Abstract: In order to clarify the functional mechanism of rice annexin OsAnn8 under drought and low temperature stress, the transcription level of OsAnn8 in rice leaves was analysed by fluorescence quantitative PCR technology. The results showed that the expression of OsAnn8 gene presented a high-low-high trend before and after drought stress, but a low-high-low-low changes before and after cold stress. Subsequently, the knocking out of OsAnn8 was also performed using the CRISPR/Cas9 gene editing techniques, and the CRISPR/Cas9 gene editing plant expression vector was transformed into the transgenic receptor variety TP309 by the agrobacterium-mediated method. A total of 32 transgenic positive plants were obtained by selection for hygromycin resistance. The analysis of sequencing map in target locus showed that overlapping peaks existed in two individual plants. Furthermore, T vector clones were sequenced separately, indicating that the monoallelic mutation happened in the two individual plants. The results indicated that monoallelic mutants had been obtained at OsAnn8 gene locus successfully. The creation of different types of mutants at OsAnn8 gene locus of rice laid the material base for gene function research under abiotic stress.

Key words: Rice, Annexin, OsAnn8, Fluorescence quantitative PCR, CRISPR/Cas9

CLC Number: 

• S511.03