Association Analysis of SSR Markers and Photoperiod Sensitivity in Foxtail Millet

doi:10.7668/hbnxb.201750672

Abstract

Abstract: Sensitivity to photoperiod is the main reason for narrow ecological adaptability and lacking cross-regional cultivars of foxtail millet. Identification of QTLs associated with millet photoperiod sensitivity is the foundation for further exploring genetic mechanisms of photoperiod sensitivity. 45 millet cultivars were surveyed for heading date in long-day region (Luoyang City, Henan Province) and short-day region (Ledong County, Hainan Province). The photoperiod sensitivity values of 45 millet cultivars were calculated according to the difference between the heading date in the two regions. Then, 40 pairs of SSR markers were used to determine the genotypes of these millet cultivars. Finally association analysis between SSR markers and photoperiod sensitivity was performed. The results showed that a total of 150 alleles were detected in 45 millet cultivars, giving an average allele number of 3.75 per locus. Of the 40 SSR markers, b200 and b124 amplified the most number of alleles (6 alleles per locus). Based on the SSR markers amplification data, the population genetic structure of 45 millet cultivars was analyzed, and the optimal K value was 3, so the 45 millet cultivars were divided into three subgroups. The first subgroup included 5 cultivars, the second subgroup included 16 cultivars and the third subgroup included 21 cultivars. The remaining three cultivars could not be grouped into any of the three subgroups, consisted of a mixed group. The linkage disequilibrium analysis showed that no significant linkage disequilibrium structures were found among 40 SSR markers. Finally association analysis between SSR markers and photoperiod sensitivity were performed, two loci, b200 (SSR8) and b127 (SSR35) were detected to associate with photoperiod sensitivity(P<0.05).

Key words: Millet, Photoperiod sensitivity, SSR markers, Association analysis

CLC Number:

S515.01

JIA Xiaoping, ZHANG Bo, LI Jianfeng, YUAN Xilei, LU Ping, DAI Lingfeng. Association Analysis of SSR Markers and Photoperiod Sensitivity in Foxtail Millet[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(1): 89-96. doi: 10.7668/hbnxb.201750672.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.201750672

