Resistance of GhWRKY48 Negatively Regulated Cotton Against Verticillium dahlia

doi:10.7668/hbnxb.201751632

Abstract

Abstract: Since Verticillium wilt causes great loss in cotton production every year, it is an economical and effective method to identify resistance genes for breeding new varieties to control Verticillium wilt. A WRKY transcription factor gene was cloned from upland cotton, named GhWRKY48. The full-length of GhWRKY48 cDNA was 882 bp,encoding 293 amino acid residues with a predicted molecular mass of 32.68 ku and an isoelectric point (pI) of 6.10. qPCR analyses showed that GhWRKY48 gene constitutively expressed in root, stem and leaf, and preferentially done in stem. In addition, GhWRKY48 expression could be induced by V. dahliae infection, jasminic acid and salicylic acid. To dissect the defense function, GhWRKY48 -silenced plants were developed by virus-induced gene silencing techniques, which were then inoculated with V. dahliae. The results showed that the resistance of these silenced plants significantly increased compared to the control. The rates of diseased plants and disease index in GhWRKY48 -silenced plants were lower than in the control. All together, GhWRKY48 was a negative transcription factor participating in plant resistance to V. dahliae. Therefore, the GhWRKY48 gene could be used as an ideal candidate gene for cotton defense breeding.

Key words: Gossypium hirsutum L., GhWRKY48, Gene silencing, Verticillium dahliae, Disease-resistance

CLC Number:

S562.03

LIU Jianfen, LEI Yu, ZHANG Zhennan, HU Guang, TANG Ye, ZHANG Ning, SI Huaijun, WU Jiahe. Resistance of GhWRKY48 Negatively Regulated Cotton Against Verticillium dahlia[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(5): 99-105. doi: 10.7668/hbnxb.201751632.

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http://www.hbnxb.net/EN/Y2019/V34/I5/99

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