Analysis of Differentially Expressed Protein of M239 in Response to Verticillium Wilt Pathogen Infection

doi:10.7668/hbnxb.20194359

Abstract

Abstract:

In order to explore the mechanism of plant in response to Verticillium wilt pathogen infection,Dali wild eggplant (M239) was took as the material.By the artificial inoculation of Verticillium dahliae,four key physiological indicators of M239 root were determined at 0,6,12,24,and 48 h after inoculation with V.dahliae, which namely the peroxidase (POD) activity,catalase (CAT) activity,phenylalanine ammonilyase (PAL) activity and the soluble protein (SP) content.The result showed that 12 and 24 h after inoculation with V.dahliae were determined as the proper sampling time points for proteome analysis.On this basis,iTRAQ technique was used to analyze the protein changes of M239 root after inoculation with V.dahliae.Compared with CK,463 differentially expressed proteins (DEPs) were found in M239 root at 12 h after inoculation with V.dahliae,among which 305 DEPs were down-regulated and 158 were up-regulated.At 24 h after inoculation with V.dahliae in M239,456 DEPs were induced to express by pathogens,including 296 down-regulated and 160 up-regulated proteins.These proteins were mainly enriched in carbon metabolism,glyoxylate and dicarboxylic metabolism,biosynthesis of secondary metabolites,pyruvate metabolism,and metabolic pathways.Compared with the reported Verticillium wilt resistant materials,it was found that the number of proteins and metabolic pathways involved in the defense response of M239 in the process of responding to the infection of V.dahliae was less.Therefore,it failed to form an effective defense network,and finally manifested as susceptible to the disease.

Key words: Wild eggplant, Verticillium wilt, Verticillium dahliae, Isobaric tag for relative and absolute quantitation (iTRAQ), Differentially expressed proteins

YU Yanan, LI Zhibin, GONG Yaju, BAO Rui, GUI Min, DU Guanghui, LIU Jiaxun, WU Liyan. Analysis of Differentially Expressed Protein of M239 in Response to Verticillium Wilt Pathogen Infection[J]. Acta Agriculturae Boreali-Sinica, 2024, 39(1): 165-174. doi: 10.7668/hbnxb.20194359.

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References 42

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编号 No.	KEGG富集通路(ID) Enriched KEGG pathways	差异表达蛋白DEPs		P值 P value
编号 No.	KEGG富集通路(ID) Enriched KEGG pathways	上调Up	下调Down	P值 P value
1	碳代谢(sly01200)	14	13	3.09×10^-7
2	乙醛酸盐和二羧酸代谢(sly00630)	8	4	5.16×10^-6
3	次生代谢物的生物合成(sly01110)	28	35	1.86×10^-6
4	丙酮酸代谢(sly00620)	6	6	7.71×10^-5
5	新陈代谢(sly01100)	41	47	2.41×10^-3
6	氨酰生物合成(sly00970)	4	5	2.42×10^-3
7	柠檬酸循环(sly00020)	4	3	2.78×10^-3
8	氨基酸的生物合成(sly01230)	5	11	5.56×10^-3
9	蛋白酶体(sly03050)	2	4	6.21×10^-3
10	糖解与糖代谢合成(sly00010)	4	6	1.18×10^-3
11	缬氨酸、亮氨酸和异亮氨酸的生物合成(sly00290)	0	4	1.19×10^-2
12	光合生物中的碳固定(sly00710)	2	5	1.64×10^-2
13	有机含硒化合物代谢(sly00450)	0	3	1.80×10^-2
14	α亚麻酸代谢(sly00592)	4	1	2.36×10^-2
15	脂肪酸代谢(sly01212)	5	2	2.80×10^-2
16	脂肪酸降解(sly00071)	5	0	2.96×10^-2
17	c5支链二元酸代谢(sly00660)	0	2	4.25×10^-2
18	单菌霉素生物合成(sly00261)	0	2	4.25×10^-2

