Genome-Wide Association Study of Organic Acids in Vegetable Soybean

doi:10.7668/hbnxb.20194140

Abstract

Abstract:

In order to study the organic acid synthesis mechanism of vegetable soybean and provide a theoretical basis for the quality improvement of vegetable soybean,a natural population containing 264 accessions of vegetable soybean was used as experimental materials.The contents of tartaric acid,malic acid and citric acid in this population were determined by high performance liquid chromatography(HPLC)in 2020 and 2021,respectively,and genome-wide association study was conducted based on the genotype data of this population,SNP loci and candidate genes significantly associated with organic acid content were identified.The results showed that the average content of tartaric acid in 2020 and 2021 populations were 4.13,4.16 mg/g,respectively;the average content of malic acid was 7.26,8.99 mg/g,respectively;the average content of citric acid was 7.12,10.88 mg/g,respectively.Correlation analysis showed that citric acid content of 264 materials showed a significant positive correlation between 2020 and 2021.Significant positive correlation coefficients were obtained for the pairs of malic/citric acids,tartaric/malic acids,and tartaric/citric acids were 0.790^*,0.695^*,0.739^*,respectively.The results showed that there were significant differences among different varieties in the test population,with rich genetic diversity.Six specific germplasm resources with high organic acid contents were selected to provide excellent materials for the improvement and breeding of organic acid varieties of vegetable soybean.Genome-wide association study based on the mixed linear model the number of SNP loci significantly correlated with the contents of tartaric acid,malic acid and citric acid were 54,189,43,respectively,and based on the gene functional annotation information,3 and 5 candidate genes were identified which were significantly correlated with the content of tartaric acid and malic acid,respectively.

Key words: Vegetable soybean, SNP, Genome-wide association study, Organic acid, HPLC

LIU Hui, XU Wenjing, YANG Shuo, ZHANG Wei, ZHANG Hongmei, LIU Xiaoqing, ZHU Yuelin, CHEN Huatao. Genome-Wide Association Study of Organic Acids in Vegetable Soybean[J]. Acta Agriculturae Boreali-Sinica, 2023, 38(4): 74-82. doi: 10.7668/hbnxb.20194140.

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References 20

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时间/min Time	甲醇/% MeOH	0.1%磷酸水溶液/% 0.1% PBS
0	2.5	97.5
10	2.5	97.5
12	100.0	0.0
17	100.0	0.0
20	2.5	97.5
25	2.5	97.5

时间/min Time	甲醇/% MeOH	0.1%磷酸水溶液/% 0.1% PBS
0	2.5	97.5
10	2.5	97.5
12	100.0	0.0
17	100.0	0.0
20	2.5	97.5
25	2.5	97.5

有机酸 Organic acid	回归方程 Linear regression equation	相关系数(R²) Coefficient of determination	定量限/(μg/mL) Quantification limit
酒石酸 Tartaric acid	y=1 453.8x-6 643.1	0.998 2	2.5
苹果酸 Malic acid	y=715.7x-4 082.6	0.997 9	3.0
柠檬酸 Citric acid	y=1 005.8x-846.9	0.999 1	10.0

有机酸 Organic acid	回归方程 Linear regression equation	相关系数(R²) Coefficient of determination	定量限/(μg/mL) Quantification limit
酒石酸 Tartaric acid	y=1 453.8x-6 643.1	0.998 2	2.5
苹果酸 Malic acid	y=715.7x-4 082.6	0.997 9	3.0
柠檬酸 Citric acid	y=1 005.8x-846.9	0.999 1	10.0

性状 Trait	环境 Environment	最小值/ (mg/g) Min	最大值/ (mg/g) Max	均值/ (mg/g) Mean	中位数 Median	极差 Mean	标准差 s	变异系数/% CV
酒石酸	2020	1.66	9.19	4.13	3.88	7.53	1.29	31.25
Tartaric acid	2021	0.48	13.52	4.16	4.03	13.04	2.17	52.07
苹果酸	2020	1.47	36.46	7.26	6.26	34.98	4.89	67.29
Malic acid	2021	1.89	25.19	8.99	8.75	23.30	4.23	47.07
柠檬酸	2020	2.56	12.05	7.12	7.09	9.49	1.37	19.22
Citric acid	2021	1.54	18.31	10.88	11.09	16.77	2.87	26.35

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