http://www.hbnxb.net/EN/Y2019/V34/I1/89

References

[1] 邓杰, 戴林建, 杨苏, 周田. 光温条件对烤烟生长发育和品质形成的影响综述[J]. 作物研究, 2015, 9(6):678-681. doi:10.3969/j.issn.1001-5280.2015.06.26. Deng J, Dai L J, Yang S, Zhou T. Research progress on effect of light and temperature on the growth and quality of flue-cured tobacco[J]. Crop Research, 2015,9(6):678-681. doi:10.3969/j.issn.1001-5280.2015.06.26.
[2] Garner W W, Allard H A. Effect of relative length of day and night and other factors of the environment on growth and reproduction in plants[J].J Agric Res,1920,18:553-606.
[3] 唐志明, 李晓方, 庄国富, 金晓春, 卢东柏. 水稻株高差异与光温生态敏感性的关系初探[J]. 广东农业科学, 2007(4):7-10. doi:10.3969/j.issn.1004-874X.2007.04.002. Tang Z M, Li X F, Zhuang G F, Jin X C, Lu D B. Searching for the correlative factors of photosensitiveness and temperature sensibility in rice[J]. Guangdong Agricultural Sciences, 2007(4):7-10. doi:10.3969/j.issn.1004-874X.2007.04.002.
[4] 王翠玲, 程芳芳, 孙朝晖, 库丽霞, 陈晓, 陈彦惠. 玉米光周期敏感性的遗传特性及相关基因的研究进展[J]. 玉米科学, 2008, 16(1):11-14, 19. Wang C L, Cheng F F, Sun C H, Ku L X, Chen X, Chen Y H. Advances in genetic research and related genes of photoperiod sensitivity in maize[J]. Journal of Maize Science, 2008,16(1):11-14, 19.
[5] 杨玉杰, 李海云. 光周期对植物生长发育的影响研究进展[J]. 安徽农业科学, 2010, 38(13):6693-6694, 6740. doi:10.3969/j.issn.0517-6611.2010.13.026. Yang Y J, Li H Y. Research progress on the effect of photoperiod on plant growth and development[J]. Journal of Anhui Agricultural Science, 2010,38(13):6693-6694, 6740. doi:10.3969/j.issn.0517-6611.2010.13.026.
[6] 沈韫赜, 郭双生. 受控环境下光周期对小麦生长发育、产量及营养品质的影响[J]. 麦类作物学报, 2015, 35(1):64-70. doi:10.7606/j.issn.1009-1041.2015.01.10. Shen Y Z, Guo S S. Effects of photoperiod on growth,development,yield and nutritional quality of wheat in controlled system[J]. Journal of Triticeae Crops, 2015,35(1):64-70. doi:10.7606/j.issn.1009-1041.2015.01.10.
[7] 李雪, 温昕, 邵铁梅, 孔卫娜, 刘培, 焦展, 安胜军. 芝麻遗传工程的研究进展[J]. 中国农学通报, 2012, 28(6):153-158. doi:10.3969/j.issn.1000-6850.2012.06.029. Li X, Wen X, Shao T M, Kong W N, Liu P, Jiao Z, An S J. Progress on genetic engineering of sesame[J]. Chinese Agricultural Science Bulletin, 2012,28(6):153-158. doi:10.3969/j.issn.1000-6850.2012.06.029.
[8] 赵治海, 崔文生, 杜贵, 杨少青. 谷子光(温)敏不育系821选育及其不育性与光温关系的研究[J]. 中国农业科学, 1996, 29(5):23-31. Zhao Z H, Cui W S, Du G, Yang S Q. The selection of millet photo(thermo) sensitive sterile line 821 and a study on the relation of sterility to illumination and temperature[J]. Scientia Agricultura Sinica, 1996,29(5):23-31.
[9] 贾小平, 袁玺垒, 李剑峰, 张博, 张小梅, 郭秀璞, 陈春燕. 不同光温条件谷子资源主要农艺性状的综合评价[J]. 中国农业科学, 2018, 51(13):578-1752. doi:10.3864/j.issn.0578-1752.2018.13.001. Jia X P, Yuan X L, Li J F, Zhang B, Zhang X M, Guo X P, Chen C Y. Comprehensive evaluation of main agronomic traits of millet resources under different light and temperature conditions[J]. Scientia Agricultura Sinica, 2018,51(13):578-1752. doi:10.3864/j.issn.0578-1752.2018.13.001.
[10] 贾小平, 李剑峰, 全建章, 王永芳, 董志平, 张博, 袁玺垒. 不同光周期条件下谷子农艺性状的光周期敏感性评价[J]. 植物遗传资源学报, 2018, 19(5):919-924. doi:10.13430/j.cnki.jpgr.20180207005. Jia X P, Li J F, Quan J Z, Wang Y F, Dong Z P, Zhang B, Yuan X L. Evaluation of photoperiod sensitivity of agronomic traits of foxtail millet varieties(Setaria italica) under different photoperiod conditions[J]. Journal of Plant Genetic Resources, 2018,19(5):919-924. doi:10.13430/j.cnki.jpgr.20180207005.
