Bioinformation Analysis and Expression Analysis of NHX Genes Family in Pepper

doi:10.7668/hbnxb.20191999

Abstract

Abstract: To understand the bioinformatics function of the NHX gene family in pepper, explore its gene expression characteristics under abiotic stress, and provide a basis for the function development of pepper CaNHX gene and the pepper salt and drought resistance breeding genes. The pepper NHX gene family was identified by bioinformatics methods, and the gene structure, domains, phylogenetic relationships, gene expression patterns and abiotic stress expression were analyzed. The results showed that there were 7 GME family members in pepper, which could be divided into 3 subgroups, and all contained Na⁺/H⁺ exchange conserved domains;they were mainly distributed on 6 chromosomes, and their gene sizes had obvious differences. The number was mainly maintained between 198-952, and the exon content was between 7-21. All proteins except CaNHX5 had no signal peptides and were hydrophobic proteins, which were most likely to be located in the vacuole. They were all transmembrane proteins;Motif analysis found that the N-terminal contained the zinc finger structure of CXC24XC, and the C-terminal contained Trp(W)-24 and TrKA-N, the highly conserved secondary structure in the sequence was mainly irregular coils and α-helices. Multi-sequence evolutionary tree construction analyses indicated that aminoacid sequence encoded by this gene was closely related to tomato. The results of qRT-PCR analysis showed that under osmotic and salt stress, the gene expression of CaNHX6 increased significantly, which was 13.5 and 13.6 times that at 0 h, respectively;after treatment with methyl jasmonate and gibberellin.The expression of CaNHX5 and CaNHX6 in pepper leaves showed a significant up-regulation trend, while the others were on the contrary. The different expressions indicated that they played a role in abiotic stress.

Key words: Pepper, NHX gene family, Expression analysis, Stress

CLC Number:

Q78
S641.03

LUO Jian, XU Chunmiao, ZHANG Guobin, YU Jihua. Bioinformation Analysis and Expression Analysis of NHX Genes Family in Pepper[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(3): 15-24. doi: 10.7668/hbnxb.20191999.

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