Acta Agriculturae Boreali-Sinica ›› 2023, Vol. 38 ›› Issue (S1): 18-25. doi: 10.7668/hbnxb.20192604

Special Issue: Multigrain crops Biotechnology

• Crop Genetics & Breeding·Germplasm Resources·Biotechnology • Previous Articles     Next Articles

Establishment of Molecular Identity in 94 Foxtail Millet Varieties

LI Haiquan, JIANG Yanmiao, LIU Yanan, GENG Lingling, HAN Xiaojie, LIU Guoqing, DONG Li   

  1. Institute of Millet Crops,Hebei Academy of Agriculture and Forestry Sciences,Shijiazhuang 050035,China
  • Received:2021-08-13 Published:2023-12-28

Abstract:

To scientifically and accurately identify foxtail millet germplasm resources,strengthen the management of millet germplasm resources and protect new varieties.33 pairs of SSR markers were selected to establish molecular identity cards for 94 foxtail millet germplasm resources.A total of 500 SSR markers covering 9 linkage groups of foxtail millet chromosomes were screened,of which 33 markers showed polymorphic bands and stable amplification,these markers were used to establish molecular ID of 94 foxtail millet varieties.A total of 203 polymorphic bands were obtained.The number of alleles revealed by each marker was 3—10 with an average of 6.2.The polymorphism information content(PIC)values for the SSR loci ranged from 0.429 to 0.864 with an average of 0.740.Ten markers,including b185,b260,b224,b103,b225,CAAS1044,b186,b253,b105 and CAAS3008,were identified to be efficient in the variety ID construction.The IDs of 94 foxtail millet varieties were established by the 10 pairs of markers,which could clear distinguish all the varieties tested.

Key words: Foxtail millet, Molecular identity, Germplasm resources, SSR markers

Cite this article

LI Haiquan, JIANG Yanmiao, LIU Yanan, GENG Lingling, HAN Xiaojie, LIU Guoqing, DONG Li. Establishment of Molecular Identity in 94 Foxtail Millet Varieties[J]. Acta Agriculturae Boreali-Sinica, 2023, 38(S1): 18-25. doi: 10.7668/hbnxb.20192604.

share this article