Response Analysis of Rice Gene NAL11 to Abiotic Stresses at the Stage of Seedling

doi:10.7668/hbnxb.20190988

Abstract

Abstract: In order to explore the response mechanism and the function of gene NAL11 to abiotic stress at the stage of rice seedling,we treated the Oryza sativa L. subsp. Japonica cv. 02428,contained gene NAL11,and its near-isogenic line NIL-8 with high temperature,low temperature,salt stress and simulated drought stress,respectively,with the plants under normal conditions as control. In the case,the phenotypes and some physiological indices of the plants under each treatment were observed and assayed. Then,we compared the expressions of gene NAL11 under different stress treatments,and some phenomena were found to be interesting. Firstly,under the independent treatments of high temperature,low temperature and salt stress,02428 and NIL-8 showed a similar degree of wilting and drying symptoms,which were very mild. However,when treated with 15% PEG-6000,leaves of 02428 and NIL-8 turned wilted,and the leaf tips became yellow and curly,but the symptoms of NIL-8 were relatively slighter than 02428. Besides,NIL-8 showed lower relative conductivity and higher SPAD value than 02428. Meanwhile,compared with 02428,there was no significant change in SOD activities of NIL-8,but the CAT activities of NIL-8 was significantly higher than that of 02428 under low temperature and drought stress. The above results indicated that NIL-8 possessed a better drought resistance than 02428. From the Real-time quantitative PCR,we could see that the expression of gene NAL11 increased rapidly after treated with high temperature,low temperature,salt stress and simulated drought stress individually,and the expression changed dynamically along with the extension of treatment time. Thus,it is concluded that the expression of gene NAL11 would be induced by high temperature,low temperature,salt stress and simulated drought stress,but the expression patterns were different. So it is reasonable for us to suppose that gene NAL11 plays a certain role in the drought resistance of rice seedlings.

Key words: Rice, Heat shock protein, Abiotic stress, Gene expression

CLC Number:

S511.01

WANG Changlong, ZHANG Li, LUO Lixin, WANG Hui, GUO Tao, LIU Yongzhu, ZHOU Jiyong, CHEN Zhiqiang, XIAO Wuming. Response Analysis of Rice Gene NAL11 to Abiotic Stresses at the Stage of Seedling[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(4): 120-128. doi: 10.7668/hbnxb.20190988.

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