Molecular Characterization and Tissue Expression Analysis of HCRT and HCRTR2 Genes in Goose

doi:10.7668/hbnxb.201751344

Abstract

Abstract: The aim of this study was to clone the complete coding sequences of HCRT and HCRTR2 genes in goose by designing the specific primers according to their respective reference sequences of humans, chicken and ducks on NCBI website, and then they were analyzed by corresponding bioinformatic method. The expression levels of HCRT and HCRTR2 mRNA in nineteen tissues of Sichuan White Goose were detected. The complete coding regions of HCRT and HCRTR2 genes in goose were 456,1 506 bp, respectively, encoding 151 and 501 amino acids. Among them, the precursor orexin protein encoded by HCRT gene was hydrolyzed to produce OXA and OXB polypeptides. Sequence analysis showed that goose OXA and OXB contained 34 and 28 amino acid residues, respectively. The sequence alignment showed that the amino acid sequences of OXA, OXB and HCRTR2 were highly conserved among the nearest species, suggesting that OXA, OXB and HCRTR2 might play an important role in the evolution of species. However, the signal peptide conservatism of orexin protein among species was low, suggesting that the signal peptide sequence of orexin protein might be important to the functional characteristics of species. The evolutionary tree analysis showed that the evolution of HCRT and HCRTR2 were similar. Goose had the closest relationship with chicken and the farthest with zebra fish. RT-qPCR results showed that the expression levels of HCRT and HCRTR2 genes in the hypothalamus were the highest, though they were also expressed in other tissues to a certain degree. It is speculated that orexin system might participate in the regulation of animal reproductive function through hypothalamus-pituitary-gonad axis (HPG). The results of this study laid the foundation for further studying the role of HCRT and HCRTR2 genes in goose reproductive activities.

Key words: Goose, HCRT, HCRTR2, Gene cloning, Tissue expression

CLC Number:

S835

OUYANG Qingyuan, LIANG Jinmin, WANG Yushi, GAN Xiang, HU Shenqiang, WANG Jiwen. Molecular Characterization and Tissue Expression Analysis of HCRT and HCRTR2 Genes in Goose[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(3): 224-231. doi: 10.7668/hbnxb.201751344.

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