Cloning and Expression Analysis of Phytochrome Interacting Factor TaPIF4 in Wheat

doi:10.7668/hbnxb.201750739

Abstract

Abstract: In order to study the structure, characteristics and function of phytochrome interacting factor TaPIF4 gene in wheat, the TaPIF4 gene was cloned from wheat and analyzed by bioinformatics methods. We analyzed the rhythmic expression of this gene within 48 hours and its expression pattern under the stress conditions of low temperature, drought, high salt and abscisic acid (ABA) and speculated the possible biological processes by Real time fluorescence quantitative PCR (qRT-PCR). The results showed that the open reading frame (ORF) of TaPIF4 gene was 1 053 bp, and 350 amino acids were encoded. The protein encoded by this gene contained a bHLH conserved domain at the C-terminus. The bHLH domain of TaPIF4 protein was highly conserved and TaPIF4 protein was closest to the PIF4-like protein of Aegilops tauschii in evolution. The expression of TaPIF4 gene had circadian rhythm, which was characterized by low expression under light conditions and high expression under dark conditions. The expression of TaPIF4 gene was obviously inhibited by exogenous ABA. Under drought conditions, the expression of TaPIF4 increased in early stage and declined in later stage. At low temperature, the expression pattern of TaPIF4 was opposite to that of drought conditions. Under the salt stress, there was no apparent regular pattern in the expression of TaPIF4. It is speculated that TaPIF4 may be involved in plant ABA, drought and low temperature stress signal pathway.

Key words: Wheat, Phytochrome interaction factor, Photoperiod, Circadian rhythm, Stress

CLC Number:

S512.03
Q78

LI Lu, WANG Xiang, WANG Zhuangyuan, CAO Yun, HU Ge, WANG Liuyi, YIN Jun. Cloning and Expression Analysis of Phytochrome Interacting Factor TaPIF4 in Wheat[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(1): 74-82. doi: 10.7668/hbnxb.201750739.

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