Screening of Culture Conditions for the Identification of Nodulation Ability of Soybean-rhizobium Symbiosis

doi:10.7668/hbnxb.20194991

Abstract

Abstract:

In order to investigate the impact of different environments on the symbiotic nodulation ability of soybean-rhizobia and to screen suitable culture conditions for symbiotic nodulation,this study established 10 treatments based on 4 environmental factors(culture temperatures:22,26,30 ℃;culture substrates:vermiculite,vermiculiteand nutrient soil;inoculation periods:0 day post-inoculation,inoculation at 10 days;final bacterial concentration:OD₆₀₀=0.5,OD₆₀₀=0.9,OD₆₀₀=1.3),resulting in 36 different culture conditions.Five nodulation-related phenotypes of soybean-rhizobia were measured under these conditions,including the number of nodules per plant,nodule dry weight per plant,nodule size,SPAD of leaf growth at 10 and 24 days post-inoculation(DPI).Principal component analysis,fuzzy mathematics membership function method,and cluster analysis were employed to comprehensively evaluate and classify the symbiotic nodulation ability of soybean-rhizobia under different conditions.A comprehensive evaluation system for the nodulation ability of soybean-rhizobia was established,and the optimal culture conditions were selected based on the comprehensive evaluation index D value.The results showed that culture temperature was the primary environmental factor influencing the symbiotic nodulation of soybean-rhizobia.The maximum values of the number of nodules per plant,nodule dry weight per plant,nodule size,SPAD of leaf growth at 10 and 24 days post-inocubion corresponded to culture temperatures of 30,26,26,30 and 26 ℃,respectively,under,different culture conditions.Under the culture conditions set,the optimal culture temperature for the symbiotic nodulation of soybean-rhizobia was 26 ℃.Multivariate linear regression analysis and grey relational analysis based on the comprehensive evaluation index D value indicated that nodule dry weight and nodule size had a strong correlation with the symbiotic nodulation ability of soybean-rhizobia,serving as important reference indicators for nodulation ability identification.The results of this study established a comprehensive evaluation system for the symbiotic nodulation ability of soybean-rhizobia,providing a theoretical basis for selecting phenotypes and culture conditions for nodulation ability identification.

Key words: Soybean, Rhizobia, Symbiotic culture, Nodulation ability, Comprehensive evaluation

ZHAO Yuan, LIU Zhi, YANG Qing, WANG Yu, WANG Yanli, YAN Long, ZHANG Kai, SHI Xiaolei, LIU Xiaoyan. Screening of Culture Conditions for the Identification of Nodulation Ability of Soybean-rhizobium Symbiosis[J]. Acta Agriculturae Boreali-Sinica, 2024, 39(S1): 125-134. doi: 10.7668/hbnxb.20194991.

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References 27

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性状 Trait	平均值±标准差 Mean±s	最大值 Max	最小值 Min	变异系数/% CV
单株根瘤数Nodule number	18.27±12.19	57.75	4.92	66.72
单株根瘤干质量/g Nodule dry weight	0.02±0.01	0.03	7.50E-04	48.31
根瘤大小/g Nodule size	1.10E-03±7.50E-04	3.33E-03	1.47E-04	68.18
10 d叶绿素相对含量10 d SPAD	36.69±11.36	53.13	14.03	30.96
24 d叶绿素相对含量24 d SPAD	25.82±4.87	33.35	16.41	18.87

性状 Trait	平均值±标准差 Mean±s	最大值 Max	最小值 Min	变异系数/% CV
单株根瘤数Nodule number	18.27±12.19	57.75	4.92	66.72
单株根瘤干质量/g Nodule dry weight	0.02±0.01	0.03	7.50E-04	48.31
根瘤大小/g Nodule size	1.10E-03±7.50E-04	3.33E-03	1.47E-04	68.18
10 d叶绿素相对含量10 d SPAD	36.69±11.36	53.13	14.03	30.96
24 d叶绿素相对含量24 d SPAD	25.82±4.87	33.35	16.41	18.87

