QTL Analysis of Cold Tolerance at Germination and Seedling Stages of Rice

doi:10.7668/hbnxb.201751291

Abstract

Abstract: The aim of the study was to locate the QTL of rice cold tolerance at germination and seedling stages, to identify new locus of cold tolerance gene, and to enrich the molecular genetic basis of rice cold tolerance. 144 F₈ stable recombinant inbred lines (RILs) derived from the cross between indica restorer line Luhui 99 (R99) and super japonica cultivar Shennong 265(SN265) were used to identify the QTLs of cold tolerance at germination and seedling stages. The germination rate of rice seeds and the withered degree of leaves at seedling stage were used as the standards of cold tolerance, QTLs were mapped by QTL IciMapping v3.0 software. Two QTLs controlling cold tolerance at germination stage and one QTL controlling cold tolerance at seedling stage were detected, which were located on chromosomes 3, 5 and 9, respectively, named qLTG-3, qLTG-5 and qSCT-9. The LOD values of the three QTLs were 3.60, 2.73, and 2.52, and their additive effects were 0.09,-0.10, and-0.09, respectively, which accounted for 11.02%, 14.07%, and 12.18% of phenotypic variation, respectively. Among them, qLTG-5 was located between R5M13-RS8 markers with a genetic distance of 8.0 cM on chromosomes 5, and no cold tolerance QTL was reported in this interval, so it might be a new QTL for cold tolerance at germination stage in rice. The results laid the foundation for fine mapping and cloning of related cold tolerance genes.

Key words: Rice, Hybridization between indica and japonica, Germination stage, Seedling stage, Cold tolerance, Quantitative trait loci

CLC Number:

S511.03

WANG Qi, FAN Shuxiu, GUO Jianghua, CHEN Zhaohe, LIANG Yinpei, LIU Zhenyu, YIN Yechao, WANG Jiayu. QTL Analysis of Cold Tolerance at Germination and Seedling Stages of Rice[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(1): 83-88. doi: 10.7668/hbnxb.201751291.

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