[11] 谢丽莉. 谷子光周期敏感相关性状的QTL定位与分析[D]. 郑州:河南农业大学, 2012. Xie L L. QTL mapping and analysis of photoperiod sensitivity of agronomic traits of foxtail millet varieties(Setaria italica)[D].Zhengzhou:Henan Agricultural University, 2012.
[12] Margarita Mauro-Herrera, Wang X W, Hugues Barbier, Thomas Brutnell, Katrien M Devos, Andrew N Doust. Genetic control and comparative genomic analysis of flowering time in setaria(poaceae)[J].G3,2013,3(2):283-295. doi:10.1534/g3.112.005207.
[13] 贾小平, 董普辉, 张红晓, 孔祥生. 不同谷子品种(系)生长发育特性及抗倒性分析[J]. 河南农业科学, 2015, 44(8):27-31.doi:10.15933/j.cnki.1004-3268.2015.08.006. Jia X P, Dong P H, Zhang H X, Kong X S. Analysis of growth and development characteristics and lodging resistance of different foxtail millet cultivars(strains)[J]. Journal of Henan Agricultural Sciences, 2015,44(8):27-31.doi:10.15933/j.cnki.1004-3268.2015.08.006.
[14] 贾小平, 袁玺垒, 陆平, 范丙友, 黄华, 戴凌峰. 中国71个谷子种质资源的灰色关联度分析及综合评价[J]. 种子, 2017, 36(9):1001-4705. doi:10.16590/j.cnki.1001-4705.2017.09.063. Jia X P, Yuan X L, Lu P, Fan B Y, Huang H, Dai L F. Grey incidence analysis and comprehensive evaluation of 71 millet germplasm resources in China[J]. Seed, 2017,36(9):1001-4705. doi:10.16590/j.cnki.1001-4705.2017.09.063.
[15] 贾小平. 谷子SSR标记的开发及遗传图谱的构建[D]. 北京:中国农业大学, 2008. Jia X P. Development and genetic mapping of SSR markers in foxtail millet(Setaria italica)[D].Beijing:China Agricultural University, 2008.
[16] Evanno G, Regnaut S, Goudet J.Detecting the number of clusters of individuals using the software STRUCTURE:a simulation study[J].Molecular Ecology,2005,14(8):2611-2620. doi:10.1111/j.1365-294X.2005.02553.x.
[17] Doust A N, Mauro-Herrera M, Hodgeand J G, Jessica S.The C₄ model grass Setaria is a short day plant with secondary long day genetic regulation[J].Frontiers in Plant Science,2017(8):1-10.doi:10.3389/fpls.
[18] Jia G, Huang X, Zhi H, Zhao Y, Zhao Q, Li W, Chai Y, Yang L, Liu K, Lu H, Zhu C, Lu Y, Zhou C, Fan D, Weng Q, Guo Y, Huang T, Zhang L, Lu T, Feng Q, Hao H, Liu H, Lu P, Zhang N, Li Y, Guo E, Wang S, Wang S, Liu J, Zhang W, Chen G, Zhang B, Li W, Wang Y, Li H, Zhao B, Li J, Diao X, Han B.A haplotype map of genomic variations and genome-wide association studies of agronomic traits in foxtail millet (Setaria italica)[J].Nature Genetics,2013,45(8):957-961.doi:10.1038/ng.2673.
[19] Gupta S, Kumari K, Muthamilarasan M, Parida S K, Prasad M.Population structure and association mapping of yield contributing agronomic traits in foxtail millet[J].Plant Cell Reports,2014,33(6):881-893. doi:10.1007/s00299-014-1564-0.
[20] Pajoro A, Severing E, Angenent G C, Immink R.Histone H3 lysine 36 methylation affects temperature-induced alternative splicing and flowering in plants[J].Genome Biology,2017,18(102):1-12. doi:10.1186/s13059-017-1235-x.
[21] Choi S C, Lee S, Kim S R,Lee Y S,Liu C Y,Cao X F,An G H.Trithorax group protein Oryza sativa Trithorax1 controls flowering time in rice via interaction with Early heading date 3[J]. Plant Physiol, 2014, 164:1326-1337. doi:10.1104/pp.113.228049.
[22] 段桂芳. 拟南芥F-box家族基因FOA2 的功能初步研究[D]. 长沙:湖南大学, 2014. Duan G F. Preliminary study on the function of F-box family gene FOA2 in Arabidopsis thaliana[D].Changsha:Hunan University, 2014.

Please choose a citation manager

Content to export