Cloning and Expression Analysis of HbDHAR2 Gene from Hevea brasiliensis

doi:10.7668/hbnxb.20191875

Abstract

Abstract: Dehydroascorbate reductase (DHAR) plays an important role in plant growth, development, and abiotic stress tolerance by regulating ascorbic acid level. To reveal the sequence characteristic, expression pattern and possible biological function of DHAR gene in Hevea brasiliensis, HbDHAR2 was cloned from Hevea brasiliensis by RT-PCR. HbDHAR2 contained an open reading frame of 639 bp encoding a protein of 212 amino acid residues with an isoelectric point of 5.69 and a calculated molecular weight of 23.82 ku. HbDHAR2 was a hydrophilic protein. It contained a GST N-terminal domain and a GST C-terminal DHAR domain and belonged to the DHAR subclass of GST super family. Sequence alignment analysis indicated that HbDHAR2 showed high sequence identity with cassava MeDHAR2 and castor bean RcDHAR2. Tissue expression profiling revealed that HbDHAR2 was expressed in root, bark, latex, mature leaf, senescent leaf, new shoot, female flower and male flower of Hevea brasiliensis, with the highest expression in new shoot and the lowest expression in root. HbDHAR2 expression was induced by ethephon (ETH), methyl jasmonate (MeJA), salicylic acid (SA), abscisic acid (ABA), hydrogen peroxide (H₂O₂), methyl viologen (MV), cold, drought and salt treatments, indicating that HbDHAR2 might function in rubber tree tolerance to abiotic stress. These results lay a foundation for further studying the function of HbDHAR2 in Hevea brasiliensis.

Key words: Hevea brasiliensis, Dehydroascorbate reductase, Cloning, Expression analysis, Abiotic stress

CLC Number:

S794.1
Q78

LI Shuangjiang, FENG Chengtian, HU Yiyu, YUAN Kun, LIU Jinping, WANG Zhenhui, LIU Hui. Cloning and Expression Analysis of HbDHAR2 Gene from Hevea brasiliensis[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(3): 25-32. doi: 10.7668/hbnxb.20191875.

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