主成分Principal component	主成分1 PC1	主成分2 PC2
特征值Eigenvalues	2.26	1.97
贡献率Contribution	45.26	39.32
累计贡献率Cumulative contribution rate	45.26	84.58
单株根瘤数Nodule number per plant	0.74	-0.56
单株根瘤干质量Nodule dry weight per plant	0.84	0.47
根瘤大小Nodule size	0.26	0.93
10 d叶绿素相对含量10 d SPAD	0.87	0.12
24 d叶绿素相对含量24 d SPAD	-0.43	0.75

主成分Principal component	主成分1 PC1	主成分2 PC2
特征值Eigenvalues	2.26	1.97
贡献率Contribution	45.26	39.32
累计贡献率Cumulative contribution rate	45.26	84.58
单株根瘤数Nodule number per plant	0.74	-0.56
单株根瘤干质量Nodule dry weight per plant	0.84	0.47
根瘤大小Nodule size	0.26	0.93
10 d叶绿素相对含量10 d SPAD	0.87	0.12
24 d叶绿素相对含量24 d SPAD	-0.43	0.75

处理条件 Processing condition	综合指标(C) Comprehensive character indicators		隶属函数(μ) Membership function		综合得分(D) Composite scores	排名 Order
处理条件 Processing condition	X1	X2	μ1	μ2	综合得分(D) Composite scores	排名 Order
I₁₃	1.07	2.29	0.69	1.00	0.84	1
I₂₁	0.82	2.26	0.63	0.99	0.80	2
I₁₇	0.48	1.71	0.54	0.85	0.69	3
I₁₅	0.41	1.67	0.53	0.84	0.67	4
I₂₃	-0.18	1.55	0.38	0.81	0.58	5
I₃₄	2.26	-1.53	1.00	0.01	0.54	6
I₂₇	0.35	0.47	0.51	0.53	0.52	7
I₂₉	0.29	0.52	0.50	0.54	0.52	8
I₃₂	2.13	-1.58	0.97	0.00	0.52	9
I₁₆	-0.17	0.81	0.38	0.62	0.49	10
I₁₄	-0.26	0.86	0.35	0.63	0.48	11
I₃₆	1.54	-1.24	0.82	0.09	0.48	12
I₃₃	1.01	-0.67	0.68	0.23	0.47	13
I₁₉	-0.43	0.95	0.31	0.65	0.47	14
I₂₈	0.71	-0.37	0.60	0.31	0.47	15
I₂₅	0.40	-0.02	0.52	0.40	0.47	16
I₂₂	0.09	0.24	0.45	0.47	0.46	17
I₂₆	0.58	-0.34	0.57	0.32	0.45	18
I₂₄	-0.21	0.52	0.37	0.54	0.45	19
I₃₁	1.03	-1.02	0.69	0.14	0.43	20
I₁₈	-0.42	0.64	0.31	0.57	0.43	21
I₂₀	-0.09	0.12	0.40	0.44	0.42	22
I₂	0.07	-0.12	0.44	0.38	0.41	23
I₃₀	0.77	-0.93	0.62	0.17	0.41	24
I₃₅	0.38	-0.70	0.52	0.23	0.38	25
I₈	0.01	-0.41	0.43	0.30	0.37	26
I₄	-1.06	-0.52	0.15	0.27	0.21	27
I₇	-1.25	-0.41	0.10	0.30	0.19	28
I₁	-1.19	-0.49	0.12	0.28	0.19	29
I₁₁	-1.23	-0.49	0.11	0.28	0.19	30
I₉	-1.31	-0.51	0.09	0.28	0.18	31
I₆	-1.06	-0.79	0.15	0.20	0.17	32
I₃	-1.29	-0.54	0.09	0.27	0.17	33
I₅	-1.34	-0.53	0.08	0.27	0.17	34
I₁₀	-1.23	-0.66	0.11	0.24	0.17	35
I₁₂	-1.65	-0.74	0.00	0.21	0.10	36

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