Abstract: In order to narrow population range to be screened, reduce the workload and improve the selection efficiency of sterile and maintainer lines in the three-line cross breeding of pepper, the sequence polymorphism sites of cytoplasmic fertility marker SCAR130 was used to design KASP primer to transform SCAR130 into KASP130 molecular marker. The three lines material (male sterile line, maintainer line, restorer line) and F₁ hybrids of pepper CMS were selected as the experimental subjects, and the KASP130 molecular marker were applied into detection of pepper cytoplasm type and its stability and reliability were tested through two detection platforms of real time PCR machine LC480 and the SNPline of LGC Company, respectively. The results showed that KASP130 marker, same as SCAR130, could accurately divide the tested pepper materials into fertile cytoplasm(N) and sterile cytoplasm(S). Furthermore, in the marker-assisted selection breeding, KASP130 was also successfully applied to the early identification of cytoplasmic fertility of pepper as well as the backcross breeding of pepper maintainer lines and male sterile lines. In conclusion, cytoplasmic fertility marker SCAR130 has been successfully transformed into KASP130 molecular marker. It can identify the cytoplasm type of pepper, which lays a foundation for application of KASP130 in three-line cross breeding programe of peppers.

Key words: Pepper, Cytoplasmic male sterily(CMS), Three-line seed production system, KASP

CLC Number: 

• S641.03