Analysis of Endophytic Bacteria Diversity in Resistant (Susceptible) Foxtail Millet Varieties to Fusarium Head Blight

doi:10.7668/hbnxb.20194347

Abstract

Abstract:

To reveal the diversity characteristics of endophytic bacteria in different cultivars and organs,and clarify the correlation between endophytic bacteria community structure and host varieties,organ types and disease resistance and susceptible characteristics.Six different varieties of foxtail millet,Jigu 22,Honggu,Longgu No.11 and Xiaoqinggu,Shiliuzi and Nenxuan 16,which were resistant (susceptible) to Fusarium head blight,were selected as materials.The leaves,roots,stems,leaf sheaths and mature spikes of foxtail millet,were taken respectively.DNA was extracted for PCR amplification of 16S rDNA V3—V4 region,and Miseq library was constructed for high-throughput sequencing.Microbial diversity was analyzed by Major biological cloud platform.There were certain differences in endophyte species composition between susceptible and resistant varieties,the dominant populations of endophytes were Proteobacteria and Actinobacteriota,followed by Bacteroidota and Firmicutes,Myxococota and Chloroflexi were lower relative abundance followed by Gemmatimondota and Fusobacteriota.Alpha diversity analysis showed that the resistant cultivars had higher panicle community richness at leaf and maturity spikes and higher panicle diversity at leaf,root and maturity spikes.The susceptible varieties had higher richness of root and stem community and higher diversity of stem and leaf sheath;PCoA analysis showed that organ type had more effect on endophytic community structure than variety.Species composition analysis showed that the diversity of endophytic flora in foxtail millet was affected by different varieties and different parts,and the species of endophytic flora in different parts were quite different.The diversity of endophytic flora between susceptible cultivars Xiaoqinggu,Shiliuzi and Nenxuan 16 and resistant cultivars Jigu 22,Honggu and Longgu No.11 was quite different.The resistant cultivars had NB1-j in leaf sheath stage,and the ears had Acidobacteriota in mature stage.The study showed that the diversity and community structure of endophytic bacteria were affected by different organs and varieties of the foxtail millet,and the organ types had more influence on the community structure of endophytic bacteria than varieties.

Key words: Foxtail millet, Fusarium head blight, Endogenic bacteria, Diversity

MAO Yanan, TIAN Feifei, LIU Tingting, HAN Xinpeng, HAO Zhimin, LI Zhiyong. Analysis of Endophytic Bacteria Diversity in Resistant (Susceptible) Foxtail Millet Varieties to Fusarium Head Blight[J]. Acta Agriculturae Boreali-Sinica, 2024, 39(1): 175-183. doi: 10.7668/hbnxb.20194347.

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Figures/Tables 8

Tab.1 Sampling information of different sample

品种 Variety	抗病性 Disease resistance	器官 Organ
冀谷22 Jigu 22	抗赤霉病(R)	叶片、根、茎、叶鞘、成熟谷穗
红谷 Honggu	抗赤霉病(R)	叶片、根、茎、叶鞘、成熟谷穗
陇谷11号 Longgu No.11	抗赤霉病(R)	叶片、根、茎、叶鞘、成熟谷穗
小青谷 Xiaoqinggu	感赤霉病(S)	叶片、根、茎、叶鞘、成熟谷穗
石榴子 Shiliuzi	感赤霉病(S)	叶片、根、茎、叶鞘、成熟谷穗
嫩选16 Nenxuan 16	感赤霉病(S)	叶片、根、茎、叶鞘、成熟谷穗

Fig.1 Community composition of endophytic bacteria in different samples of millet G1-2.Leaf of Jigu 22;G2-2.Root of Jigu 22;G3-2.Stem of Jigu 22;G4-2.Leaf sheath of Jigu 22;G6-2.Spike of mature millet of Jigu 22;G1-4.Leaf of Xiaoqinggu;G2-4.Root of Xiaoqinggu;G3-4.Stem of Xiaoqinggu;G4-4.Leaf sheath of Xiaoqinggu;G6-4.Spike of mature millet of Xiaoqinggu;G1-5.Leaf of Shiliuzi;G2-5.Root of Shiliuzi;G3-5.Stem of Shiliuzi;G4-5.Leaf sheath of Shiliuzi;G6-5.Spike of mature millet of Shiliuzi;G1-6.Leaf of Honggu;G2-6.Root of Honggu;G3-6.Stem of Honggu;G4-6.Leaf sheath of Honggu;G6-6.Spike of mature millet of Honggu;G1-7.Leaf of Longgu No.11;G2-7.Root of Longgu No.11;G3-7.Stem of Longgu No.11;G4-7.Leaf sheath of Longgu No.11;G6-7.Spike of mature millet of Longgu No.11;G1-8.Leaf of Nenxuan 16;G2-8.Root of Nenxuan 16;G3-8.Stem of Nenxuan 16;G4-8.Leaf sheath of Nenxuan 16;G6-8.Spike of mature millet of Nenxuan 16.The same as Fig.3—4.

Fig.2 Dilution curve of millet endophytes in different samples A.Jigu 22;B.Xiaoqinggu;C.Shiliuzi;D.Honggu; E.Longgu No.11;F.Nenxuan 16.The same as Fig.4—5.

Fig.3 Analysis of Alpha diversity index of millet endophytes of different sample A—E.Leaf,root,stem,leaf sheath,spike of mature millet.The same as Fig.6.

Fig.4 PCoA analysis of endophytes of millet in different sample

Fig.5 Veen analysis of the OTU in different millet varieties

Tab.2 Different endophytes in various parts of different millet varieties

项目 Item	叶片 Leaf	根 Root	茎 Stem	叶鞘 Leaf sheath	成熟谷穗 Spike of mature millet
冀谷22(R)Jigu 22	—	—	脱硫菌门	—	—
红谷(R) Honggu	梭杆菌门	拉氏杆菌门	—	—	拉氏杆菌门
陇谷11号(R)Longgu No.11	拉氏杆菌门	—	—	—	疣微菌门
小青谷(S)Xiaoqinggu	—	—	髌骨细菌门
	—	—	甲基肌酐门
			NB1-j
石榴子(S)Shiliuzi	—	—	—	Dependentiae	—
嫩选16(S)Nenxuan 16	—	—	—	—	—

Fig.6 Veen analysis of OTU in different organs of mille

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