Correlation Study on Polymorphism of GnIH with Egg-Laying Traits and Body Measurement Traits in Goose

doi:10.7668/hbnxb.20192256

Abstract

Abstract: To investigate the polymorphism of GnIH gene association with egg-laying or body measurement traits in Goose, we cloned the GnIH DNA sequence, and analysis the character of the genes with that of mammal and poultry. Moreover, we detected the GnIH DNA sequence in Sichuan White goose populations (208 individuals), and performed the association with the polymorphism of the GnIH gene with egg-laying and body composition traits. In this study, we cloned a 3 735 bp genomic sequence and a 522 bp mRNA sequence for GnRH, consisting with 3 exons and 2 introns, code 173 amino acid pre-protein. Genetic evolution analysis had shown that the similarity of GnIH gene sequence between Sichuan white goose and mallard duck was the highest (99%). Using a direct sequencing method, 26 single nucleotide polymorphisms (SNPs) of GnIH genomic sequence were obtained, and the association analysis has revealed that the 11 SNPs(g.811T>C, g.1892T>C, g.1320G>A, g.1809G>T, g.2164C>G, g.1708A>G, g.2164C>G, g.1809G>T, g.1892T>C, g.1929C>A和g.2164C>G) were significant or extremely significant associated with five traits, including first egg weight (FEW), body length (BL), neck length (NL), chest width (CW) and shank circumferences (SC) (P<0.05 or P<0.01). However, there was no significant correlation with egg number at 48 weeks and 64 weeks, birth weight, semi-submersible length, keel length and pelvic width (P>0.05). In conclusion, we cloned 3 735 bp goose GnIH DNA genomic sequence, and the 11 of the 26 SNPs of of the GnIH gene can be used as one of the potential molecular markers of the traits such as first-laying egg quality, body length, neck length, chest width and tibial girth.

Key words: Goose, GnIH gene, SNPs, Egg-laying traits, Body measurement traits, Molecular marker selection

CLC Number:

S835.2

GAO Guangliang, ZHANG Keshan, XU Guoyang, ZHAO Xianzhi, CHEN Zhuping, ZHOU Li, WANG Qigui. Correlation Study on Polymorphism of GnIH with Egg-Laying Traits and Body Measurement Traits in Goose[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(5): 204-210. doi: 10.7668/hbnxb.20192256.

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