Transcriptome Analysis of Protein Synthesis Related Genes in Soybean

doi:10.7668/hbnxb.201751206

Abstract

Abstract: By using transcriptome sequencing technology, the screening of protein synthesis related genes will lay a foundation for further exploring the mechanism of protein synthesis. Ji HJ117 (Protein content of 52.99%) and its backcross parent Jidou 12 (Protein content of 46.48%) with similar genetic backgrounds but different in protein content were used as research materials.Through RNA-sequencing of soybean seeds from Ji HJ117 and Jidou 12 to gain insights into the genes potentially related to protein synthesis. A total of 336 differentially expressed genes (DGEs) were screened from Ji HJ117 and Jidou 12, of which 195 were up-regulated and 141 down-regulated in Ji HJ117. Through the Gene Ontology analysis, DGEs annotated in molecular function types were mainly related to catalysis and ligation. Significant enrichment analysis of KEGG revealed that the DGEs were mainly enriched in protein processing in endoplasmic reticulum. A total of 34 DGEs were screened in this pathway, of which 33 genes were highly expressed in Ji HJ117. Nine candidate genes were selected from this pathway and their expression levels were detected by Real-time fluorescence quantitative PCR. The expression trend was basically consistent with that of RNA-Seq, confirming the reliability of RNA-Seq datas. RNA-Seq analysis obtained basic information about protein synthesis related genes in soybean. The pathway of protein processing in endoplasmic reticulum might be an important pathway that contribute to the difference of protein content in Ji HJ117 and Jidou 12. The accomplishment of these results constitute the first step toward understanding the regulation mechanisms of seed protein synthesis and provide valuable resource for further research.

Key words: Soybean, Protein content, Transcriptome sequencing, Differentially expressed genes, Fluorescent quantitative PCR

CLC Number:

S565.03
Q78

GUO Jingwen, SHI Xiaolei, LIU Qian, ZHAO Qingsong, DI Rui, LIU Bingqiang, YAN Long, WANG Fengmin, ZHANG Mengchen, ZHAO Baohua, YANG Chunyan. Transcriptome Analysis of Protein Synthesis Related Genes in Soybean[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(1): 61-73. doi: 10.7668/hbnxb.201751206.

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