Characterization and Differential Expression Profile of Calm1 Gene in Sichuan White Goose

doi:10.7668/hbnxb.2016.01.021

Abstract

Abstract: In order to elucidate the characteristics and expression profile of Calm1 in ovarian hierarchical follicles and tissues of Sichuan white goose,the Calm1 coding region sequence of the goose was clone,and it's structure and function was characterized.The relative abundance of Calm1 mRNA in ovarian follicles and various tissues of Sichuan white goose were assessed by quantitative Real-time PCR.The results showed that the Calm1 coding sequence was 450 bp with encoding 149 amino acid residues.The sequence of the goose Calm1 amino acids shared a 99% similarity compared to Gallus gallus.The level of Calm1 gene expression in cerebrum was significantly higher than others (P<0.05). Calm1 transcripts were detectable in all of the experimental follicles.The level of Calm1 gene expression in F2 was 1.76-fold compared with small white follicles,and was significantly higher than F5,F4,F3 and F1 level follicle (P<0.05).The level of Calm1 in postovulatory follicle 1 was significantly higher than POF2,POF3 and POF4 (P<0.05).The Calm1 expression level in ovarian tissue was 1.75-fold compared with small white follicles (P<0.05).Calm1 is a highly conserved ubiquitous protein.Calm1 may participate in regulating the animal reproduction by affecting the growth of follicular cell proliferation and synthesis of steroid hormones.

Key words: Sichuan white goose, Quantitative Real-time PCR, Calm1, Gene expression

CLC Number:

S858.03

XU Qilin, HE Hui, MA Rong, CHEN Ziyu, YI Zhixin, KANG Bo, JIANG Dongmei. Characterization and Differential Expression Profile of Calm1 Gene in Sichuan White Goose[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(1): 128-133. doi: 10.7668/hbnxb.2016.01.021.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2016.01.021

