.01)of the total genetic variation was among populations and 63.85% (P<0.01)was among individuals. The genetic similarity between the population of Dalonghupao and the population of Taihu 2 was 0.276 9 and the genetic distance was 1.284 3,which showed that the genetic similarity between the two populations was low,and the genetic distance was far,and there were great genetic differences. The genetic differentiation index (F_ST)of the two populations was 0.361 53 (P<0.01),and the degree of genetic differentiation was obvious. The population genetic structure analysis showed an optimal K value of 3,indicating that the two populations had three possible ancestors. The level of genetic diversity of Dalonghupao wild population was low,which prompting us to protect the germplasm resources of the population of Dalonghupao.

Key words: Macrobrachium nipponense, Genetic diversity, Microsatellite, Taihu 2, Molecular marker

LAN Ruolin, MA Wenzhi, ZHANG Limin, SUN Bo, CHANG Yumei, LIANG Liqun. Genetic Diversity Analysis of Macrobrachium nipponense from Dalonghupao[J]. Acta Agriculturae Boreali-Sinica, 2023, 38(S1): 437-443. doi: 10.7668/hbnxb.20193703.

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References 30

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位点 Locus	基因序列号 GenBank accession number	引物来源文献 Source literature of primers
WXM25	GU189621	Qiao等^[10]
WXM33	GU189629
Mni001	EU130924	Feng等^[11]
Mni004	EU130926
Mni006	EU130928
Mni013	EU130932
Mni034	GQ257531	Ma等^[12]
Mni040	GQ257537
Mni051	GQ257548
Mni058	GQ257555
Mni076	GQ257573
X781	KF601809	Zhao等^[13]
XZ339	KF601824

位点 Locus	基因序列号 GenBank accession number	引物来源文献 Source literature of primers
WXM25	GU189621	Qiao等^[10]
WXM33	GU189629
Mni001	EU130924	Feng等^[11]
Mni004	EU130926
Mni006	EU130928
Mni013	EU130932
Mni034	GQ257531	Ma等^[12]
Mni040	GQ257537
Mni051	GQ257548
Mni058	GQ257555
Mni076	GQ257573
X781	KF601809	Zhao等^[13]
XZ339	KF601824

位点 Locus	等位基因数 Allele number	有效等位基因数 Number of effective allele	观测杂合度 Observed heterozygosity	期望杂合度 Expected heterozygosity	多态信息含量 Polymorphism information content
WXM25	7	5.643 8	0.769 2	0.833 5	0.799 0
WXM33	6	3.846 4	0.708 3	0.747 8	0.702 9
Mni001	9	5.358 1	0.750 0	0.821 9	0.791 6
Mni004	5	4.161 3	0.739 1	0.768 0	0.720 8
Mni006	8	4.169 5	0.847 8	0.768 5	0.725 9
Mni013	9	5.992 5	0.675 0	0.843 7	0.813 1
Mni034	6	2.733 9	0.191 5	0.641 0	0.602 4
Mni051	9	2.878 2	0.191 5	0.659 6	0.614 9
Mni058	11	3.400 7	0.416 7	0.713 4	0.687 6
Mni076	10	4.170 1	0.666 7	0.768 2	0.726 5
X781	4	3.013 7	0.541 7	0.675 2	0.619 1
XZ339	7	4.951 1	0.666 7	0.807 0	0.769 8
平均Mean	7.583 3	4.193 3	0.597 0	0.754 0	0.714 5

位点 Locus	等位基因数 Allele number	有效等位基因数 Number of effective allele	观测杂合度 Observed heterozygosity	期望杂合度 Expected heterozygosity	多态信息含量 Polymorphism information content
WXM25	7	5.643 8	0.769 2	0.833 5	0.799 0
WXM33	6	3.846 4	0.708 3	0.747 8	0.702 9
Mni001	9	5.358 1	0.750 0	0.821 9	0.791 6
Mni004	5	4.161 3	0.739 1	0.768 0	0.720 8
Mni006	8	4.169 5	0.847 8	0.768 5	0.725 9
Mni013	9	5.992 5	0.675 0	0.843 7	0.813 1
Mni034	6	2.733 9	0.191 5	0.641 0	0.602 4
Mni051	9	2.878 2	0.191 5	0.659 6	0.614 9
Mni058	11	3.400 7	0.416 7	0.713 4	0.687 6
Mni076	10	4.170 1	0.666 7	0.768 2	0.726 5
X781	4	3.013 7	0.541 7	0.675 2	0.619 1
XZ339	7	4.951 1	0.666 7	0.807 0	0.769 8
平均Mean	7.583 3	4.193 3	0.597 0	0.754 0	0.714 5

位点 Loci	等位基因数 Allele number		有效等位基因数 Number of effective allele		观测杂合度 Observed heterozygosity		期望杂合度 Expected heterozygosity		P值 P-value		多态信息含量 Polymorphism information content
位点 Loci	太湖 2号 TH	大龙虎泡 DM	太湖 2号 TH	大龙虎泡 DM	太湖 2号 TH	大龙虎泡 DM	太湖 2号 TH	大龙虎泡 DM	太湖 2号 TH	大龙虎泡 DM	太湖 2号 TH	大龙虎泡 DM
WXM25	7	2	5.142 9	2.000 0	0.625 0	1.000 0	0.822 7	0.517 2	0.028 3	0.000 3^**	0.778 0	0.375 0
WXM33	6	2	4.282 5	1.916 8	0.625 0	0.791 7	0.782 8	0.488 5	0.008 8	0.002 2^*	0.729 0	0.363 9
Mni001	9	2	7.480 5	1.986 2	0.583 3	0.916 7	0.884 8	0.507 1	0.000 0^**	0.000 0^**	0.851 7	0.373 3
Mni004	5	2	4.100 8	2.000 0	0.478 3	1.000 0	0.772 9	0.511 1	0.004 2	0.000 0^**	0.715 8	0.375 0
Mni006	8	2	5.866 7	1.986 2	0.772 7	0.916 7	0.848 8	0.507 1	0.274 8	0.000 0^**	0.807 5	0.373 3
Mni013	9	2	6.471 9	1.969 2	0.541 7	0.875 0	0.863 5	0.508 1	0.000 4^**	0.004 2	0.829 7	0.371 1
Mni034	6	1	4.723 2	1.000 0	0.391 3	0.000 0	0.805 8	0.000 0	0.000 0^**	-	0.758 7	0.000 0
Mni051	9	1	3.686 4	1.000 0	0.391 3	0.000 0	0.744 9	0.000 0	0.000 0^**	-	0.707 9	0.000 0
Mni058	11	1	7.432 3	1.000 0	0.833 3	0.000 0	0.883 9	0.000 0	0.023 9	-	0.851 0	0.000 0
Mni076	10	2	6.816 6	2.000 0	0.333 3	1.000 0	0.871 5	0.510 6	0.000 0^**	0.000 0^**	0.837 4	0.375 0
X781	4	2	3.852 8	1.704 1	0.500 0	0.583 3	0.756 2	0.422 0	0.002 2^*	0.066 2	0.691 9	0.327 8
XZ339	7	2	4.913 7	2.000 0	0.318 2	1.000 0	0.815 0	0.511 1	0.000 0^**	0.000 0^**	0.766 6	0.375 0
平均Mean	7.583 3	1.713 5	5.397 5	0.509 3	0.532 8	0.626 4	0.821 1	0.364 7	-	-	0.777 1	0.275 8

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