Abstract: In order to study the relationship between the PRKAG3 gene polymorphism and slaughter performance,meat quality of 5 sheep breeds(Gansu alpine fine wool sheep,Small tail han sheep,Tibetan,Mongolian sheep and Tan-sheep which were fed in a typical farming mode of Gansu province) in Gansu province at the molecular level and to explore the possibility of improving the slaughter performance and quality of sheep meat by using PRKAG3 as candidate gene,the polymorphism of exon 4,5and introns 4,a total of 321 bp,of PRKAG3 gene was detected by PCR-SSCP in 5 sheep breeds,and the association between PRKAG3 gene polymorphism and slaughter performance,meat quality in aged 9 month were analyzed.The results showed:that B allele had a base transformation of A>G at the 2 689th bp in intron 4 formed AA,AB and BB genetypes.The gene homozygosity (Ho)were 0.67,0.69,0.76,0.69 and 0.59 respectively;the heterozygosity were 0.333 3,0.306 1,0.244 9,0.307 7 and 0.408 2,respectively;the effective number of alleles (Ne) were 1.62,1.66,1.69,1.48 and 1.79;the polymorphism information content (PIC) were 0.31,0.32,0.33,0.27 and 0.34,respectively.In addition to the Tibetan sheep other four sheep breeds on this loci in a Hardy-Weinberg equilibrium state by Chi-square test.Association analysis of the SNPs with meat quality traits was carried out.The result showed that the mutation loc of PRKAG3 gene was significantly associated (P<0.05) with meat water loss rate,genotype BB between individual's body weight was significantly higher than AA type (P<0.05),Tan-sheep was very significant(P<0.01).The eye muscle area was significantly greater between a BB genotype and AA type (P<0.05).So, PRKAG3 gene can be used as a candidate gene for marker-assisted selection of sheep meat performance and meat quality.

Key words: Sheep, PCR-SSCP, PRKAG3 gene, Meat quality traits, Association analysis

CLC Number: 

• Q78

• S858.03