http://www.hbnxb.net/EN/Y2016/V31/I1/128

References

[1] Toutenhoofd S L,Strehler E E.Regulation of calmodulin mRNAs in differentiating human IMR-32 neuroblastoma cells[J].Biochimica et Biophysica acta,2002,1600(1/2):95-104.
[2] 赵岚,陆融,姚智.Ca²⁺/钙调蛋白依赖性蛋白激酶在细胞增殖中的作用[J].细胞生物学杂志,2007,29(3):331-335.
[3] Feng R,Xu J,Minobe E,et al.Adenosine triphosphate regulates the activity of guinea pig Cav1.2 channel by direct binding to the channel in a dose-dependent manner[J]. American Journal of Physiology-Cell Physiology,2014,306(9):856-863.
[4] Yu F H,Jun H L,Yuan S Q.Roles of calmodulin-dependent protein kinase Ⅱ in meiotic maturation and fertilization of oocytes[J].Progress in Biochemistry and Biophysics,2003,30(2):171-174.
[5] 柳淑芳,杜立新,王继英.哺乳动物卵母细胞发育机理的研究进展[J].黄牛杂志,2001,27(4):43-45.
[6] 王晨光,邓满齐,孙方臻.小鼠卵母细胞体外成熟不同阶段对钙的依赖性[J].实验生物学报,1998,31(2):147-153.
[7] Levorse J M,Tilly J L,Johnson A L.Role of Calcium in the regulation of theca cell androstenedione production in the domestic hen[J].Journal of Reproduction and Fertility,1991,92(1):159-167.
[8] Kobayashi H,Saragai S,Naito A,et al.Calm1 signaling pathway is essential for the migration of mouse precerebellar neurons[J].Development,2015,142(2):375-384.
[9] Flanagan J M,Popendikyte V,Pozdniakovaite N,et al.Intra-and interindividual epigenetic variation in human germ cells[J].American Journal of Human Genetics,2006,79(1):67-84.
[10] Kang B,Guo J R,Yang H M,et al.Differential expression profiling of ovarian genes in prelaying and laying geese[J].Poultry Science,2009,88(9):1975-1983.
[11] Ji H,Wang J,Guo J,et al.Expression of eleven egg performance-associated genes in the ovary of Zi geese anser anser domestica[J].The Journal of Poultry Science,2013,50(2):91-98.
[12] Ignotz G G,Suarez S S.Calcium/calmodulin and calmodulin kinase Ⅱ stimulate hyperactivation in demembranated bovine sperm[J].Biology of Reproduction,2005,73(3):519-526.
[13] 康波,姜冬梅,郭静茹,等.籽鹅卵巢组织差异表达基因的研究[J].畜牧兽医学报,2010,41(6):657-663.
[14] Luan J,Liu Z,Zhang S,et al.Characterization,evolution and expression of the calmodulin1 genes from the amphioxus Branchiostoma belcheri tsingtauense[J].Acta Biochimica et Biophysica Sinica,2007,39(4):255-264.
[15] 封瑞,刘彦,杨磊,等.豚鼠钙调蛋白2基因编码区及3'端非编码区序列克隆[J].中国医科大学学报,2015,44(2):123-126.
[16] Kang S,Xu M,Cooper E C,et al.Channel-anchored protein kinase CK2 and protein phosphatase 1 reciprocally regulate KCNQ2-containing M-channels via phosphorylation of calmodulin[J].The Journal of Biological Chemistry,2014,289(16):11536-11544.
[17] Friedberg F,Rhoads A R.Evolutionary aspects of calmodulin[J].IUBMB Life,2001,51(4):215-221.
[18] Grabarek Z.Insights into modulation of calcium signaling by magnesium in calmodulin,troponin C and related EF-hand proteins[J].Biochimica et Biophysica acta,2011,1813(5):913-921.
[19] 袁俊,李朝军.钙调蛋白在PC12细胞分化和迁移中的作用[J].华北农学报,2011,26(1):22-25.
[20] Rhyner J A,Ottiger M,Wicki R,et al.Structure of the human CALM1 calmodulin gene and identification of two CALM1-related pseudogenes CALM1P1 and CALM1P2[J].European Journal of Biochemistry,1994,225(1):71-82.
[21] Ye Q,Berchtold M W.Structure and expression of the chicken calmodulin Ⅰ gene[J].Gene,1997,194(1):63-68.
[22] Chien Y H,Dawid I B.Isolation and characterization of calmodulin genes from Xenopus laevis[J].Molecular and Cellular Biology,1984,4(3):507-513.
[23] Matsuura T,Sugimura M,Iwaki T,et al.Anti-macrophage inhibitory factor antibody inhibits PMSG-hCG-induced follicular growth and ovulation in mice[J].Journal of Assisted Reproduction and Genetics,2002,19(12):591-595.
[24] 郑亦辉,张德福.湖羊与美利奴羊卵巢不同直径的卵泡钙调素含量的变化[J].南京农业大学学报,1990,13(3):128-129.
[25] 应诗家,彭中友,李燕,等.绵羊黄体期卵巢类固醇激素调节基因的表达研究[J].畜牧兽医学报,2013,44(11):1775-1780.
[26] Manière G,Vanhems E,Gautron F,et al.Calcium inhibits ovarian steroidogenesis in the blowfly Phormia regina[J].The Journal of Endocrinology,2002,173(3):533-544.
[27] Jayes F C,Day R N,Garmey J C,et al.Calcium ions positively modulate follicle-stimulating hormone-and exogenous cyclic 3',5'-adenosine monophosphate-driven transcription of the P450 (scc) gene in porcine granulosa cells[J].Endocrinology,2000,141(7):2377-2384.
[28] 何俊,陈建锋,刘茹,等.氰戊菊酯对卵巢细胞、组织钙稳态和激素水平的影响[J].中华预防医学杂志,2004,38(1):18-21,73.
[29] Golini V E,Stradaioli G,Sirard M A.Transcriptome analysis of bovine granulosa cells of preovulatory follicles harvested 30,60,90,and 120 days postpartum[J].Theriogenology, 2014,82(4):580-591.

Please choose a citation manager

Content to export