Acta Agriculturae Boreali-Sinica ›› 2023, Vol. 38 ›› Issue (S1): 1-7. doi: 10.7668/hbnxb.20194099

Special Issue: Rice Biotechnology

• Crop Genetics & Breeding·Germplasm Resources·Biotechnology • Previous Articles     Next Articles

Grain Quality Characters and Metabolome Analysis of DEP1 Mutant in Rice

ZHAO Mingzhu1,2, WANG Lili1, MA Zuobin1, TANG Zhiqiang1, ZHENG Wenjing1   

  1. 1 Liaoning Rice Research Institute,Shenyang 110161,China
    2 Crop Research Institute, Liaoning Academy of Agricultural Sciences,Shenyang 110161,China
  • Received:2023-04-07 Published:2023-12-28

Abstract:

In order to determine the effect of allele variation in DEP1 locus on quality traits and metabolic profile of grains in rice,the high quality japonica cultivar Daohuaxiang and its erect panicle type mutant dep1-1 were used.The grain quality traits and differential metabolites between wild-type and mutant dep1-1 were measured.The results showed that the mutant dep1-1 had a single base A inserted into the fifth exon of the DEP1 gene,leading to premature termination of translation and the deletion of the cysteine-rich region in G-protein gamma subunit.Compared with wild-type cultivar,the grain length,appearance,viscosity,balance,taste value,peak value,recovery value and peak time of mutant dep1-1 were significantly decreased,while amylose content, acidity value and hardness were significantly increased.Compared with wild-type cultivar,37 metabolites were significantly up-regulated in dep1-1,while 45 were significantly down-regulated.KEGG analysis showed that these differential metabolites were mainly enriched in galactose metabolism,fatty acid biosynthesis,starch sucrose metabolism,linoleic acid metabolism,ABC transporters,and phenylpropanoid biosynthesis pathways.Compared with wild-type cultivar,the contents of sucrose,D-raffinose,raffinose,D-(+)-maltose D-(+),betaine and indole of grains significantly increased in dep1-1,while those of stearic acid,palmitic acid,L-leucine and indole-3-acetic acid significantly decreased.These differential metabolites preliminarily explained the physiological and biochemical mechanism of grain quality changes in dep1-1 mutant.

Key words: Rice, Erect panicle type, DEP1, Metabolome, Grain quality

Cite this article

ZHAO Mingzhu, WANG Lili, MA Zuobin, TANG Zhiqiang, ZHENG Wenjing. Grain Quality Characters and Metabolome Analysis of DEP1 Mutant in Rice[J]. Acta Agriculturae Boreali-Sinica, 2023, 38(S1): 1-7. doi: 10.7668/hbnxb.20194099.

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