编号 No.	KEGG富集通路(ID) Enriched KEGG pathways	差异表达蛋白DEPs		P值 P value
编号 No.	KEGG富集通路(ID) Enriched KEGG pathways	上调Up	下调Down	P值 P value
1	碳代谢(sly01200)	14	13	3.09×10^-7
2	乙醛酸盐和二羧酸代谢(sly00630)	8	4	5.16×10^-6
3	次生代谢物的生物合成(sly01110)	28	35	1.86×10^-6
4	丙酮酸代谢(sly00620)	6	6	7.71×10^-5
5	新陈代谢(sly01100)	41	47	2.41×10^-3
6	氨酰生物合成(sly00970)	4	5	2.42×10^-3
7	柠檬酸循环(sly00020)	4	3	2.78×10^-3
8	氨基酸的生物合成(sly01230)	5	11	5.56×10^-3
9	蛋白酶体(sly03050)	2	4	6.21×10^-3
10	糖解与糖代谢合成(sly00010)	4	6	1.18×10^-3
11	缬氨酸、亮氨酸和异亮氨酸的生物合成(sly00290)	0	4	1.19×10^-2
12	光合生物中的碳固定(sly00710)	2	5	1.64×10^-2
13	有机含硒化合物代谢(sly00450)	0	3	1.80×10^-2
14	α亚麻酸代谢(sly00592)	4	1	2.36×10^-2
15	脂肪酸代谢(sly01212)	5	2	2.80×10^-2
16	脂肪酸降解(sly00071)	5	0	2.96×10^-2
17	c5支链二元酸代谢(sly00660)	0	2	4.25×10^-2
18	单菌霉素生物合成(sly00261)	0	2	4.25×10^-2

编号 No.	KEGG富集通路(ID) Enriched KEGG pathways	差异表达蛋白DEPs		P值 P value
编号 No.	KEGG富集通路(ID) Enriched KEGG pathways	上调Up	下调Down	P值 P value
1	糖酵解/糖异生(sly00010)	8	7	8.65×10^-5
2	新陈代谢(sly01100)	34	68	1.49×10^-4
3	次生代谢物的生物合成(sly01110)	23	41	3.33×10^-4
4	氨基酸的生物合成(sly01230)	8	11	1.18×10^-3
5	碳代谢(sly01200)	7	13	2.87×10^-3
6	果糖和甘露糖代谢(sly00051)	2	6	4.66×10^-3
7	柠檬酸循环(sly00020)	2	5	4.97×10^-3
8	蛋白酶体(sly03050)	4	2	1.02×10^-2
9	苯丙类生物合成(sly00940)	4	10	1.69×10^-2
10	嘌呤代谢(sly00230)	6	5	2.41×10^-2
11	丙酮酸代谢(sly00620)	4	4	2.64×10^-2
12	氰胺酸代谢(sly00460)	2	3	2.75×10^-2
13	非同源末端连接(sly03450)	0	2	3.36×10^-2
14	丙氨酸、天冬氨酸和谷氨酸代谢(sly00250)	1	4	4.36×10^-2

编号 No.	KEGG富集通路(ID) Enriched KEGG pathways	差异表达蛋白DEPs		P值 P value
编号 No.	KEGG富集通路(ID) Enriched KEGG pathways	上调Up	下调Down	P值 P value
1	糖酵解/糖异生(sly00010)	8	7	8.65×10^-5
2	新陈代谢(sly01100)	34	68	1.49×10^-4
3	次生代谢物的生物合成(sly01110)	23	41	3.33×10^-4
4	氨基酸的生物合成(sly01230)	8	11	1.18×10^-3
5	碳代谢(sly01200)	7	13	2.87×10^-3
6	果糖和甘露糖代谢(sly00051)	2	6	4.66×10^-3
7	柠檬酸循环(sly00020)	2	5	4.97×10^-3
8	蛋白酶体(sly03050)	4	2	1.02×10^-2
9	苯丙类生物合成(sly00940)	4	10	1.69×10^-2
10	嘌呤代谢(sly00230)	6	5	2.41×10^-2
11	丙酮酸代谢(sly00620)	4	4	2.64×10^-2
12	氰胺酸代谢(sly00460)	2	3	2.75×10^-2
13	非同源末端连接(sly03450)	0	2	3.36×10^-2
14	丙氨酸、天冬氨酸和谷氨酸代谢(sly00250)	1	4	4.36×10^-2

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